Simple and efficient preparation method of nano calcium phosphate

A nano-calcium phosphate, high-efficiency technology, applied in the field of biomedical materials, can solve the problems of difficult control of the nucleation and growth of calcium phosphate crystals, affect the yield of nano-calcium phosphate, and increase the production cost, so as to improve biocompatibility and benefit The effect of large-scale production and simple preparation method

Inactive Publication Date: 2016-10-12
SOUTHWEST JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Calcium phosphate crystal nucleation and growth is difficult to control due to its fast precipitation rate
Although it can be reduced by reducing Ca 2+ and PO 4 3- The method of reaction concentration and temperature reduces the reaction rate to better control its crystal nucleation and growth; but reducing the concentration and temperature will affect the yield of nano-calcium phosphate
The growth of nano-calcium phosphate crystals can also be controlled by adding surfactants, but the added surfactants may cause biocompatibility problems
In addition, the precipitation of calcium phosphate synthesized by wet method can easily lead to the formation of chemically bonded hard aggregates between particles during the drying process (Wang Midang, Li Mei, Liu Zhaogang, etc. Agglomeration mechanism, characterization and elimination of ultrafine powders. China Powder Body Technology, 2008,14(3):46-51); By adopting freeze-drying and supercritical drying methods, hard agglomeration can be avoided, which leads to a significant increase in its preparation cost

Method used

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  • Simple and efficient preparation method of nano calcium phosphate
  • Simple and efficient preparation method of nano calcium phosphate
  • Simple and efficient preparation method of nano calcium phosphate

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Experimental program
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Effect test

Embodiment 1

[0036] A kind of simple and efficient preparation method of nanometer calcium phosphate, its steps are:

[0037] A. Preparation of calcium salt solution and phosphate solution:

[0038] Dissolve calcium nitrate tetrahydrate in deionized water to obtain calcium salt solution; ammonium dihydrogen phosphate (NH 4 h 2 PO 4 ) is dissolved in deionized water to obtain a phosphate solution;

[0039] B. Wet Synthesis of Nano Calcium Phosphate:

[0040] Under the action of magnetic stirring, add neridronate sodium into the calcium salt solution to dissolve neridronate sodium in the calcium salt solution; Reaction system, at the same time through the pH regulator - 0.1mol / L NH 4 OH solution, so that the pH value of the reaction system is 7.0; after the titration is completed, the mol ratio of calcium, neridronic acid sodium, and phosphorus in the reaction system is 0.5:0.01:1;

[0041] After the titration is completed, continue to stir for 30 minutes, and pass the pH regulator—0.1mo...

Embodiment 2

[0045] A kind of simple and efficient preparation method of nanometer calcium phosphate, its steps are:

[0046] A. Preparation of calcium salt solution and phosphate solution:

[0047] Dissolve calcium nitrate tetrahydrate in deionized water to obtain calcium salt solution; ammonium dihydrogen phosphate (NH 4 h 2 PO 4 ) is dissolved in deionized water to obtain a phosphate solution;

[0048] B. Wet Synthesis of Nano Calcium Phosphate:

[0049] Under the action of magnetic stirring, sodium opadronate is added to the calcium salt solution to dissolve sodium opadronate in the calcium salt solution; then, at a temperature of 37°C, the phosphate solution is dropped into the calcium salt solution to form Reaction system, at the same time through the pH regulator - 0.1mol / L NH 4 OH solution, so that the pH value of the reaction system is 8.0; after the titration is completed, the molar ratio of calcium, opadronate sodium, and phosphorus in the reaction system is 1:0.01:1;

[0...

Embodiment 3

[0054] A kind of simple and efficient preparation method of nanometer calcium phosphate, its steps are:

[0055] A. Preparation of calcium salt solution and phosphate solution:

[0056] Calcium nitrate tetrahydrate was dissolved in deionized water to obtain a calcium salt solution; disodium hydrogen phosphate (Na 2 HPO 4 ) is dissolved in deionized water to obtain a phosphate solution;

[0057] B. Wet Synthesis of Nano Calcium Phosphate:

[0058] Under the action of magnetic stirring, add alendronate sodium to the calcium salt solution to dissolve alendronate sodium in the calcium salt solution; then, at a temperature of 37°C, drop the phosphate solution into the calcium salt solution to form Reaction system, at the same time through the pH regulator - 1.0mol / L NH 4 OH solution, so that the pH value of the reaction system is 11.0; after the titration is completed, the molar ratio of calcium, sodium alendronate, and phosphorus in the reaction system is 1.67:0.02:1;

[0059...

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Abstract

A simple and efficient preparation method of nano calcium phosphate comprises steps as follows: A, a calcium salt solution and a phosphate solution are prepared; B, diphosphonate is dissolved in the calcium salt solution, the phosphate solution is dropwise added to the calcium salt solution at the temperature of 37 DEG C, a reaction system is formed, and the pH value of the reaction system is adjusted to 7.0-11.0; the mole ratio of calcium to diphosphonate to phosphorus in the reaction system is (0.50-1.67): (0.01-0.02):1; after titration is completed, the reaction system is continuously stirred for 1-120 min, and the pH value of the reaction system is adjusted to 7.0-11.0; centrifugal washing and filtration are performed, precipitates are re-suspended in absolute ethyl alcohol, and a turbid liquid is obtained; C, a diphosphonate chemical is added to the turbid liquid, nano calcium phosphate slurry is obtained, and the mole ratio of diphosphonate to phosphorus in the nano calcium phosphate slurry is (0.01-0.02):1; the nano calcium phosphate slurry is subjected to ball milling treatment at the rotating speed of 300-600 r/min for 4-12 h and finally dried, and nano calcium phosphate powder is obtained.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a simple and efficient method for preparing nanometer calcium phosphate. Background technique [0002] Calcium phosphate is the main inorganic component of bones and teeth. Studies have found that the ultrastructure of bones and teeth is composed of nano-calcium phosphate, and nano-calcium phosphate has many special physical and chemical properties such as surface effect, small size effect and high reactivity. , nano-calcium phosphate has attracted widespread attention. At present, nano-calcium phosphate is mainly used as a drug carrier for tumor-targeted therapy and a gene carrier for disease diagnosis, and can also be used for the preparation of calcium phosphate artificial bone scaffolds. The constructed calcium phosphate scaffold has high mechanical strength and excellent biological performance. Since the construction of calcium phosphate scaffolds ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B25/32
CPCC01B25/325C01P2002/72C01P2002/85C01P2004/03C01P2004/04C01P2004/16
Inventor 屈树新高雪玲代春初刘玮玮肖雯
Owner SOUTHWEST JIAOTONG UNIV
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