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A dietary supplement of probiotics and plant extracts capable of alleviating lead toxicity

A dietary supplement and plant extract technology, applied to the functions of food ingredients, bacteria and lactobacilli used in food preparation, etc., can solve problems such as pain and nausea, relieve mental decline, and relieve oxidative stress damage , the effect of long shelf life

Active Publication Date: 2019-03-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dimercaptosuccinic acid (DMSA), a competitive antidote of DMSA, may cause certain side effects such as upper and middle abdominal pain and nausea after being absorbed into the human body

Method used

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  • A dietary supplement of probiotics and plant extracts capable of alleviating lead toxicity
  • A dietary supplement of probiotics and plant extracts capable of alleviating lead toxicity
  • A dietary supplement of probiotics and plant extracts capable of alleviating lead toxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: The impact of different freeze-drying protective agents on the survival rate of plantaractobacillus in the freeze-drying process

[0055] After activating Lactobacillus plantarum CGMCC 5494 in MRS liquid medium for two generations, it was inserted into 1 LMRS liquid medium according to the inoculum size of 2%, and placed at 37° C. for constant temperature expansion for 18 hours. Centrifuge at 5000 g for 15 min at 4°C, discard the supernatant to collect the cells, wash the cells twice with sterile phosphate buffered saline (PBS, 60 mmol / L, pH 6.5), and collect the cells. The above experiment was repeated 5 times, and 5 parts of bacteria were collected, and 25mL of sterile PBS solution, 150g / L sucrose solution, 100g / L trehalose solution, 50g / L sorbitol solution, and 100g / L skimmed milk powder solution were added as freeze-dried The protective agent was mixed and resuspended. Dispense 1 mL of the bacterial suspension containing the protective agent into ster...

Embodiment 2

[0058] Example 2: Effect of trehalose concentration on freeze-dried activity of Lactobacillus plantarum CGMCC 5494 cells

[0059] Protectant solutions with different concentration gradients of trehalose concentrations of 100g / L, 150g / L, 200g / L, and 250g / L were prepared, and the expanded culture, centrifugation, washing and freezing of Lactobacillus plantarum CGMCC 5494 were carried out according to the process of Example 1 Drying, counting of living bacteria to investigate the effect of the concentration of trehalose on the freeze-drying activity of CGMCC 5494 cells. The results are attached image 3 Shown:

[0060] pass image 3 It can be seen that with the increase of trehalose concentration, the protective effect is significantly improved. However, experiments have found that when the concentration of trehalose is greater than 150g / L, the bacterium after freeze-drying has a high viscosity and cannot grind the bacterial powder. Suitable.

Embodiment 3

[0061] Example 3: The effect of the concentration of skim milk trehalose on the freeze-dried activity of Lactobacillus plantarum CGMCC 5494 cells

[0062] Preparation of skim milk concentration is 100g / L, 150g / L, 200g / L, 250g / L freeze-drying protective agent solution of different concentration gradients, according to the process of embodiment 1, carry out the expansion culture, centrifugation and washing of Lactobacillus plantarum CGMCC 5494 Compared with freeze-drying and viable count, the effect of the concentration of skim milk on the freeze-drying activity of Lactobacillus plantarum CGMCC 5494 was investigated. The results are attached Figure 4 Shown:

[0063] pass Figure 4 It can be seen that with the increase of the concentration of skim milk, the protective effect is significantly improved. However, when the concentration of skim milk exceeds 150g / L, the concentration has no significant effect on the protective effect. The protective effects of 150g / L, 200g / L, and ...

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Abstract

The invention discloses a dietary supplement of probiotics and plant extracts capable of alleviating lead toxicity, belonging to the technical field of food. The present invention uses the bacteria powder obtained by freeze-drying the strain as the main drug, supplemented with plant extracts, and then adds suitable auxiliary materials to prepare a dietary supplement of probiotics and plant extracts in the form of tablets by direct powder compression method. It contains live bacteria content greater than 10 9 CFU / g of Lactobacillus plantarum CGMCC 5494. The dietary supplement obtained by this method can reduce the lead content in lead-exposed mice; restore the oxidative stress damage caused by lead exposure; increase the activity of aminolevulinic acid dehydratase in lead-exposed mice; reduce the zinc content in lead-exposed mice. Protoporphyrin content; restoration of mental impairment in lead-exposed mice. Has broad market prospects.

Description

technical field [0001] The invention relates to a dietary supplement of probiotics and plant extracts capable of alleviating lead toxicity, and belongs to the technical field of food. Background technique [0002] Lead is a silvery-white heavy metal with a bluish tint, which is easily oxidized and darkens in the air. Lead is widely used, it can effectively block X-rays and radioactive rays, and it is also the main material for making batteries. In addition, lead compounds are raw materials for many pigments. Lead pollution refers to the pollution caused by lead or lead compounds to the surrounding environment, and its concentration and chemical form are directly related to its degree of harm. The strong enrichment of lead makes it difficult to degrade in the environment and cannot be reduced through the purification of the environment itself. If it exceeds the normal range, it will not only endanger human health, but also lead to deterioration of environmental quality. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/135A23L33/105A23L33/24A23P10/28
CPCA23V2002/00A23V2400/169A23V2250/214A23V2200/30A23V2250/21166A23V2250/2132A23V2250/51084A23V2250/51086
Inventor 翟齐啸陈卫田丰伟刘洋赵建新王刚张秋香刘小鸣张灏
Owner JIANGNAN UNIV
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