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In situ gel for injection containing polymyocytes and preparation method thereof

A technology of in situ gel and polyinocytes, which is applied in the direction of medical preparations containing active ingredients, medical preparations with inactive ingredients, pharmaceutical formulas, etc., can solve the limitations of the development and Application, easy precipitation, solubility drop, etc., to achieve the effect of improving animal compliance, simple preparation process, and long-lasting effect

Active Publication Date: 2018-06-22
NANJING DEPOT VETERINARY PHARMA R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the preparation process, it was found that the solubility of polyinogenic cells in the temperature-sensitive in situ gel matrix decreased, and it was easy to precipitate and poor stability, which limited the development and application of in situ gels for injection of polyinogenic cells.

Method used

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  • In situ gel for injection containing polymyocytes and preparation method thereof
  • In situ gel for injection containing polymyocytes and preparation method thereof
  • In situ gel for injection containing polymyocytes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Polymyocyte Microcapsules

[0029] prescription:

[0030]

[0031] Preparation method: (a), take the prescribed amount of gelatin and gum arabic and dissolve them in 800ml of water for injection respectively to obtain gelatin aqueous solution and gum arabic aqueous solution, mix the two solutions at a volume ratio of 1:1, keep the temperature at 55°C, Continue to stir; (b), dissolving the polymyocytes of the prescription amount in the gelatin-gum arabic mixed solution under stirring, continue to stir for 30 minutes; (c), add 10% glacial acetic acid solution of the prescription amount, adjust the solution The pH of the solution is between 3-4, take a sample to observe the formation of capsules, add 4400ml of water for injection after formation of capsules, stir evenly, place in an ice bath and quickly cool to below 10°C, add 30mL of formaldehyde solution, and stir for 30 minutes; (d ), adding 10% sodium hydroxide solution to adjust the pH of the solution at 8-9, soli...

Embodiment 2

[0037] In situ gel for polysarcoma injection (each 1mL contains 5 mg of polysarcoma)

[0038] The prescription is as follows:

[0039]

[0040] Preparation method: (a), take prescription amount of disodium hydrogen phosphate, sodium dihydrogen phosphate, dissolve in 700mL water for injection to prepare a buffer solution with pH6.8, dissolve the prescription amount of potassium sorbate in the buffer solution, and then add Stir the prescribed amount of polyinosin powder evenly to obtain solution A; (b), sprinkle the prescribed amount of gel matrix P407, P188 and hydroxypropyl methylcellulose on solution A, and refrigerate at about 4°C until obtained Clarify, no agglomerate, uniformly dispersed solution, filter to obtain solution B; (c), add the polyinocytic microcapsules of the prescribed amount into solution B, stir until the dispersion is uniform, add the remaining amount of water for injection, mix Homogenize and sterilize to obtain 1000mL in situ gel for injection contai...

Embodiment 3

[0042] In situ gel for polysarcoma injection (each 1mL contains 6 mg of polysarcoma)

[0043] The prescription is as follows:

[0044]

[0045]

[0046] Preparation method: (a) Dissolve disodium hydrogen phosphate and sodium dihydrogen phosphate in the prescribed amount, dissolve them in 700mL water for injection to prepare a pH6.9 buffer, dissolve the prescribed amount of potassium sorbate in the buffer, and add the prescribed amount Amount of polymyocyte powder, stirred evenly, to obtain solution A; (b) Sprinkle prescription amount of gel matrix P407, P188 and povidone on solution A, and refrigerate at about 4°C until a clear, lump-free, The uniformly dispersed solution is filtered to obtain solution B; (c) add the prescribed amount of polysarcoma microcapsules to solution B, stir until uniformly dispersed, add the remaining amount of water for injection, mix well, and sterilize to obtain 1000mL in situ gel for injection containing polymyocytes; finally dispensed acco...

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Abstract

The invention discloses a polyinosinic acid-polycytidylic acid (poly I:C)-containing in-situ gel for injection; the poly I:C-containing in-situ gel for injection includes the following components: 0.05-0.8 part by weight of a poly I:C powder, 0.1-5.0 parts by weight of poly I:C microcapsules, 12.0-27.0 parts by weight of P407, 0.4-15.0 parts by weight of P188, 0.01-10.0 parts by weight of a polymer retardant, 0.01-2.0 parts by weight of a preservative, and 50.0-90.0 parts by weight of water for injection, wherein an acid-base buffer agent adjusts the pH of the in-situ gel for injection to 5.5-7.5. The invention also discloses a preparation method of the poly I:C-containing in-situ gel for injection. The in-situ gel for injection solves the problem of poorer compatibility of poly I:C in a gel matrix, and has convenient drug administration; when drug administration is performed through intramuscular injection or subcutaneous injection, the in-situ gel can be quickly transformed into a gel within 15 s in an injected part, the lasting time is up to 144 h, and an effective blood concentration with relatively stable state can be maintained for a long time after one-time drug administration.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations for animals, and relates to an in situ gel for injection containing polymyocytes and a preparation method thereof, in particular to an in situ gel for injection containing polymyocytes with a suitable phase transition temperature and its preparation method. Background technique [0002] Under the conditions of intensive and large-scale breeding, high-density breeding environment is poor, infectious diseases occur frequently, antibiotics and other drugs are abused, and nutrition is difficult to balance, and animal stress is frequent, which makes the basic immunity of livestock and poultry low, which increases the probability of disease and aggravates the disease. illness. Viral diseases are especially difficult to prevent and control due to the lack of vaccines and the high price of biological products. Therefore, it is imminent to develop high-efficiency immune-enhancing drugs, especial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K31/713A61K47/42A61K47/38A61K47/32C08J9/28C08G12/06A61P31/14A61P31/16A61P31/22A61P31/20A61P37/04A61P35/00
Inventor 余祖功郭凡溪
Owner NANJING DEPOT VETERINARY PHARMA R&D CO LTD
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