Culture medium and preparation method for selective separation culture extensively drug-resistant pseudomonas aeruginosa

Abstract

The invention discloses a culture medium and a preparation method for selective separation culture extensively drug-resistant pseudomonas aeruginosa. Culture supernatant fluid and an antibiotic combination for pseudomonas aeruginosa in a logarithmic phase are added into an NAC basal culture medium. The culture supernatant fluid contains quorum sensing substances secreted by pseudomonas aeruginosa, the quorum sensing substances can accelerate growth of bacteria and promote secretion of aeruginosa pigments, and the detection time is greatly shortened. The antibiotic combination comprises carbapenems antibiotics, aminoglycoside antibiotics and quinolone antibiotics, the additive amount of the carbapenems antibiotics is 0.016 g / L, the additive amount of the aminoglycoside antibiotics is 0.016 g / L, and the additive amount of the quinolone antibiotics is 0.016 g / L, the antibiotic combination can specifically inhibit growth of sensitive pseudomonas aeruginosa strains and growth of single-drug resistant pseudomonas aeruginosa strains, the purpose of selective separation culture of extensively drug-resistant pseudomonas aeruginosa is achieved, and specificity and sensitivity are high. Moreover, the culture medium can be stored for a long time and can be effectively applied to quick detection of extensively drug-resistant pseudomonas aeruginosa in clinical samples.

Description

technical field [0001] The invention belongs to the field of pathogenic microbiology, and in particular relates to a culture medium for selectively isolating and cultivating extensively drug-resistant Pseudomonas aeruginosa and a preparation method thereof. Background technique [0002] Pseudomonas aeruginosa is a common opportunistic pathogen that can cause purulent lesions. After infection, the pus and exudate are green, so it is also known as Pseudomonas aeruginosa. It is one of the clinically important opportunistic pathogens. one. Pseudomonas aeruginosa infection can occur in any part and tissue of the human body, usually in burns or trauma areas, middle ear, cornea, urethra and respiratory tract, and can also cause endocarditis, gastroenteritis, empyema and even sepsis, and even lead to Severe respiratory infections, such as those in patients with chronic fibrosis of the lungs. Pseudomonas aeruginosa infection is difficult to treat due to high drug resistance and bio...

AI Technical Summary

Problems solved by technology

N-acylhomoserine lactones (N-acylhomoserine lactones, AHLs) are the quorum-sensing substances of Gram-negative bacteria that have been studied more at present, which can promote the secretion of pyocyanin by Pseudomonas aeruginosa earlier. The molecular weight of the sensing substance is very close, so it is difficult to separate and purify, and the cost of purification is relatively high

In addition, the quorum-sensing substances of bacteria are extremely complex. Artificially adding a single substance to the culture medium will have different effects under different conditions.

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