A kind of extraction method of microalgae oil
An extraction method and a technology for microalgae oil, which are applied in the directions of oil/fat production, fat production, etc., can solve problems such as limited effect of alcohol dissolution, and achieve the effects of lightening load, high yield and simple operation process
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Embodiment 1
[0026] The microalgae is chlorella vulgaris, which was cultured in a 10L photobioreactor for 10 days. At the end of the culture, the concentration of algae cells was analyzed to be 1.82g / L (dry weight basis). Take 5L of the microalgae liquid and carry out centrifugation, control the centrifugal rotation speed to 4000 rpm, and centrifuge for 3 minutes to obtain the microalgae mud. Add 0.03mol / L Na to the microalgae mud 2 CO 3 solution 45ml and 10% sodium hydroxide solution 45ml. The above system was heated to 60 ºC while stirring, and kept for 100 min to break the cells and saponify the oil. Then use a membrane with a pore size of 10 -1 μm microfiltration membrane to remove algae cells and debris. Continue to add 1mol / L Na to the liquid system 2 CO 3 Solution 90ml, make fatty acid salt precipitate, filter and collect solid; Dissolve fatty acid salt solid in a small amount of water, add hydrochloric acid solution and acidify to pH 2.0, obtain free fatty acid; Centrifuge th...
Embodiment 2
[0028] The microalgae is botrytis, which was cultured in a 10L photobioreactor for 10 days. At the end of the culture, the concentration of algae cells was analyzed to be 1.78g / L (dry weight basis). Take 5L of microalgae liquid for centrifugation, control the centrifugation speed to 4000rpm, and centrifuge for 5 minutes to obtain microalgae mud, dry and grind the microalgae mud to obtain 8.9g of microalgae powder. Add 0.02mol / L NaNO to the microalgae powder 3 Solution 58ml and 30% sodium hydroxide solution 29ml. The above system was heated to 70 ºC while stirring, and kept for 120 min to carry out cell disruption and oil saponification. Then use a membrane with a pore size of 10 -1 μm microfiltration membrane to remove algae cells and debris. Continue to add 1.5 mol / L NaNO to the liquid system 3 Solution 45ml, make fatty acid salt precipitate, filter and collect solid; Dissolve fatty acid salt solid in a small amount of water, add hydrochloric acid solution and acidify to ...
Embodiment 3
[0030] The microalgae is botrytis, which was cultured in a 10L photobioreactor for 10 days. At the end of the culture, the concentration of algae cells was analyzed to be 1.92g / L (dry weight basis). Take 5L of microalgae liquid for centrifugation, control the centrifugation speed to 4000rpm, and centrifuge for 5 minutes to obtain microalgae mud, dry and grind the microalgae mud to obtain 9.6g of microalgae powder. Add 0.03mol / L of KH to the microalgae powder 2 PO 4 solution 40ml and 10% potassium hydroxide solution 40ml. The above system was heated to 50 ºC while stirring, and kept for 120 min to carry out cell disruption and oil saponification. Then use a membrane with a pore size of 10 -1 μm microfiltration membrane to remove algae cells and debris. Continue to add 1.5mol / L of KH to the liquid system 2 PO 4 Solution 48ml, make fatty acid salt precipitate, filter and collect solid; Dissolve fatty acid salt solid in a small amount of water, add hydrochloric acid solution...
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