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A magnetic covalent organic framework nanocomposite material and its preparation method and application

A technology of covalent organic skeleton and nanocomposite materials, applied in the fields of peptide preparation, organic chemistry, chemical instruments and methods, etc., can solve the problems of unsatisfactory effect and unstable structure in the enrichment of hydrophobic peptides, and achieve High yield, rapid enrichment and separation, easy separation effect

Active Publication Date: 2018-05-04
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, MOFs materials also have structural instability and other problems, and the enrichment of hydrophobic peptides is not ideal. Therefore, it is necessary to develop a material with a more stable structure to enrich hydrophobic peptides.

Method used

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  • A magnetic covalent organic framework nanocomposite material and its preparation method and application
  • A magnetic covalent organic framework nanocomposite material and its preparation method and application
  • A magnetic covalent organic framework nanocomposite material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The synthesis of magnetic covalent organic framework nanocomposite, it comprises the steps:

[0035] (1) 8.1g FeCl 3 ·6H 2 O and 12.0g dispersant sodium acetate are added to 200mL ethylene glycol solution and mixed;

[0036] (2) Add 1.5g trisodium citrate dihydrate as electrostatic stabilizer;

[0037] (3) Then move the solution into an airtight heating container, and carry out a solvothermal reaction at 200°C for 8 hours to obtain ferric oxide nanoparticles;

[0038] (4) Disperse 50 mg of ferric oxide nanoparticles synthesized in 15 mL of isopropanol,

[0039] (5) Add 26.5 mg of 1,3,5-tris(4-aminophenyl)benzene and 15 mg of terephthalaldehyde, and disperse evenly by ultrasonication for 25 minutes at room temperature;

[0040] (6) Continue to sonicate, add 0.5mL acetic acid while sonicating, and then continue to sonicate for 20min.

[0041] figure 1 A is a transmission electron microscope image of the iron ferric oxide nanoparticles synthesized in Example 1. It ca...

Embodiment 2

[0045] Magnetic covalent organic framework nanocomposite for the enrichment of peptides containing benzene ring structure

[0046] A series of 5 mg magnetic covalent organic framework nanocomposites synthesized in Example 1 were weighed into 5 mL centrifuge tubes, and mixed with 1 mL of different concentrations of peptide L2 (FGFG) and peptide L7 (RPPGFSPFR) respectively. The mixture was placed on a shaker at room temperature for 2 h to ensure adsorption equilibrium. After adsorption equilibrium, a magnet was used to separate the mixture of magnetic particles and peptides, and the absorbance of the supernatant after the reaction was tested by a UV-visible spectrophotometer to calculate the adsorption amount. Such as Figure 4 As shown, the saturated adsorption capacity of magnetic covalent organic framework nanocomposites to peptide L7 is greater than 7.5 mg / g, while the saturated adsorption capacity of peptide L2 is only 1.5 mg / g, indicating that magnetic covalent organic fr...

Embodiment 3

[0048] Magnetic covalent organic framework nanocomposites for separation and enrichment of peptides containing benzene ring structure and actual serum samples

[0049] Weigh a series of 5 mg magnetic covalent organic framework nanocomposites synthesized in Example 1 in a 5 mL centrifuge tube and mix with 1 mL standard solution (containing 50-fold diluted healthy human serum and 50 ppm of L7(RPPGFSPFR)) . The mixture was kept on a shaker at room temperature for 2 h to ensure adsorption equilibrium. After adsorption equilibrium, a magnet was used to separate the mixture of magnetic particles and peptides, and the supernatant was collected. The adsorbed material was eluted with 200ul eluent (50% acetonitrile + 50% water), mixed evenly, kept on a shaker for 1 hour, and the eluate was collected. Chromatographic separation of standards, supernatants and eluents was performed by high performance liquid chromatography. Figure 5 It is the chromatogram of standard solution, supernat...

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Abstract

The invention discloses a magnetic covalent organic framework nanocomposite material and a preparation method and application. The method comprises the steps that by means of a solvothermal reaction method, ferroferric oxide nanoparticles, 1,3,5-tris(4-aminophenyl)benzene and terephthalaldehyde serve as the raw materials, and the magnetic covalent organic framework nanocomposite material capable of being used for separating and enriching peptide fragments with benzene ring structures or hydrophobic peptide fragments is prepared under the catalytic action of acetic acid. The preparation process is simple and effective, reagent consumption is low, and the yield is high; the obtained magnetic covalent organic framework nanocomposite material not only has the advantages of covalent organic framework materials of being porous, stable in structure, large in specific surface area and the like, but also has the advantages of magnetic materials of being good in dispersity, easy to separate, good in magnetic inductivity and the like, and the magnetic covalent organic framework nanocomposite material has a good application prospect in the fields of proteomics, peptideomics and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic-organic composite materials and analysis, and specifically relates to a magnetic covalent organic framework nanocomposite material, a preparation method and an application thereof. Background technique [0002] The identification of low-abundance proteins or peptides is one of the difficulties in proteomics research. Currently, the enrichment and separation methods for low-abundance proteins and peptides mainly include organic solvent precipitation, ultrafiltration, solvent evaporation, electroelution enrichment, chromatography, and solid-phase microextraction. These methods have a series of problems such as complex process, cumbersome operation, easy loss of low-abundance proteins or peptides, and easy introduction of impurities. These problems will affect the enrichment of endogenous peptides, so the development of an efficient and convenient separation and enrichment method has become an urg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/22B01J20/30B01J20/28C07K1/14
CPCB01J20/06B01J20/223B01J20/28009B01J2220/46C07K1/14
Inventor 林子俺林国
Owner FUZHOU UNIV
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