Bovine respiratory syncytial cell virus antigen protein

A virus antigen, bovine respiratory technology, applied in the direction of virus, virus peptide, virus/bacteriophage, etc., can solve the problems of incomplete killing, disease, disease transmission, etc., and achieve control and prevention of losses, great economic significance and social significance Effect

Inactive Publication Date: 2017-03-22
烟台偌帝生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous advancement of human knowledge, the limitations of traditional vaccines are increasingly revealed: (1) animal and human viruses need to be cultured in animal cells, which makes the cost of vaccine production very high; (2) pathogenic viruses in vaccines The substance may not be completely killed or sufficiently attenuated during the vaccine production process, which will cause the vaccine to contain highly toxic pathogenic substances, which in turn will cause the disease to spread in a wider range; (3) Attenuated strains may occur mutation, disease-causing

Method used

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  • Bovine respiratory syncytial cell virus antigen protein
  • Bovine respiratory syncytial cell virus antigen protein
  • Bovine respiratory syncytial cell virus antigen protein

Examples

Experimental program
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Embodiment 1

[0027] Embodiment 1: SEQ 048055.1 is modified as follows to obtain a bovine respiratory syncytial virus antigenic protein of the present invention:

[0028] Bovine respiratory syncytial virus F-protein amino acid sequence SEQ 048055.1 Valine at position 148 is replaced by cysteine, isoleucine at position 288 is replaced by cysteine, and the two sites are connected by a disulfide bond, Carrying out corresponding protein synthesis, expression and purification, the obtained bovine respiratory syncytial virus antigen protein is referred to as RD-BRSV-DB1, and its amino acid sequence is referred to as SEQ DB1.

[0029] Bovine respiratory syncytial virus F-protein amino acid sequence SEQ 048055.1 Leucine at position 158 is replaced by cysteine, isoleucine at position 290 is replaced by cysteine, and the two sites are connected by a disulfide bond, Carrying out corresponding protein synthesis, expression and purification, the obtained bovine respiratory syncytial virus antigen protei...

Embodiment 2

[0048] Example 2: The specific process for preparing RD-BRSV-DB1-CF1-SC1-ID1 in Example 1. The protein structure and shape can be seen through the following three ways, which are x-ray Crystallography, nuclear magnetic resonance (NMR), and electron microscopy. The present invention adopts the X-ray method to observe the protein structure. Use the Solution maker 600 plux series to crystallize the target protein, use the X-ray system (SER-CAT22) to collect the crystal data of the crystallized target protein, and make it image, obtain the electronic cloud image of the protein structure, and obtain the key protein F-protein The data changes before and after infection, and the structural changes before and after infection are shown in Figure 2.

[0049] Perform cloud data processing on the above electronic cloud image, and use the corresponding software (Phenix) and wild protein sequence for data simulation to form a three-dimensional structural simulation graph of the protein stru...

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Abstract

The invention discloses a bovine respiratory syncytial cell virus antigen protein. Compared with SEQ 048055.1, an amino acid sequence of the bovine respiratory syncytial cell virus antigen protein is provided with at least two loci substituted by cysteines, the substitutive cysteines are connected through a disulfide bond, improvements such as internal disulfide bond, hole filling and single stranded connection are also performed on the basis, and meanwhile a preparation method of the antigen protein is provided. The antigen protein applies the technique and theory of protein crystallography, the structural change of the protein in virus infection and pathopoiesis process is determined, and then genetic engineering modification is conducted on the key virus protein according to a structural change result, so that the protein becomes a protein antigen only infecting without pathopoiesis and causing efficient reaction of animal body antibodies. The antigen protein can effectively prevent and treat bovine respiratory syncytial cell virus infection, an ideal viral vaccine can be developed on the basis, loss brought by virus inflection can be effectively controlled and prevented, and the antigen protein has great economic significance and social significance.

Description

technical field [0001] The invention belongs to the technical field of biological product preparation, and relates to a bovine respiratory syncytial virus antigen protein and a preparation method thereof. Background technique [0002] Bovine Respiration Syncytial Virus (BRSV) infection can cause bovine respiratory diseases, and is listed as an important bovine disease in European Community countries after bovine mucosal disease (BVD) and bovine infectious rhinotracheitis (IBR). one of the diseases. The disease is prevalent worldwide, and its harmfulness is that the incidence of BRSV infection reaches as high as 60%-80%, and the mortality rate reaches more than 20% in some outbreaks, causing great economic losses to the cattle industry. Although this BRSV has been included in the research and development category since the 1970s, ideal drugs and vaccines have not been developed due to various reasons. Although there have been reports on various inactivated, attenuated, reco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/135C07K1/30
CPCC07K14/005C12N2760/18522
Inventor 汤斌邱啟欲朱宁新赵斌
Owner 烟台偌帝生物工程有限公司
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