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Screening and application of marine schizophyllum commune strain

A technology of Schizophyllum and Schizophyllin, which is applied in the directions of application, medical preparations containing active ingredients, fungi, etc., to achieve the effect of outstanding moisturizing performance and great application and promotion value

Active Publication Date: 2016-08-24
NINGBO XINUOYA MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been no reports of Schizophyllum strains growing in marine resources

Method used

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  • Screening and application of marine schizophyllum commune strain
  • Screening and application of marine schizophyllum commune strain
  • Screening and application of marine schizophyllum commune strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Screening of white ginseng strains from marine sources

[0020] 1. Medium preparation

[0021] Use natural seawater or artificially prepared seawater to prepare modified PDA medium: 200g potato, 20g glucose, 15-20g agar, 1000ml seawater or artificial seawater, natural PH. The method is to wash and peel, then weigh 200g potatoes and cut them into small pieces, add sea water to boil (boil for 20-30 minutes, the potato pieces can be punctured by a glass rod), filter with eight layers of gauze, and heat. Then add 1-20g agar according to the actual experiment needs, continue to heat and stir to mix, after the agar is dissolved, add glucose, stir evenly, after a little cooling, add sea water or artificial sea water to 1000 ml, divide into Erlenmeyer flasks, and stopper , Wrap, (121℃) sterilize for about 20 minutes, take it out and shake it, cool to about 60℃, prepare a flat plate, store it in a refrigerator at 4℃ for later use after solidification.

[0022] 2. Screening...

Embodiment 2

[0024] Example 2: Identification of White Ginseng Strains

[0025] 1. Extraction of fungal DNA genome

[0026] Take the strain culture solution obtained in Example 1, and add appropriate amount of dH to the precipitate after centrifugation 2 O is fully ground and broken, and DNA extraction is performed using TaKaRa's commercial fungal nucleic acid extraction kit.

[0027] 2. Determination of nucleic acid purity and concentration

[0028] Take a certain amount of DNA extract and dilute it by a certain multiple, and then measure the OD value at 260, 280 and 320nm respectively, and calculate the nucleic acid purity by (OD260-OD320) / (OD280-OD320). The purity range of natural nucleic acid is 116~210 , Generally 118±012 is appropriate; nucleic acid concentration (ng / μL)≈50×(OD260-OD320) / L×D (L is the optical path length cm, D is the dilution multiple), according to the results, dilute the nucleic acid concentration to a suitable The template concentration for PCR is 100~300ng / μL.

[0029] 3...

Embodiment 3

[0037] Example 3: Schizophyllin production

[0038] 1. Fermentation culture of Schizophyllum

[0039] Schizophyllum is inoculated into the fermentation medium at an inoculation ratio of 5-10%, and cultured in a shake flask at 25-27°C and 160-180rpm for 24 to 36 hours. The fermentation medium formula is: glucose 30.0g / L, yeast powder 5.0g / L, potassium dihydrogen phosphate 0.5g / L, and magnesium sulfate 0.3g / L.

[0040] 2. Schizophyllin extraction and purification

[0041] Using Schizophyllum fermentation culture as the material, the fermentation broth was collected, centrifuged at 8 000 r / min for 20 min, and the sediment was discarded. Add powdered activated carbon to the supernatant, heat and stir for 15 minutes in a water bath, and filter with suction to obtain a viscous, transparent liquid. The decolorized fermentation broth was deproteinized by the Sevag method. After repeating 5 times, 2.5 times the volume of 95% ethanol was added to the deproteinized fermentation broth, and a la...

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Abstract

The invention provides a macro fungus, namely white ginseng, derived from coastal mudflat; the macro marine fungus is identified to be schizophyllum commune which is capable of growing in a high-salinity culture medium and is capable of generating schizophyllan. The marine schizophyllum commune disclosed by the invention is screened by virtue of a natural seawater prepared restrictive medium by taking rotten woods from coastal and seaside wetlands as samples; and the schizophyllum commune is subjected to liquid fermentation, so that the marine schizophyllan is obtained. The chemical component of the schizophyllan produced by the invention is beta-1,3 / 1,6-glucan, which is derived from the marine schizophyllum commune and is applicable to the fields of food, cosmetics and medicines; and the schizophyllan is suitable for industrial production and is quite high in application and popularization values.

Description

Technical field [0001] The invention relates to the field of marine microorganisms and their natural products, in particular to the screening and cultivation of marine white ginseng fungi, the extraction and purification of schizophyllin produced, and the qualitative and quantitative analysis of schizophyllin. Background technique [0002] Marine biological resources are due to the greater pressure of the water in the deep sea, lack of oxygen or even oxygen, continuous low temperature, occasional high temperature or cold springs, and the polar ocean environment is extremely extreme, cold, high salt and strong radiation. Marine organisms that survive and multiply in such an extreme marine environment must have a life system that adapts to the extreme living environment. Therefore, extreme environmental marine organisms are not only unique in species, but also contain many potential active substances and genetic resources, which have huge prospects for scientific research and comme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/08A23K20/163A61K8/60A61K31/716C12R1/645
CPCA61K8/60A61K31/716C12P19/08C12N1/145C12R2001/645
Inventor 田健诸辉
Owner NINGBO XINUOYA MARINE BIOTECH CO LTD
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