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High-temperature-resistant and strong-emulsification microorganism strain and application of microorganism strain in paraffin removing and preventing

A technology for removing wax and bacterial strains, which is applied in the field of petroleum microorganisms and can solve problems such as poor high temperature resistance

Active Publication Date: 2016-05-25
唐山新新石油科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been widely used in countries with advanced technology in the world, and the research and application of microbial oil recovery technology are also being carried out in China, but most of the strains are poor in high temperature resistance. For example, the Chinese invention patent CN201210137276.5 discloses a strain resistant to high temperature paraffin degradation Bacteria and its application in wax removal and anti-wax, the wax removal rate of Microbacterium NS02 in this invention is 59%, and it can grow under the condition of 30~70℃

Method used

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  • High-temperature-resistant and strong-emulsification microorganism strain and application of microorganism strain in paraffin removing and preventing
  • High-temperature-resistant and strong-emulsification microorganism strain and application of microorganism strain in paraffin removing and preventing
  • High-temperature-resistant and strong-emulsification microorganism strain and application of microorganism strain in paraffin removing and preventing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Screening and Identification of Strains with Preservation Number CCTCCNo: M2015695

[0092] 210 oil and water samples were collected in the oil field, and 18 strains of Qingfangwax were obtained through enrichment culture, and then through directional domestication and compatibility optimization of crude oil, eight strains were finally selected, namely 98-32, 98-11, XG- 2. NG-23, SG-3, G104-5-1, G104-5-2 and G104-5-3.

[0093] Streak the selected strains on the nutrient agar plate, culture at 37°C for 48 hours, place at room temperature for 24 hours to observe and describe the characteristics of the colony and take pictures, select a typical single colony to make hanging drops, and observe its motility; at the same time, use Gram staining, Observe the shape of the cells and measure their size. Electron microscope observation thalline cell flagella situation and taking pictures, the results are shown in Table 1, wherein the photos of the bacterial classification of the ...

Embodiment 2

[0099] The production method of the anti-wax bacteria agent with the preservation number CCTCCNo: M2015695

[0100] A production method for preparing a wax-clearing and anti-wax liquid microbial agent with a preservation number of CCTCCNo: M2015695, the steps are as follows:

[0101] 1) Slant culture: inoculate the strain with deposit number CCTCCNo: M2015695 in the slant medium, and culture at 25-28°C for 3 days.

[0102] The slant medium formula is: 0.5% beef extract, 1.0% peptone, 0.5% NaCl, 1.5%-1.8% agar powder, the balance is water, pH7.2-7.4.

[0103] 2) Shake flask culture: inoculate the cultured strains on the inclined plane into the liquid medium, the inoculum amount is 5-10%, the rotation speed is 140r / min, cultivate at 37°C for 24 hours, pH6.5-7.5, and the bacterial concentration is 2-7× 10 8 pieces / ml.

[0104] The formula of the liquid medium is: liquid paraffin 2.0%, NH 4 Cl0.4%, Na 2 HPO 4 0.4%, KH 2 PO 4 0.4%, MgSO 40.1%, yeast powder 0.001%, the bala...

Embodiment 3

[0113] Performance evaluation of the strain agent with deposit number CCTCCNo: M2015695

[0114] 1. High temperature resistance

[0115] Inject the bacterial solution into the test tube aseptically, seal it, and treat it at 90°C for 10 days.

[0116] Calculate the number of viable bacteria before and after treatment to obtain the survival rate (%). In the absence of fresh nutrient supply, more than 70% still survive after static treatment at 90°C for 10 days. During the screening process, strains whose survival rate was lower than 70% were eliminated.

[0117] 2. Emulsifying effect on crude oil

[0118] Add 100ml of formation water, a small amount of inorganic salt and 5g of crude oil to a 250ml Erlenmeyer flask, inoculate 10ml of bacterial solution, seal it with a leather stopper, place it in a constant temperature water bath at 65°C for 7 days, and compare it with the blank control to observe the emulsification and dispersion of microorganisms after they act on crude oil ...

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Abstract

The invention relates to the technical field of petroleum microorganisms, and particularly discloses a high-temperature-resistant and strong-emulsification microorganism strain and application of the microorganism strain in paraffin removing and preventing. A paraffin removing and preventing strain is preserved in the China Center for Type Culture Collection and has the preservation number of CCTCC NO:M2015695. The microorganism strain solves the problems that an existing microorganism strain is poor in high temperature resistance, not good in raw petroleum emulsification effect, generally low in paraffin removing rate and high in petroleum production cost. The paraffin removing rate of the paraffin removing and preventing strain reaches up to 71.4-80.6%, and the paraffin removing and preventing strain can grow at the temperature of 90 DEG C, has high emulsification capacity, can effectively reduce raw petroleum viscosity and surface tension and prevent paraffin crystals from being accumulated on a well wall, has effects on preventing and removing paraffin, improves petroleum well yield, and has wide application value in actual production of a petroleum field.

Description

technical field [0001] The invention relates to the technical field of petroleum microbes, and specifically discloses a high-temperature-resistant strong emulsifying microbial strain and its application in removing wax. Background technique [0002] During the crude oil extraction process, the wax in the crude oil is precipitated due to the reduction of pressure and temperature, forming crystals, which adhere to the near wellbore, wellbore and pump valves, etc., affecting the normal operation of the extraction and liquid extraction system. Periodic wax removal operations are required to remove wax crystals attached to the wellbore extraction system. [0003] Microbial oil recovery technology refers to various technologies that use microorganisms and their metabolites to increase crude oil production and recovery. Its application scope involves underground oil displacement, formation plugging removal and viscosity reduction, wellbore wax removal and wax prevention, crude oil...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K8/524C12R1/01
CPCC09K8/524C12N1/20C12N1/205C12R2001/01
Inventor 余守德刘如林杜建龙郭彦生郭守泉柴丽敏叶盛军刘伟王芳兰汤蒙云恩龙李超峰杨辉梅万会高新成李旭詹伟
Owner 唐山新新石油科技开发有限公司
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