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Extraction process of Grifola frondosa polysaccharide and its application in preparation of medicine for repairing gastric mucosal trauma

A technique of extracting polysaccharides from Grifola frondosa, which is applied in the direction of drug combinations, medical preparations containing active ingredients, and pharmaceutical formulas, to achieve the effects of promoting proliferation, promoting healing and repair, and avoiding hydrolysis loss

Active Publication Date: 2018-11-13
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of pharmacological studies have proved that Grifola frondosa polysaccharide (GFP) has significant anti-tumor, hypoglycemic, hypolipidemic, inhibiting hepatitis B DNA replication, anti-HIV virus, anti-oxidation and improving immune system functions. Reports on its application in repairing gastric mucosal trauma

Method used

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  • Extraction process of Grifola frondosa polysaccharide and its application in preparation of medicine for repairing gastric mucosal trauma
  • Extraction process of Grifola frondosa polysaccharide and its application in preparation of medicine for repairing gastric mucosal trauma
  • Extraction process of Grifola frondosa polysaccharide and its application in preparation of medicine for repairing gastric mucosal trauma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Extraction of Grifola frondosa polysaccharide

[0032]Take the dry fruiting body of Grifola frondosa, pass through a 10-mesh sieve to obtain fruiting body particles of Grifola frondosa, add 10 times the amount (mass to volume ratio) of water and boil for 2 hours, filter, and concentrate the filtrate until Brix (soluble solids) ≥ 15 %, after centrifugation at 10000rpm for 10min, the supernatant is pumped successively into ultrafiltration separation modules with molecular weight cutoffs of 100kDa, 30kDa, and 5kDa, that is, the filtrate from the ultrafiltration separation module with a molecular weight cutoff of 100kDa continues to be pumped into an ultrafiltration separation module with a molecular weight cutoff of 30kDa , the filtrate of the ultrafiltration separation module with a molecular weight cut-off of 30kDa continues to be pumped into the ultrafiltration separation module with a molecular weight cut-off of 5kDa, and the retentate and filtrate of each...

Embodiment 2

[0035] Example 2: Effects of Grifola frondosa polysaccharides on the proliferation of human gastric mucosal epithelial cells

[0036] 1. Materials

[0037] Cell line: human normal gastric mucosal epithelial cells GES-1 were purchased from Beijing Institute of Cancer Prevention and Control.

[0038] Medium: The basal medium for GES-1 cell culture is DMEM medium (10% FBS, 100U penicillin, 100U streptomycin).

[0039] Grifola frondosa polysaccharide GFP (polysaccharide of Grifola frondosa): the Grifola frondosa polysaccharide prepared in Example 1 was used.

[0040] 2. Method

[0041] The effect of GFP on the proliferation activity of GES-1 was detected by MTS method. GES-1 in the logarithmic growth phase was digested with trypsin and its concentration was adjusted to 2.0×10 4 cells / mL, seeded in a 96-well plate, 100 μL per well, placed at 37°C, saturated humidity, 5% CO 2 After 24 hours of culture in a sterile incubator, the old culture medium was discarded. The experimenta...

Embodiment 3

[0047] Example 3: Effects of Grifola frondosa polysaccharides on human gastric mucosal wound healing and repair

[0048] 1. Material: Same as Example 2

[0049] 2. Method

[0050] Select GES-1 in the logarithmic growth phase, and use 6×10 per well 4 Cells were seeded in 24-well plates, 400 μL per well. Set at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours. After the cells form a relatively tight connection to form a single layer, use a capillary tube with a diameter of 1.5 mm to draw a vertical trace in the center of each well of the 24-well plate, and the two sides of the wound can be seen under the microscope at the same time. side. Aspirate the original culture medium after scratching, wash each well twice with 1mL PBS to remove non-adherent cells, select the final concentration of GFP as 2, 0.4, 0.08mg / mL with reference to the results of the proliferation experiment, and culture medium 400μL, the control group only Add 400 μL of DMEM basal medium. For each con...

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Abstract

The invention discloses an extraction process and application of polysaccharide of grifola frondosa to preparation of a medicament for repairing gastric mucosa damage. The polysaccharide of the grifola frondosa is obtained by a method comprising water extraction, ultra-filtration and alcohol precipitation, and is found to promote proliferation and migration of gastric epithelial cells to a damaged area of gastric mucosa by up-regulating the mRNA and protein expression level of TFF2 and the protein expression level of EGF and down-regulating the mRNA and protein expression level and the like of TGF-beta1, thereby fulfilling the aim of promoting healing and repair of the gastric mucosa and providing a basis for development and utilization of the grifola frondosa and overcoming of gastric mucosa damage and chronic gastritis.

Description

technical field [0001] The invention relates to the extraction process of the polysaccharide from Grifola frondosa and its use in the preparation of medicine for repairing gastric mucosal wounds. Background technique [0002] The gastric mucosa has a tissue structure that prevents gastric juice from digesting itself and the stimulation of various damage factors, and protects the physiological functions of gastric mucosal cells. The integrity of this barrier is an important basis for the protection of gastric mucosa. Gastric mucosa is often caused by chemical factors (drinking, strong tea, coffee, smoking and irritating drugs such as aspirin, indomethacin, etc.), physical factors (too cold, too hot, too rough food or overeating), bacteria or their toxins. The cause of the irritation caused the injury. The clinical manifestations are a series of symptoms such as pain and discomfort in the epigastric cavity, fullness, belching, acid reflux, stomach burning, nausea, and vomitin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/04
CPCA61K31/715C08B37/0003
Inventor 潘裕添王丽菊黄家福洪绯
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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