Skin patch for repairing skin burn and preparing method of skin patch

A technology for repairing skin and skin, which is applied in the fields of medical formula, medical science, prosthesis, etc., can solve the problems of immune rejection response efficacy and efficiency, and achieve the effects of not easy to accumulate pigment to form scars, quickly repair skin, and not easy to immune rejection

Inactive Publication Date: 2016-05-25
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is, aiming at defects such as the use of allogeneic skin matrix to repair skin burn wounds in the prior art, which is easy to cause immune r

Method used

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  • Skin patch for repairing skin burn and preparing method of skin patch

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preparation example Construction

[0023] Specifically, the preparation method of the skin patch for repairing skin burns provided by the present invention comprises the following steps:

[0024] S1. Obtaining fat stem cells and allogeneic skin matrix respectively;

[0025] Among them, the fat stem cells are derived from the autologous body of patients with skin burns to reduce immune rejection; the allogeneic skin matrix is ​​the skin matrix after decellularization, which can be allogeneic skin matrix (that is, human skin matrix other than skin burn patients), It can also be a heterogeneous skin matrix (such as pig, cow and other animal skin matrix);

[0026] S2. The adipose stem cells are mixed with the hydrogel and inoculated on the allogeneic skin matrix;

[0027] The allogeneic skin matrix is ​​not only used as a patch for repairing burnt skin, but also retains the insoluble components in the original tissue structure, mainly including collagen, elastin, aminodextran and structural proteins, etc. Restora...

Embodiment 1

[0057] A kind of skin patch for repairing skin burn provided by the present invention, its preparation method comprises the following steps:

[0058] S1a. Patient selection;

[0059] Inclusion criteria for burn patients: patients with second to third degree burn wounds, and the following patients were excluded:

[0060] Pregnant and lactating women; those who are allergic to biological agents used in treatment; severe inhalation injury and other important organ injuries, such as: liver and kidney function injury, craniocerebral injury, etc.; serious basic diseases such as: diabetes, hypertension, tumor or Chronic organ damage, etc.; those who hinder the signing and understanding of informed consent.

[0061] S2a, before treatment, check and evaluate the patient;

[0062] Evaluation criteria: blood routine test (WBC>3.0×10*9 / L, LC>15%; Hb>80g / L; PLT>60×10*9 / L), liver and kidney function, hepatitis B three lines (surface antigen negative ), hepatitis C antibody negative, AIDS...

Embodiment 2

[0088] The difference from Embodiment 1 is that in this embodiment,

[0089] S3b, the acquisition of fat stem cells;

[0090] S31b, collecting adipose tissue;

[0091] Select patients who meet the evaluation criteria in step S2a, use a liposuction machine to extract adipose tissue from the patient's abdomen, buttocks, thighs and other fat-rich parts under aseptic conditions, remove the blood vessels and fibers that are visible to the naked eye in the fat, and wash the fat with PBS The tissue was put into the buffer solution containing the double antibody (penicillin / streptomycin, each with 1% content) for 3 times, and stored under the condition of 4° C. in a sterile environment for future use.

[0092] S32b, obtaining primary adipose stem cells;

[0093] Take the adipose tissue obtained in step S31b, shred it under sterile conditions, add 0.075% collagenase type Ⅰ, digest at 37°C for 20 minutes on a shaking shaker with a vibration speed of 50 rpm, and then add an equal volum...

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Abstract

The invention relates to a preparing method of a skin patch for repairing a skin burn. The preparing method comprises the following steps that adipose-derived stem cells and an allogenic skin matrix are obtained; the adipose-derived stem cells and hydrogel are mixed and inoculated to the allogenic skin matrix, wherein the adipose-derived stem cells are from the body of a patient suffering from the skin burn, and the hydrogel is prepared by mixing an alginate solution and a gelatin solution. The adipose-derived stem cells are carried in the hydrogel prepared from alginate and gelatin so that bacteria can be blocked, a humid environment is maintained for the adipose-derived stem cells and the skin wound, a good repair environment is provided, and immunological rejection is relieved; in addition, adhesion of the patch and the wound can be prevented when the patch is replaced, and thus the new skin tissue can be protected; the purpose of quickly repairing the skin can be achieved, the pain of the patient can also be relieved, and the phenomena of a scar caused by pigment accumulation at the wound part and the like are not likely to occur.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a skin patch for repairing skin burns and a preparation method thereof. Background technique [0002] The skin is the barrier between the human body and the external environment. It not only plays the roles of protection, secretion, metabolism and sensation, but also participates in the immune response and maintains the stability of the internal environment of the body. As the most exposed skin of the human body, the incidence of injury and large-scale burn accidents is relatively high. Early burn wound coverage can inhibit the growth of bacteria, prevent infection, and prevent the loss of water and electrolytes; autologous skin transplantation is the first choice for the treatment of burns, but for large area burns, autologous skin cannot meet the needs, and the treatment cycle is long. , the skin wound is prone to infection; while the allogeneic skin matrix will cause obvious...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/60A61L27/20A61L27/22
CPCA61L27/362A61L27/20A61L27/222A61L27/3691A61L27/3834A61L27/60A61L2400/18
Inventor 曾宪卓张哲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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