Preparation method of molecular imprinting solid phase extraction film with triphenylmethane molecule replacing malachite green, and application

Abstract

The invention relates to a preparation method of a molecular imprinting solid phase extraction film with a triphenylmethane molecule replacing malachite green, and application. The triphenylmethane molecule is adopted as a substituted template molecule for the malachite green for preparing the molecular imprinting film, so that the problems of high template molecule cost, leakage of the template molecule during use and the like during the preparation of a molecular imprinting polymer are solved, and meanwhile, the identification ability of a molecular imprinting is still kept. The method provided by the invention is stable in polymerization reaction without side reaction products, and the reaction is easy to control; the malachite green molecular imprinting film prepared through the method is stable in physicochemical property, high in selectivity and fast in mass transfer rate, not only can be combined with an instrument method (i.e. liquid chromatogram, liquid chromatogram-mass spectrometer, etc.), but also can be combined with a fast detection method (i.e. euzymelinked immunosorbent assay, colloidal gold immunization, a rapid detection kit method, etc.), and is suitable for separating, extracting and enriching the malachite green with trace concentration in a complex sample.

Description

technical field [0001] The invention belongs to the fields of chemical synthesis, analytical chemistry and biotechnology, and relates to the preparation and application of a molecularly imprinted solid-phase extraction membrane, in particular to a method for preparing a highly selective malachite green molecularly imprinted membrane based on a substitute template molecule, and the method thereof The prepared membrane is suitable for selective separation, extraction and enrichment of trace amounts of malachite green and its analogue recessive malachite green in water samples and animal-derived tissues. Background technique [0002] In a complete sample analysis process, 70%-80% of the pretreatment time is taken up, and more than 60% of the error of the analysis result comes from the pretreatment steps of the sample. The emergence of molecular imprinting technology has improved the disadvantages of poor selectivity of traditional solid phase extraction technology. It is a pret...

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the existing pretreatment technology, and propose to use a triphenylmethane molecule as a substitute template molecule for malachite green to prepare molecularly imprinted membranes, so as to solve the problem of high price of template molecules in the preparation of molecularly imprinted polymers. Problems such as leakage of template molecules during use, while still maintaining the recognition ability of molecular imprinting

The existing molecularly imprinted materials have the problems of too deep "embedding" of imprinted sites, slow adsorption of target molecules on molecularly imprinted polymers, and low efficiency. Glass fiber membranes with loose, porous, and fast percolation functions are used as substrates. To prepare molecularly imprinted polymer membranes, which solves the problems of low extraction efficiency and small extraction capacity

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