Preparation method of molecular imprinting solid phase extraction film with triphenylmethane molecule replacing malachite green, and application

A solid-phase extraction membrane and triphenylmethane technology, which is applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve problems such as high price of template molecules, leakage of template molecules, and small extraction capacity , to achieve the effect of stable physical and chemical properties, short equilibration time and large extraction capacity

Inactive Publication Date: 2017-02-22
张毅
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the existing pretreatment technology, and propose to use a triphenylmethane molecule as a substitute template molecule for malachite green to prepare molecularly imprinted membranes, so as to solve the problem of high price of template molecules in the preparation of molecularly imprinted polymers. Problems such as leakage of template molecules during use, while still maintaining the recognition ability of molecular imprinting
The existing molecularly imprinted materials have the problems of too deep "embedding" of imprinted sites, slow adsorption of target molecules on molecularly imprinted polymers, and low efficiency. Glass fiber membranes with loose, porous, and fast percolation functions are used as substrates. To prepare molecularly imprinted polymer membranes, which solves the problems of low extraction efficiency and small extraction capacity

Method used

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  • Preparation method of molecular imprinting solid phase extraction film with triphenylmethane molecule replacing malachite green, and application
  • Preparation method of molecular imprinting solid phase extraction film with triphenylmethane molecule replacing malachite green, and application
  • Preparation method of molecular imprinting solid phase extraction film with triphenylmethane molecule replacing malachite green, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation and Application of Molecularly Imprinted Solid-Phase Extraction Membrane (MIP)

[0038] S01: Prepare a mixed organic solution containing triphenylmethane, 4-vinylbenzoic acid, crosslinking agent ethylene glycol dimethacrylate, and azo initiator azobisisobutyronitrile: mix 1mol of triphenylmethane, 4- 2 mol of vinyl benzoic acid, 6 mol of ethylene glycol dimethacrylate as a crosslinking agent, an appropriate amount of toluene as an organic solvent, and 6 mol of azo initiator azobisisobutyronitrile were subjected to ultrasonic miscibility treatment for 30 minutes to obtain a mixed solution;

[0039] S02: Put the mixed organic solution in a refrigerator at 4°C for 6 hours for prepolymerization, take out the mixed organic solution after the prepolymerization is completed, and pour nitrogen gas into it for several minutes, then pour the mixed organic solution into a reaction mold, At the same time, glass fiber membranes matching the size of the mold ar...

Embodiment 2

[0042] Example 2 Preparation and Application of Molecularly Imprinted Solid-Phase Extraction Membrane (MIP)

[0043] S01: Prepare a mixed organic solution containing triphenylmethane, trifluoromethacrylic acid, crosslinking agent divinylbenzene, and azo initiator azobisisoheptanonitrile: mix 1 mol of triphenylmethane, 3 mol of trifluoromethacrylic acid, 12 mol of the crosslinking agent divinylbenzene, an appropriate amount of organic solvent chloroform, and 7 mol of the azo initiator azobisisoheptanonitrile were subjected to ultrasonic miscibility treatment for 30 minutes to obtain a mixed solution;

[0044] S02: Put the mixed organic solution in a refrigerator at 4°C for 6 hours for prepolymerization, take out the mixed organic solution after the prepolymerization is completed, and pour nitrogen gas into it for several minutes, then pour the mixed organic solution into a reaction mold, At the same time, glass fiber membranes matching the size of the mold are added, and polyme...

Embodiment 3

[0047] Example 3 Preparation and Application of Molecularly Imprinted Solid-Phase Extraction Membrane (MIP)

[0048] S01: Prepare a mixed organic solution containing phenolphthalein, acrylamide, cross-linking agent ethylene glycol dimethacrylate, azo initiator dimethyl azobisisobutyrate: mix 1 mol of phenolphthalein, 3 mol of acrylamide, cross-linking agent 12 mol of ethylene glycol dimethacrylate, an appropriate amount of acetonitrile as an organic solvent, and 7 mol of azo initiator dimethyl azobisisobutyrate were subjected to ultrasonic miscibility treatment for 30 minutes to obtain a mixed solution;

[0049] S02: Put the mixed organic solution in a refrigerator at 4°C for 6 hours for prepolymerization, take out the mixed organic solution after the prepolymerization is completed, and pour nitrogen gas into it for several minutes, then pour the mixed organic solution into a reaction mold, At the same time, glass fiber membranes matching the size of the mold are added, and po...

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Abstract

The invention relates to a preparation method of a molecular imprinting solid phase extraction film with a triphenylmethane molecule replacing malachite green, and application. The triphenylmethane molecule is adopted as a substituted template molecule for the malachite green for preparing the molecular imprinting film, so that the problems of high template molecule cost, leakage of the template molecule during use and the like during the preparation of a molecular imprinting polymer are solved, and meanwhile, the identification ability of a molecular imprinting is still kept. The method provided by the invention is stable in polymerization reaction without side reaction products, and the reaction is easy to control; the malachite green molecular imprinting film prepared through the method is stable in physicochemical property, high in selectivity and fast in mass transfer rate, not only can be combined with an instrument method (i.e. liquid chromatogram, liquid chromatogram-mass spectrometer, etc.), but also can be combined with a fast detection method (i.e. euzymelinked immunosorbent assay, colloidal gold immunization, a rapid detection kit method, etc.), and is suitable for separating, extracting and enriching the malachite green with trace concentration in a complex sample.

Description

technical field [0001] The invention belongs to the fields of chemical synthesis, analytical chemistry and biotechnology, and relates to the preparation and application of a molecularly imprinted solid-phase extraction membrane, in particular to a method for preparing a highly selective malachite green molecularly imprinted membrane based on a substitute template molecule, and the method thereof The prepared membrane is suitable for selective separation, extraction and enrichment of trace amounts of malachite green and its analogue recessive malachite green in water samples and animal-derived tissues. Background technique [0002] In a complete sample analysis process, 70%-80% of the pretreatment time is taken up, and more than 60% of the error of the analysis result comes from the pretreatment steps of the sample. The emergence of molecular imprinting technology has improved the disadvantages of poor selectivity of traditional solid phase extraction technology. It is a pret...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F212/14C08F212/36C08F220/04C08F220/56C08F220/06C08J9/26B01J20/26B01J20/28B01J20/30
CPCB01J20/268B01J20/28033B01J20/3057C08F212/36C08F222/102C08F222/14C08J9/26C08F220/04C08F212/14C08F220/56C08F220/06
Inventor 张毅
Owner 张毅
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