A method for producing 2,3-butanediol by solid-state fermentation of Bacillus polymyxa
A technology of Bacillus polymyxa and solid-state fermentation, which is applied in sterilization methods, microorganism-based methods, solid-phase fermentation bioreactors, etc., to achieve the effects of easy availability of components, convenient construction and simple structure
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Embodiment 1
[0043] Example 1: A fermentation device built to simulate shallow-pan solid-state fermentation
[0044] The apparatus for simulating shallow plate solid-state fermentation of the present invention mainly includes a large Buchner funnel 1 with a diameter of 250 mm, a rubber ring 2, and a suction filter bottle 3 from top to bottom, and a filter tip is provided on one side of the suction filter bottle 3 in the middle and upper part. 4. The filter tip 4 communicates with the aeration pump 6 through the rubber tube 5, and an air filter 7 is provided between the filter tip 4 and the rubber tube 5. The Buchner funnel 1 is the main fermentation unit, and its upper end is wrapped with gauze 8 Sealing; a perforated interlayer 9 is set in the middle of the Buchner funnel 1, and a six-layer thick wet gauze 10 is tiled on the perforated interlayer 9, and the configured solid medium 11 is evenly placed on the wet gauze 10.
[0045] Method of use: first spread a six-layer thick wet gauze 10 ...
Embodiment 2
[0046] Embodiment 2: the fermentation process in the Erlenmeyer flask of Jerusalem artichoke dregs
[0047] 1) Take the Jerusalem artichoke dry powder raw material that has been crushed through a 60-mesh sieve, and mix it thoroughly under the condition of a solid-to-liquid mass ratio of 1:6. After stirring and reacting in a water bath at 75°C for 2 hours, after cooling down to room temperature, mix it with Ca(OH) 2 The pH value of the mixture was adjusted to 9.0, and then leached in a water bath at 85° C. for 1 hour, and the solid and liquid were separated to obtain a crude inulin extract containing rich inulin and Jerusalem artichoke dregs containing part of inulin.
[0048] 2) Add organic nitrogen source yeast powder (mass ratio 1%) and mixed nutrient salt solution (containing MgSO 4 ·7H 2 O 0.05g, K 2 HPO 4 0.309g, MnSO 4 ·H 2 O0.0001g, NH 4 Cl 0.093g, FeSO 4 ·7H 2 O 0.004g, ZnSO 4 ·7H 2 (0.0001g), the solid-to-liquid ratio is 1:4, and the pH value is 7, fully st...
Embodiment 3
[0054] Example 3: Solid-state fermentation of Jerusalem artichoke dregs simulated in a shallow pan
[0055] Referring to Example 2, the difference is that the fermentation equipment used is the simulated shallow plate solid-state fermentation device built in Example 1, and the specific implementation method is to spread the solid-state fermentation medium substrate prepared in Example 1 on the Brinell plate with a uniform thickness. In the funnel, then seal the upper end of the Buchner funnel with gauze, seal the interface between the lower end of the Buchner funnel and the suction filter bottle with a rubber ring, sterilize by high-pressure steam at 121°C for 30min, and after cooling, inoculate more Myxobacillus seed liquid, and then, place the whole simulated flat plate solid-state fermentation device in a constant temperature and humidity box, connect the air filter and the aeration pump with a rubber tube at the filter tip of the suction filter bottle, and supply the air to...
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