Method for preparing biodiesel by adopting magnetic carrier immobilized lipase

Abstract

The invention discloses a method for preparing biodiesel by adopting magnetic carrier immobilized lipase. According to the method, the immobilized lipase magnetic nanoparticle catalyst is adopted for catalyzing the reaction, wherein the immobilized lipase magnetic nanoparticle catalyst is prepared by carrying out immobilization on lipase-producing bacteria by adopting magnetic nanoparticles as a carrier and by adopting a covalent cross-linking method. The technical scheme has the advantages that the technology is simple and feasible, the requirements on devices are low, the related catalyst is high in transesterification efficiency, in addition, the immobilized enzyme has high enzyme activity, is stable, is convenient to recycle and separate, and can be recycled, and moreover, strong acids and strong alkalis are not used, so that the pollution to the environment is reduced.

Description

technical field [0001] The invention belongs to the fields of bioengineering and energy chemical industry, and in particular relates to a method for preparing biodiesel catalyzed by immobilized lipase with magnetic nanoparticles. Background technique [0002] With economic development and social progress, the consumption of fossil energy, especially petroleum energy, is growing rapidly. The development of new bio-energy as a substitute for petrochemical diesel can promote the development of new energy and ensure energy security and national security. Biodiesel not only has combustion characteristics similar to petrochemical diesel, but also has excellent characteristics such as environmental friendliness, high safety, good lubricity, renewable and biodegradable. [0003] Among the production processes of biodiesel, the chemical base catalysis method has achieved industrial production. Although the catalyst is relatively cheap, the reaction time is short, and the conversion r...

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Problems solved by technology

[0003] Among the production processes of biodiesel, the chemical base catalysis method has achieved industrial production. Although the catalyst is relatively cheap, the reaction time is short, and the conversion rate is high, there are still a series of inherent defects: (1) The quality requirements for raw materials are relatively high. High, low recycling rate of waste oil and industrial waste; (2) complex process, high energy consumption; (3) large amount of alcohol and difficult to recycle; (4) difficult to recycle the by-product glycerin, and difficult to separate the catalyst from the product , which increases the cost of follow-up treatment, and the discharge of waste lye forms a new source of environmental pollution

A large number of free enzymes cannot be used many times, recycling is difficult, and the price of lipase itself is very expensive, making the cost of enzymatically producing biodiesel very high, which cannot be used for industrial promotion and use

The loss of lipase by physical adsorption method is small, and the specificity of lipase is retained, but the stability of immobilized lipase is poor, and it is not easy to store; the loss of lipase by cross-linking method is relatively large, but the stability of immobilized lipase is better, 62.5% vitality retained after 3 months

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