Preparation method of microcarrier

Abstract

The invention relates to the technical field of cell culture, in particular to a preparation method of a microcarrier. The preparation method of the microcarrier comprises the steps that a high-molecular polymer is dissolved in mixed solvent prepared from DMSO and NMP or pure NMP to obtain a high-molecular polymer solution; the high-molecular polymer solution is put into an electrostatic generator with a needle injection device, the high-molecular polymer solution is charged through high voltage static electricity, a liquid drop is formed at the tail end of a needle and drops into extracting solvent, and organic solvent in the liquid drop is quickly removed through a solvent extracting principle, so that the microcarrier is quickly prepared. According to the preparation method, the time needed by the microcarrier preparation process is shortened, and the advantages of being simple and efficient are achieved; the high-molecular polymer microcarriers with the uniform particle size can be continuously and controllably produced, and the purpose of precisely regulating and controlling the size and morphology of the microcarriers is achieved.

Description

Technical field [0001] The invention relates to the technical field of cell culture, in particular to a method for preparing microcarriers. Background technique [0002] Microcarrier cell culture technology is to inoculate cells on microspheres of a certain size, and achieve rapid, large-scale and high-quality expansion of cells in a three-dimensional culture environment to facilitate tissue engineering needs and stem cell therapy. With the progress of material synthesis technology, the use of poly(lactic-co-glycolic acid, PLGA), polylactic acid (polylactic acid, PLA) and other non-toxic, biocompatibility and high degradability The microcarriers prepared by molecular materials can not only realize the large-scale expansion of cells in vitro, but also can load cells to repair damaged tissues, which expands the application scope of cell microcarriers. [0003] There are many ways to prepare microcarriers, such as spray drying method, double emulsification method and microsphere sint...

AI Technical Summary

Problems solved by technology

Its disadvantages are: 1) it is impossible to precisely control the morphology and size of the microcarriers, and the microcarriers obtained are often of different sizes; 2) the method of solidification by solvent volatilization often takes a long time to remove the organic solvent, and the preparation cycle Too long, the production efficiency is low, cause the current product cost to be too high and expensive; 3) The traditional way of preparing microcarriers usually uses the larger organic solvents such as dichloromethane and chloroform, which are easy to remain and have certain effects on cells. Toxic effect

All these shortcomings limit the application of microcarriers in in vitro cell culture and tissue repair

[0006] In addition, the reason why commercialized microcarriers are expensive and are generally only used for experiments is that traditional preparation techniques cannot achieve rapid and continuous production. Traditional microcarrier preparation techniques usually have low output and low efficiency, which cannot meet the market demand.

Images