A method for extracting rapeseed oil and inactivating glucosidase from rapeseed

A technology of rapeseed oil and rapeseed, applied in the field of extracting rapeseed oil and inactivating glucosidase, which can solve the problems of high content of glucosinolate and its degradation products in cake, waste of high-quality protein resources, and low extraction rate of free oil , to achieve the effect of shortening the oil production cycle, eliminating the use of enzymes, and reducing the detoxification process

Active Publication Date: 2020-04-07
JIANGNAN UNIV
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  • Description
  • Claims
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for extracting rapeseed oil and inactivating glucosidase from rapeseed, aiming to solve the problem of high content of glucosinolate and its degradation products, low extraction rate of free oil, and residues caused by traditional techniques. Problems such as high residual oil rate in the phase, waste of high-quality protein resources, large enzyme consumption, high cost or serious environmental pollution

Method used

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  • A method for extracting rapeseed oil and inactivating glucosidase from rapeseed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The dried rapeseed is not subjected to any pretreatment, but only crushed. It is pre-crushed with a Chinese medicine pulverizer, and then pulverized with a three-roll mill to a volume average particle size of about 45 μm to obtain the raw material for oil extraction.

[0039] The oil extraction raw materials were adjusted to pH 5.0 at a ratio of material to water of 1:6 (w / v) at 50°C, and 3% pectinase, cellulase, and xylanase were added for enzymatic hydrolysis for 3 hours. After the enzymolysis, the pH was adjusted to 9.0 with 2N sodium hydroxide, the temperature was increased to 60°C, and the alkaline extraction was performed for 1.5 hours. After the alkaline extraction, 3% alkaline protease was added and the enzymatic hydrolysis was continued for 1 hour. After the reaction is finished, it is centrifuged at 6000 rpm for 10 minutes to obtain four incompatible phases-free oil, emulsion, water phase and residue phase. Free rapeseed oil is obtained and weighed.

[0040] The f...

Embodiment 2

[0042] Dry rapeseeds are directly subjected to pressure wet heat treatment. Put the soaked rapeseed 2 into an autoclave for pressure wet heat treatment. The pressure is 0.50Mpa, the temperature is 110℃, and the time is set to 20min. Collect the treated rapeseed 3.

[0043] The rapeseed after steam explosion is dried in a blast drying oven at 50°C; it is pre-pulverized with a Chinese medicine grinder, and then pulverized with a three-roll grinder to a volume average particle size of about 28 μm to obtain the oil-extracting raw material.

[0044] The oil extraction raw materials were adjusted to pH 5.0 at a ratio of material to water of 1:6 (w / v) at 50°C, and 3% pectinase, cellulase, and xylanase were added for enzymatic hydrolysis for 3 hours. After enzymolysis, the pH was adjusted to 9.5 with 2N sodium hydroxide, the temperature was increased to 60°C, and the alkaline extraction was performed for 1.5 hours. After the alkaline extraction, 3% alkaline protease was added and the enzy...

Embodiment 3

[0048] Dried rapeseed 1 was soaked in deionized water for 5 hours according to the material-to-water ratio of 1:2 (w / v), drained and discarded the filtrate, and collected the soaked rapeseed 2.

[0049] The soaked rapeseed 2 is subjected to steam explosion treatment, and its steam explosion pressure is set to 1.5MPa±0.1MPa, and the pressure holding time is 180s. After the blasting is over, open the steam blaster collection bin to collect the rapeseed 3 after steam explosion.

[0050] The rapeseed 3 after steam explosion was dried in a blast drying oven at 50°C; it was pre-pulverized with a Chinese medicine pulverizer, and then pulverized with a three-roll mill to a volume average particle size of about 21 μm to obtain the oil extraction raw material I.

[0051] The obtained raw material I was adjusted to pH 9.0 with 2N sodium hydroxide solution at 70°C according to the material-to-liquid ratio of 1:6 (w / v), and the concentration of ethanol aqueous solution was 50% (v / v). Time 3h. A...

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Abstract

The invention discloses a method for extracting rapeseed oil and passivated myrosinase from rapeseeds and belongs to the technical field of agricultural resources.The method has the advantages that raw materials are soaked by deionized water, moisture in cells is turned into steam during steam explosion or pressurization heat-moisture treatment, and cell walls are broken to allow the cell structure to be loose and promote the dissolution of grease in the cells; an ethanol solution is used as the oil extraction medium, glucosinolates and most degradation products are allowed to enter a water phase, the glucosinolate and glucosinolate degradation product contents in cake are lowered, and the detoxification procedure of rapeseed cake is reduced; enzyme use is avoided, oil production cycle is shortened, production cost is reduced, oil product quality is optimized, and the problems that the glucosinolate and glucosinolate degradation product contents of the cake obtained by a traditional rapeseed process are high, high-quality protein resources are wasted, a large amount of enzyme needs to be used, cost is high or environment pollution is severe, and the like are solved.

Description

Technical field [0001] The invention relates to a method for extracting rapeseed oil and inactivating glucosidase from rapeseed, and belongs to the technical field of agricultural resources. Background technique [0002] Rapeseed is one of the main oil crops in my country. The annual output of rapeseed in the country exceeds 14 million tons. The provinces of the Yangtze River Basin are the main producing areas of rapeseed. Rapeseed contains 40%-45% oil, 20%-25% protein and 25% carbohydrates. Rapeseed oil is a nutrient-rich oil. The content of unsaturated fatty acids is more than 80%. Among them, oleic acid is more than 40%. It is easily absorbed by the human body. It has good frying and antioxidant properties. It is long-term consumption and prevents arteriosclerosis. , Promote microvascular circulation, prevent colon cancer, reduce the content of serum cholesterol in the human body, reduce blood pressure and lose weight, especially for obesity, hypertension, heart disease, arte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/04C11B1/10C11B3/16A23D9/04
CPCA23D9/04C11B1/04C11B1/10C11B3/16
Inventor 杨瑞金任燕勤李鹏飞张益奇张文斌赵伟华霄
Owner JIANGNAN UNIV
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