Bamboo parasitic fungus and application thereof in fermentation production of hypocrellin
A technology of hypocrellin and bamboo yellow bacteria, which is applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of many side reactions, high toxicity of reagents, and many reaction steps, so as to achieve huge economic benefits and reduce environmental pollution , the effect of high metabolic activity
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Embodiment 1
[0028] Embodiment 1 bamboo yellow ( Shiraia bambusicola ) Mutagenesis screening of NJTECH-1
[0029] Three strains of Phyllostachys japonica were collected from Huangshan in Anhui, Quzhou in Zhejiang and Yibin in Sichuan respectively. They were cultured in liquid potato juice medium and cultured at 26°C for five days, and the biomass and bamboo were measured at the same time. According to the amount of rhodomycin secretion, a strain with the most vigorous activity was collected from Yibin, Sichuan, named Super-3, as the dominant strain.
[0030] Wash the spores of Super-3 with normal saline, inoculate them in liquid seed medium, and incubate them at 26°C for 12 hours. Dilute the cultured spore suspension of Bajaflagellate to 10 cells by 10-fold dilution method. 7 / mL, take 0.1 mL of the bacterial solution and evenly spread it on a sterile empty plate, and carry out N + ion beam implantation, N + The ion beam implant dose is (90, 135, 180, 225, 270)×2.6×10 13 N + / cm 2 , ...
Embodiment 2
[0031] Embodiment 2 bamboo yellow ( Shiraia bambusicola ) Culture and physiological characteristics of NJTECH-1
[0032] The bamboo yellow ( Shiraia bambusicola ) The culture conditions of NJTECH-1 are:
[0033] The strain adopts aerobic culture, and the carbon source used to cultivate the strain can be glucose, sucrose, fructose, lactose, soluble starch, rice, corn and other materials; the nitrogen source used to cultivate the strain can be beef extract, Peptone, yeast extract, sodium nitrate, ammonium sulfate and other materials; the optimum temperature range for the growth of the strain is 24-28°C, the pH range is 4-9, and the optimum pH range is 5-7. It can also be added during the strain cultivation process Dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, magnesium phosphate and other inorganic salts.
[0034] The bamboo yellow ( Shiraia bambusicola ) The physiological and morphological characteristics of NJTECH-1 are:
[0035] Plate...
Embodiment 3
[0036] Embodiment 3 bamboo yellow ( Shiraia bambusicola ) (LBR-SB6) fermented to produce Hypocretin A
[0037] Wash the spores of bamboo yellow fungus (LBR-SB6) (preserved by the Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University) with normal saline, put them into the liquid seed medium, cultivate them at 26°C, 120rpm for 40h, and take 5mL of the seed solution Access to solid medium, temperature control at 26 ℃ culture. The liquid seed culture medium includes the following components: glucose 2g / L, dipotassium hydrogen phosphate 0.1g / L, anhydrous magnesium sulfate 0.05g / L, beef extract 0.3g / L, pH natural.
[0038] The seed liquid is inserted into a solid medium for fermentation and cultivation at a temperature of 26°C to 30°C to produce hypocrellin. The solid medium includes the following components (%) in mass ratio: 60 mesh corn flour 68.9, bran 9.85, rice bran 20, glucose 1, KH 2 PO 4 0.2 ,MgSO 4 ·7H 2 O 0.05.
[0039] As a result...
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