Extraction process and application of grifolan

A technique of extracting polysaccharides from Grifola frondosa, applied to medical preparations containing active ingredients, plant raw materials, digestive system, etc., to achieve the effects of promoting proliferation, promoting healing and repair, and improving extraction efficiency

Inactive Publication Date: 2016-06-15
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of pharmacological studies have proved that Grifola frondosa polysaccharide (GFP) has significant anti-tumor, hypoglycemic, hypolipidemic, inhibitin

Method used

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  • Extraction process and application of grifolan
  • Extraction process and application of grifolan
  • Extraction process and application of grifolan

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1: Extraction of Grifola frondosa polysaccharide

[0032] Take the dry product of Grifola frondosa fruit body, crush it and pass a 10-mesh sieve to obtain Grifola frondosa fruit body particles, add 10 times the amount (mass to volume ratio) of water to slightly boil for 2h; filter, filter the residue with 8 times the amount of 2% NaOH The solution is leached for 2 hours; filtered, the filtrate is concentrated to Brix (soluble solids) ≥ 15%, and after centrifugation at 10000 rpm for 10 minutes, the supernatant is pumped into the ultrafiltration separation module with molecular weight cutoff of 100kDa, 30kDa, and 5kDa, that is, the molecular weight cutoff is 100kDa. The filtrate of the filtration separation module continues to be pumped into the ultrafiltration separation module with a molecular weight cut-off of 30kDa, and the filtrate of the ultrafiltration separation module with a molecular weight cut-off of 30kDa continues to be pumped into the ultrafiltration sep...

Example Embodiment

[0035] Example 2: The effect of Grifola frondosa polysaccharide on the proliferation of human gastric mucosal epithelial cells

[0036] 1. Material

[0037] Cell line: Human normal gastric mucosal epithelial cell GES-1 was purchased from Beijing Institute of Cancer Prevention and Treatment.

[0038] Medium: The basic medium for GES-1 cell culture is DMEM medium (10% FBS, 100 U penicillin, 100 U streptomycin).

[0039] Grifola frondosa polysaccharide GFP (polysaccharide of Grifolafrondosa): The polysaccharide of Grifola frondosa prepared in Example 1 is used.

[0040] 2. Method

[0041] The MTS method was used to detect the effect of GFP on the proliferation activity of GES-1. Take the logarithmic growth phase GES-1 and adjust its concentration to 2.0×10 after trypsin digestion 4 Pcs / mL, seeded in 96-well plate, 100μL per well, placed at 37℃, saturated humidity, 5% CO 2 After culturing in a sterile incubator for 24 hours, discard the old culture medium, and add 200 μL each of GFP solutio...

Example Embodiment

[0047] Example 3: The effect of Grifola frondosa polysaccharide on the healing and repair of human gastric mucosa wounds

[0048] 1. Material: Same as Example 2

[0049] 2. Method

[0050] Select GES-1 in logarithmic growth phase, with 6×10 per hole 4 Each cell was seeded in a 24-well plate with 400μL per well. Set at 37℃, 5% CO 2 After culturing in an incubator for 24 hours, after the cells have formed a tighter connection into a single layer, use a capillary with a diameter of 1.5 mm to draw a mark vertically in the center of each hole of the 24-well plate, and both wounds can be seen under the microscope. side. After scratching, aspirate the original culture medium, wash each well with 1mL PBS twice to remove non-adherent cells, refer to the results of the proliferation experiment, select the final concentration of GFP to be 2, 0.4, 0.08 mg / mL, 400 μL of culture medium, and add only to the control group DMEM basal medium 400μL. Each concentration group has 4 parallel multiple ...

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Abstract

The invention discloses an extraction process and application of grifolan. The grifolan is obtained by adopting a method with the steps of water extraction, alkaline extraction, ultrafiltration and alcohol precipitation; it is discovered that the grifolan can promote gastric mucosa epithelial cells to proliferate and migrate to a gastric mucosa wound area by up-regulating mRNA and protein expression level of TFF2 and protein expression level of EGF and down-regulating mRNA and protein expression level of TGF-beta1; thus the aim of promoting gastric mucosa healing and plerosis is achieved, and a basis is provided for developing and using grifola frondosa and overcoming gastric mucosa wounds and chronic gastritis.

Description

technical field [0001] The present invention relates to the extraction process and application of Grifola frondosa polysaccharide. Background technique [0002] The gastric mucosa has a tissue structure that prevents gastric juice from digesting itself and the stimulation of various damage factors, and protects the physiological functions of gastric mucosal cells. The integrity of this barrier is an important basis for the protection of gastric mucosa. Gastric mucosa is often caused by chemical factors (drinking, strong tea, coffee, smoking and irritating drugs such as aspirin, indomethacin, etc.), physical factors (too cold, too hot, too rough food or overeating), bacteria or their toxins. The cause of the irritation caused the injury. The clinical manifestations are a series of symptoms such as pain and discomfort in the epigastric cavity, fullness, belching, acid reflux, stomach burning, nausea, and vomiting. Gastric mucosal changes of different degrees often appear in ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/07A61P1/04
CPCC08B37/0003A61K31/715A61K36/07A61K2236/331A61K2236/39A61K2236/51A61K2236/53
Inventor 潘裕添王丽菊黄家福洪绯
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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