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Silk fibroin/ cellulose 3D printing ink

A silk fibroin, 3D printing technology, applied in the fields of medical science, pharmaceutical formulations, prostheses, etc., can solve the problems of affecting the mechanical properties of the stent, affecting the structural integrity of the printed stent, affecting the accuracy of the stent, etc., and achieving good biocompatibility sexual effect

Active Publication Date: 2017-01-04
常州增材制造研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since 3D printing technology needs to make the bio-ink change from a fluid state to a solid state in a very short time, the initial viscosity of the ink will affect the structural integrity of the printed scaffold, which in turn affects the accuracy of the scaffold, and the composition of the ink will affect the accuracy of the scaffold. mechanical properties
However, the current 3D printing bioink is difficult to balance the structural accuracy and mechanical properties of the scaffold.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A silk fibroin / bacterial cellulose nano whisker 3D printing ink, composed of 40wt% silk fibroin, 4wt% bacterial cellulose nanowhiskers, 10wt% glycerol, 4wt% bioactive glass and Water composition, preparation steps are:

[0035] (1) With a concentration of 0.5wt% Na 2 CO 3 Degumming silkworm cocoons with an aqueous solution, and then dissolving it with a LiBr aqueous solution with a concentration of 9.0 mol / L, and obtaining water-soluble silk fibroin with a molecular weight of 65 kDa after dilution, centrifugation, suction filtration, dialysis, and concentration;

[0036] (2) Boil the bacterial cellulose membrane with 0.5% sodium hydroxide aqueous solution to remove the bacteria and residual culture medium in the bacterial cellulose membrane, then rinse it repeatedly with deionized water until neutral, and treat it with alkali with a homogenizer The processed bacterial cellulose suspension was made into slurry, centrifuged to obtain bacterial cellulose homogenate, and ...

Embodiment 2

[0046] A tussah silk fibroin / bacterial cellulose nanofiber 3D printing ink, composed of 5wt% tussah silk fibroin, 30wt% bacterial cellulose nanofiber, 1wt% ethanol and water, the preparation steps are:

[0047] (1) With a concentration of 0.5wt% Na 2 CO 3 The aqueous solution degummed tussah cocoons, and then dissolved it with a LiBr aqueous solution with a concentration of 9.0 mol / L, and obtained water-soluble tussah silk fibroin with a molecular weight of 50 kDa after dilution, centrifugation, suction filtration, dialysis, and concentration;

[0048] (2) Bacterial cellulose membrane is boiled with 0.5wt% sodium hydroxide aqueous solution to remove the thallus and residual medium in the bacterial cellulose membrane, then repeatedly rinse to neutrality with deionized water, and alkali The treated bacterial cellulose suspension was made into slurry, centrifuged to obtain bacterial cellulose homogenate, and finally freeze-dried to obtain bacterial cellulose nanofibers with an a...

Embodiment 3

[0052] A castor silk fibroin / mulberry cellulose microfiber 3D printing ink, composed of 14wt% castor silk fibroin, 25wt% mulberry cellulose microfibers, 3wt% ribitol, 8wt% bone morphogenic protein and water, the preparation steps are:

[0053] (1) With a concentration of 0.5wt% Na 2 CO 3 Degumming castor silkworm cocoons with aqueous solution, and then dissolving it with LiBr aqueous solution with a concentration of 9.0mol / L, after dilution, centrifugation, suction filtration, dialysis and concentration, water-soluble castor silk fibroin protein with a molecular weight of 80kDa is obtained;

[0054] (2) Dry the bast of mulberry tree, cut it into 10cm sections, wash away impurities with clear water, pretreatment with alkali boiling, degumming by high temperature alkali boiling, bleaching, and drying to obtain mulberry with an aspect ratio of 150 and a diameter of 4 μm. Hull cellulose microfibers;

[0055] (3) Dissolve castor silk fibroin in water, and then add mulberry cellu...

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Abstract

The invention relates to silk fibroin / cellulose 3D printing ink, which comprises the following main ingredients: water-soluble silk fibroin with good biocompatibility, non-water soluble cellulose micron / nanometer materials with good biocompatibility, non-toxic polyhydric alcohols capable of inducing the silk fibroin to carry out physical crosslinking, bioactive substance capable of accelerating tissues to regenerate, and water. The silk fibroin / cellulose 3D printing ink disclosed by the invention has high viscosity, dynamic viscosity is 1000-5000cP, curing molding speed is high, self-gelation time is 0.5-3min, printing conditions are moderate, and printing temperature is 25-37DEG C; an obtained printing bracket has high precision and good mechanical property, a resolution ratio is 50-300mum, and a modulus of compression is 10-50MPa. The bracket formed by the silk fibroin / cellulose 3D printing ink can be used for the field of the repairing and the reconstruction of various damaged tissues or organs of a human body.

Description

technical field [0001] The invention belongs to the field of biological 3D printing ink, and relates to a silk fibroin / cellulose 3D printing ink. Background technique [0002] At present, tens of millions of people around the world suffer from various forms of trauma every year, and millions of people lose function due to fibrosis of important organs during the recovery process of diseases, and hundreds of thousands of people are eager to undergo various organ transplants. As an indispensable clinical treatment, organ transplantation mainly relies on the treatment mode of autologous or allogeneic transplantation, which is inevitably limited by organ sources, ethics, and immune rejection of the body, making it difficult to meet the needs of clinical treatment. . According to statistical results, only about 20% of the patients waiting for organ transplantation can undergo normal organ transplantation, and the rest can only continue to wait or take other treatment methods. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/50A61L27/22A61L27/20
CPCA61L27/20A61L27/227A61L27/50A61L27/54A61L2400/12A61L2430/40C08L89/00C08L1/02
Inventor 张耀鹏黄利朱钰方邵惠丽胡学超
Owner 常州增材制造研究院有限公司
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