Bamboo parasitic fungus and application thereof in fermentation production of hypocrellin

A technology of hypocrellin and bamboo yellow bacteria, which is applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of many side reactions, high toxicity of reagents, and many reaction steps, so as to achieve huge economic benefits and reduce environmental pollution , the effect of high metabolic activity

Active Publication Date: 2016-09-07
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional production method of hypocrellin is generally divided into biosynthesis method and chemical synthesis method. The biosynthesis method mainly uses corn flour, bran, wheat and other food crops as raw materials, and uses bamboo yellow fungus to ferment hypocrellin. After separation and purification, although the output has increased compared with other production methods, in the era of rising food prices, this method also makes the cost of the final product much higher, which is not conducive to the large-scale application of hypocrellin
The other is a chemical synthesis method, using 3,5-dihydroxybenzoic acid as the initial reactant, and through 12 steps of chemical reactions, hypocrellin is synthesized. However, this method has many and complicated reaction steps and many side reactions. The reagent is highly toxic and costly, so it is not a good way to produce hypocrellin
[0004] In 2005, China's corn stalks reached about 20207×10 4 t, and the proportion of straw returning to the field is very low. Although the country explicitly prohibits the burning of straw, it still cannot completely solve the problem of excessive straw.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 bamboo yellow ( Shiraia bambusicola ) Mutagenesis screening of NJTECH-1

[0029] Three strains of Phyllostachys japonica were collected from Huangshan in Anhui, Quzhou in Zhejiang and Yibin in Sichuan respectively. They were cultured in liquid potato juice medium and cultured at 26°C for five days, and the biomass and bamboo were measured at the same time. According to the amount of rhodomycin secretion, a strain with the most vigorous activity was collected from Yibin, Sichuan, named Super-3, as the dominant strain.

[0030] Wash the spores of Super-3 with normal saline, inoculate them in liquid seed medium, and incubate them at 26°C for 12 hours. Dilute the cultured spore suspension of Bajaflagellate to 10 cells by 10-fold dilution method. 7 / mL, take 0.1 mL of the bacterial solution and evenly spread it on a sterile empty plate, and carry out N + ion beam implantation, N + The ion beam implant dose is (90, 135, 180, 225, 270)×2.6×10 13 N + / cm 2 , ...

Embodiment 2

[0031] Embodiment 2 bamboo yellow ( Shiraia bambusicola ) Culture and physiological characteristics of NJTECH-1

[0032] The bamboo yellow ( Shiraia bambusicola ) The culture conditions of NJTECH-1 are:

[0033] The strain adopts aerobic culture, and the carbon source used to cultivate the strain can be glucose, sucrose, fructose, lactose, soluble starch, rice, corn and other materials; the nitrogen source used to cultivate the strain can be beef extract, Peptone, yeast extract, sodium nitrate, ammonium sulfate and other materials; the optimum temperature range for the growth of the strain is 24-28°C, the pH range is 4-9, and the optimum pH range is 5-7. It can also be added during the strain cultivation process Dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, magnesium phosphate and other inorganic salts.

[0034] The bamboo yellow ( Shiraia bambusicola ) The physiological and morphological characteristics of NJTECH-1 are:

[0035] Plate...

Embodiment 3

[0036] Embodiment 3 bamboo yellow ( Shiraia bambusicola ) (LBR-SB6) fermented to produce Hypocretin A

[0037] Wash the spores of bamboo yellow fungus (LBR-SB6) (preserved by the Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University) with normal saline, put them into the liquid seed medium, cultivate them at 26°C, 120rpm for 40h, and take 5mL of the seed solution Access to solid medium, temperature control at 26 ℃ culture. The liquid seed culture medium includes the following components: glucose 2g / L, dipotassium hydrogen phosphate 0.1g / L, anhydrous magnesium sulfate 0.05g / L, beef extract 0.3g / L, pH natural.

[0038] The seed liquid is inserted into a solid medium for fermentation and cultivation at a temperature of 26°C to 30°C to produce hypocrellin. The solid medium includes the following components (%) in mass ratio: 60 mesh corn flour 68.9, bran 9.85, rice bran 20, glucose 1, KH 2 PO 4 0.2 ,MgSO 4 ·7H 2 O 0.05.

[0039] As a result...

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PUM

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Abstract

The invention discloses a bamboo parasitic fungus and application thereof in fermentation production of hypocrellin. Three bamboo parasitic fungi are separated from Shiraia bambusicola respectively collected from Huangshan region of Anhui province, Quzhou region of Zhejiang province and Yinbin region of Sichuan province, are cultured by virtue of a basal culture medium and is processed by virtue of ion beams, and therefore, a strain with the highest vigorous activity is obtained, is named as Shiraia bambusicola NJTECH-1 and has been preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC NO. 11617. The bamboo parasitic fungus has very high metabolic activity and is applied to the fermentation production of the hypocrellin, and the yield of the hypocrellin is high. The field waste straws and animal dung can be taken as fermentation substrates, so that the quantity of incinerated straws can be reduced, and the environmental pollution can be reduced while the cost is saved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a bamboo yellow fungus and its application in fermenting and producing hypocrellin. Background technique [0002] Bamboo yellow is mainly distributed in some provinces in the southeast of China. In addition, it is also distributed in Japan in Asia, but it has not been reported in other countries. Hypocretin produced by bamboo yellow fungus has good effects of dispersing stasis and promoting blood circulation, dispelling wind and dampness, invigorating vital energy, promoting metabolism, and enhancing physical fitness. It is mostly used clinically to treat gastric pain, rheumatoid arthritis, Traumatic injuries and sciatica and other diseases. Moreover, this pigment is safe and non-toxic, bright red in color, strong in coloring power and rich in health care functions, and has broad prospects as a food pigment. [0003] The traditional production method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/26C12R1/645
CPCC12P7/26C12N1/145C12R2001/645
Inventor 郝宁胡洁丁叶刘兆星许琳欧阳平凯
Owner NANJING UNIV OF TECH
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