A superhydrophobic micropit array chip and its preparation method and application

A micropit array and super-hydrophobic technology, applied in biochemical equipment and methods, biochemical instruments, urinary tract/kidney cells, etc., can solve the problems of low efficiency, complicated operation, time-consuming and laborious, etc., and achieve convenient cultivation and maintenance, biological Good compatibility and avoid cross-contamination effect

Active Publication Date: 2018-05-11
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, there is currently no platform that can be effectively used for high-throughput and high-resolution imaging. In the past, high-resolution imaging of multiple samples was performed by processing samples one by one, which was time-consuming, laborious, and inefficient, which largely limited high-resolution Development of Resolution Imaging
[0018] Finally, the operations of various high-throughput cell microarray devices are mostly too complicated, such as adding samples or changing liquids one by one, which also has a great impact on the application of these cell microarray devices.

Method used

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  • A superhydrophobic micropit array chip and its preparation method and application
  • A superhydrophobic micropit array chip and its preparation method and application
  • A superhydrophobic micropit array chip and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1, preparation of superhydrophobic micropit array chip by micrografting

[0083] Schematic diagram of the structure of the superhydrophobic micropit array chip. figure 1 As shown, it includes a micro-pit array layer 1 and a super-hydrophobic layer 2 bonded on the surface of the micro-pit array layer, wherein the thickness of the super-hydrophobic layer 2 is 100 microns.

[0084] like figure 1 As shown, the superhydrophobic micropit array chip is prepared by micrografting technology, and the specific steps are as follows:

[0085] (a) Uniform glue: Taking Dow Corning 3140 as an example, a very thin layer of glue can be obtained on the PMMA layer at a speed of 7000rpm for 30s.

[0086] (b) PDMS micropit chip preparation: use the processed silicon wafer SU-8 mold (customized and processed by Boao Biochip Company), wrap it in tin foil and use it as an inverted mold container for later use. Preparation of PDMS prepolymer solution, the volume ratio of monomer and c...

Embodiment 2

[0093] Example 2. Preparation of superhydrophobic micropit array chip by in situ synthesis

[0094] Schematic diagram (side view) of the structure of the superhydrophobic micropit array chip. Figure 5 As shown, it includes a base layer 1 and a superhydrophobic microporous array layer 2 attached to the surface of the substrate (the microporous array layer 2 is made of a superhydrophobic material).

[0095] like Image 6 As shown, the superhydrophobic micropit array chip was prepared by in situ synthesis technology, and the specific steps were as follows:

[0096] (a) Preparation of negative gel microcolumn array: uniformly 100 μm thick negative gel was applied on the silanized glass slide, exposed and developed to obtain a microcolumn array with a height of 100 μm, a diameter of 500 μm, and a center distance of 1000 μm.

[0097] (b) In-situ polymerization of the superhydrophobic layer: Align the glass slide with the micropit array with another silanized glass slide and clamp...

Embodiment 3

[0099] Example 3. Superhydrophobic micropit array chip for ultra-high resolution imaging

[0100] This embodiment is based on the unique requirements of super-resolution imaging, and the preparation method of the micro-pit substrate array has been adjusted accordingly. The structure diagram of the super-hydrophobic micro-pit array chip and figure 1 Same, including the micro-pit array layer 1 and the super-hydrophobic layer 2 bonded on the surface of the micro-pit array layer, only the micro-pit array layer 1 is composed of a base layer (special optical glass slide for ultra-high resolution imaging) and bonded on the substrate Composition of the microwell array layer on it.

[0101] Production process such as Figure 8 As shown, the specific steps are as follows:

[0102] (a) Uniform glue: Taking Dow Corning 3140 as an example, a very thin layer of glue can be obtained on the PMMA layer at a speed of 7000rpm for 30s.

[0103] (b) Photoresist dry film microarray preparation: ...

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Abstract

A super-hydrophobic micro-pit array chip, a preparation method therefor, and applications thereof. The micro-pit array chip comprises a micro-pit array layer, and the surface on which micro-pits are formed, of the micro-pit array layer is a super-hydrophobic surface except micro-pits or the bottoms of the micro-pits. The micro-pit array chip may be: 1) the surface except the micro-pits is the super-hydrophobic surface, and the micro-pit array chip comprises the micro-pit array layer and a super-hydrophobic layer attached to the surface of the micro-pit array layer; and 2) the surface except the bottoms of the micro-pits is a super-hydrophobic surface, the micro-pit array layer comprises a substrate layer and a micropore array layer attached to the surface of a substrate, and micropore array layer is made of a super-hydrophobic material. By means of the micro-pit array chip, complete isolation among cell microarrays can be implemented at a very low cost in the cell microarray construction process, cross contamination among cell arrays is avoided, good biocompatibility is ensured, and the micro-pit array chip has no obvious impact on normal growth of various cells and is suitable for high-throughput cell detection and analysis.

Description

technical field [0001] The invention relates to a super-hydrophobic micropit array chip, a preparation method and application thereof. Background technique [0002] Cell experiments are one of the most basic research methods in biological research. Many biological studies, such as high-throughput drug screening, combined stem cell screening, etc., involve a large number of cytological experiments, through a large number of cell processing processes that are often very similar to optimize certain conditions or results; traditional experimental operations often These cell experiments are done manually in multi-well plates, which is time-consuming and labor-intensive, consumes a lot of reagents, and research costs are high. [0003] Cell microarray is a tiny cell array formed by chemical and physical separation. Such cell microarrays have attracted more and more attention in biological research because of their high throughput, low reagent consumption, and low cost. Chemical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12N5/071C12N5/09
CPCC12M23/12C12M23/20C12N5/0686C12N5/069C12N5/0694C12M3/00
Inventor 刘鹏张鹏飞张健雄边升太程一淳
Owner TSINGHUA UNIV
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