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Method for extracting stevioside from stevia rebaudiana leaves

A technology of stevia leaves and steviol glycosides, applied in the field of steviol glycoside extraction, can solve problems such as low extraction rate, and achieve the effects of improving purity, best flocculation effect, and improving purity and whiteness

Inactive Publication Date: 2016-10-26
BENGBU HUADONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many traditional extraction processes of steviol glycosides in industrial production, including hot water extraction, dialysis, enzymatic method, supercritical CO 2 Extraction method, ultra-high pressure method, reflux sleeve extraction method, countercurrent method, etc., but these methods often have the defect of low extraction rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The extraction method of steviol glycosides in a kind of stevia leaf that the present invention proposes comprises the following steps:

[0026] S1. Rinse the stevia leaves with clean water, then soak them in absolute ethanol for 0.5h, take them out, dry them until the water content is 6wt%, grind them and sieve them to obtain stevia leaf powder;

[0027] S2. Mix stevia leaf powder and water evenly in a mass ratio of 1:5, then add compound enzyme, wherein the quality of the compound enzyme is 0.25% of the quality of stevia leaf powder, adjust the pH value to 3, and then microwave Heat to 45°C and keep stirring for 5 minutes, then sonicate for 50 minutes, and obtain the extract and filter residue after filtration;

[0028] S3, filter residue and diethyl ether are added in the centrifuge in the ratio of 1:3 by mass ratio, carry out centrifugation after stirring evenly, wherein, the speed of centrifugation is 3500r / min, the time of centrifugation is 50min, after standing f...

Embodiment 2

[0033] The extraction method of steviol glycosides in a kind of stevia leaf that the present invention proposes comprises the following steps:

[0034] S1. Rinse the stevia leaves with clean water, then soak them in absolute ethanol for 1.5 hours, take them out, dry them to a moisture content of 5 wt%, crush them, and sieve them to obtain stevia leaf powder;

[0035] S2, mix the stevia leaf powder and water evenly in a mass ratio of 1:10, then add the compound enzyme, wherein the quality of the compound enzyme is 0.2% of the quality of the stevia leaf powder, adjust the pH value to 4, and then microwave Heat to 35°C and keep stirring for 10 minutes, then sonicate for 20 minutes, and obtain the extract and filter residue after filtration;

[0036]S3, filter residue and ether are added in the centrifuge in the ratio of 1:5 by mass ratio, carry out centrifugation after stirring evenly, wherein, the speed of centrifugation is 2500r / min, the time of centrifugation is 100min, after ...

Embodiment 3

[0041] The extraction method of steviol glycosides in a kind of stevia leaf that the present invention proposes comprises the following steps:

[0042] S1. Rinse the stevia leaves with clean water, then soak them in absolute ethanol for 0.8h, take them out, and dry them at 60°C until the moisture content is 6wt%. After crushing, pass through a 50-mesh sieve to obtain stevia leaf powder;

[0043] S2, Stevia rebaudiana leaf powder and water are mixed uniformly in the ratio of 1:6 by mass ratio, then add compound enzyme, wherein, described compound enzyme is the mixture of cellulase and pectinase by weight ratio of 5:6, The quality of the compound enzyme is 0.24% of the mass of the stevia leaf powder, adjust the pH value to 3.2, and then microwave heating to 43°C for 6.5 minutes while insulated and stirring, wherein, during the microwave heating process, the power of the microwave is 580W, and then ultrasonic for 40min, wherein , the ultrasonic power is 120W, and the extract and ...

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PUM

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Abstract

The invention discloses a method for extracting stevioside from stevia rebaudiana leaves. The method comprises the following steps of washing, soaking, drying stevia rebaudiana leaves and sieving to obtain stevia rebaudiana leaf powder; mixing the stevia rebaudiana leaf powder with water, adding complex enzyme, adjusting the pH value and heating by microwave, extracting and filtering by ultrasound to obtain an extracting solution and a filter residue; mixing the filter residue with diethyl ether, performing centrifugal separation to obtain a total extract; mixing the total extract with a ferric trichloride solution and a calcium hydroxide solution; after performing heating, heat preservation and cooling, mixing with a sodium hydroxide solution; after adjusting the pH value, mixing with an aluminum sulfate solution, after stirring, performing supercritical extraction to obtain an extract liquor; using AB-8 type macroporous resin to adsorb the extract liquor; performing elution, distillation, concentration and drying, so as to obtain a stevioside crude product; putting the stevioside crude product into water; ultrasonically dissolving and adding active aluminium oxide for adsorbing; and then filtering, washing, subsiding, combining filter liquor and washing liquor, and concentrating and drying.

Description

technical field [0001] The invention relates to the technical field of stevioside extraction, in particular to a method for extracting stevioside from stevia leaves. Background technique [0002] Stevia is a small evergreen shrub native to the South American Paraguayan Plateau between 25-26 degrees south latitude, and has been introduced all over the world. Its leaves contain a class of steviol glycosides, which are derived from a class of tetracyclic diterpenes. It is mainly composed of 8 kinds of glycosides including stevioside, stevioside A, stevioside B and stevioside C, and its sweetness is about 300 times that of sucrose. Studies have shown that stevioside has the characteristics of high sweetness, low calorie, and no obvious side effects. It can inhibit high blood sugar and high blood pressure, and has anti-inflammatory, anti-tumor, antidiarrheal and diuretic effects, and assists in immune regulation. Currently, South America, Japan and China have widely used steviol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256Y02P20/54
Inventor 邢飞邢伟张志华庞正银
Owner BENGBU HUADONG BIOLOGICAL TECH
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