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Multifunctional thermosensitive agarose hydrogel and preparation method and application thereof

A kind of agarose hydrogel, multi-functional technology, applied in the direction of medical formula, medical preparations of non-active ingredients, wave energy or particle radiation treatment materials, etc., can solve the problems of uneven distribution of DOX oil sol, limited tumor treatment, etc. Achieve superior light-to-heat conversion ability, simple process, and low raw material cost

Inactive Publication Date: 2016-08-31
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a multifunctional temperature-sensitive agarose hydrogel and its preparation method and application. To solve the PLGA / MoS in the prior art 2 / DOX oil sol is unevenly distributed in the tumor, a technical problem for limited tumor treatment

Method used

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  • Multifunctional thermosensitive agarose hydrogel and preparation method and application thereof
  • Multifunctional thermosensitive agarose hydrogel and preparation method and application thereof
  • Multifunctional thermosensitive agarose hydrogel and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0032] Dissolve 1.5 g of agarose in 98.5 mL of deionized water, place it in a water bath at 90°C and stir for 120 minutes to obtain an aqueous solution of agarose. Then, according to the final Mo concentration of 200, 100, 50 or 20 ppm 2 / Bi 2 S 3 -PEG nanosheet dispersion (refer to Adv. Mater., 2015, 27, 2775 for the synthesis method) and 10 mg of doxorubicin hydrochloride (DOX), dissolved / dispersed in the above agarose solution, so that the concentration of Mo in the resulting AMD mixed solution The distribution is 200, 100, 50 or 20 ppm and stirring is continued for 30 minutes. After the stirring is completed, the resulting mixed solution is cooled, that is, the multifunctional agarose / photothermal material / chemotherapy drug hydrogel.

Embodiment 2

[0034] A little of the AMD hydrogel prepared in Example 1 (Mo concentration is 200ppm) was taken respectively, and the morphology and components of the hydrogel were analyzed. Field emission scanning electron microscopy and elemental analysis of its samples were performed on a FEI Magellan 400 field emission scanning electron microscope. Using Japan Rigaku D / MAX2200PC X-ray diffractometer (scanning conditions: Cu Kα rays, wavelength 1.54Å, operating voltage 40 kV, current 40 mA, scanning range: 3 o ~70 o ) to analyze the sample components.

[0035] From figure 1 (a) and (b), it can be seen that Agar-MoS 2 / Bi 2 S 3 Both PEG and AMD hydrogels have rough surfaces and their discontinuous pore structures, which lay the foundation for further cell adhesion and drug release. Element distribution scan ( figure 1 (c) and figure 1 (d)) confirms that Mo and S elements are uniformly distributed throughout the hydrogel material, from further XRD results ( figure 1 (g)) MoS can a...

Embodiment 3

[0037] Take 5mL AMD solution (Mo concentration 200ppm, solution temperature 65°C) in a 5mL cryovial, and combine with FLIR E60 thermal imaging camera to detect the fluidity and gelation process of AMD solution during the temperature change. Use SNB-1 digital viscometer to record the viscosity change of AMD solution during the temperature change. After the gelation process was completed, a certain mass of AMD hydrogel was soaked in a pH=4.0 buffer solution at 37°C, and the mass change of AMD hydrogel during 20 days of soaking was recorded.

[0038] Such as figure 2 As shown in (a), the AMD solution can still flow freely and has good injectability when it is higher than 37 °C (42 °C), and the AMD solution has not yet solidified into a gel ( figure 2 (a) left and center, figure 2 (b)); below 37 °C, the AMD mixed solution completely solidifies into a hydrogel ( figure 2 (a) right). (c–d) Strength detection of AMD hydrogels: Regardless of increasing and decreasing the tempe...

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Abstract

The invention provides multifunctional thermosensitive agarose hydrogel, which comprises an agarose solution, wherein a photo-thermal material and a chemotherapeutic medicine are loaded in the agarose solution; and the photo-thermal conversion material is any one of a two-dimensional MoS2 nanosheet, an MoS2 / Bi2S3-PEG nanosheet, graphene oxide or a palladium nanosheet. The invention further provides a preparation method of the hydrogel. The method comprises the following steps: dissolving agarose into a solvent, and heating and stirring the agarose until the agarose is completely dissolved; dispersing the two-dimensional photo-thermal conversion material and the chemotherapeutic medicine into the agarose solution to obtain a mixed solution; cooling the mixed solution to obtain the multifunctional thermosensitive agarose hydrogel. The invention further provides an application of the hydrogel as a carrier for loading an anti-tumor medicine or the photo-thermal conversion material. The multifunctional thermosensitive agarose hydrogel has excellent photo-thermal conversion capability, computerized tomography imaging capability and photoacoustic imaging capability, and can be applied to the field of efficient coordination treatment of tumors; and release of the chemotherapeutic medicine can be effectively controlled.

Description

technical field [0001] The invention belongs to the field of biological nanomaterials, and relates to a liposugar hydrogel, in particular to a multifunctional temperature-sensitive agarose hydrogel and its preparation method and application. Background technique [0002] Photothermal therapy of tumor is a novel minimal / non-invasive treatment method, which utilizes near-infrared (NIR) laser light with good tissue penetration ability to irradiate photothermal materials gathered near the tumor tissue, which absorbs NIR laser light and conducts Photothermal conversion kills cancer cells. At present, the research on photothermal therapy of tumors is mainly through the tail vein or intratumoral injection of photothermal material dispersion into model animals. The photothermal material is enriched at the tumor site. Most of the materials injected into the tail vein will be captured by organs of the reticuloendothelial system such as the liver and spleen, resulting in poor therape...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K31/704A61K31/4745A61K47/36A61P35/00
CPCA61K9/0019A61K9/0024A61K9/06A61K31/4745A61K31/704A61K41/0052A61K47/36
Inventor 王世革胡飞黄明贤
Owner UNIV OF SHANGHAI FOR SCI & TECH
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