23 results about "Syncytial cell" patented technology

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The invention discloses a herbicide-resistant protein GAT hybridoma cell strain, an antibody generated by the herbicide-resistant protein GAT hybridoma cell strain and an application of the herbicide-resistant protein GAT hybridoma cell strain. The herbicide-resistant protein GAT hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46333. The preparation method comprises the following steps: a) purifying prokaryotic expression to obtain GAT recombinant protein; b) immunizing animals: immunizing BALB / c mice by taking the GAT recombinant protein as an antigen; c) cell fusion: splenocytes of the immune BALB / c mice are collected and fused with SP2 / 0 cells; and d) cell strain establishment: carrying out subcloning through a limited dilution method, carrying out ELISA detection after 5-7 days of subcloning until a hybridoma cell strain capable of stably secreting a positive antibody is screened out, and carrying out amplification, re-culture and storage. The monoclonal antibody secreted by the hybridoma cell strain lays a foundation for detection of herbicide-resistant protein GAT in transgenic crops, so that development, seed production and seed conservation of transgenic gat crops are supported, and stable industrial promotion of the transgenic gat crops and sustainable application of related transformants are guaranteed.

The invention discloses a herbicide-resistant protein GAT hybridoma cell strain, an antibody generated by the herbicide-resistant protein GAT hybridoma cell strain and application of the herbicide-resistant protein GAT hybridoma cell strain and the antibody. The herbicide-resistant protein GAT hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46332. The preparation method comprises the following steps: a) purifying prokaryotic expression to obtain GAT recombinant protein; b) immunizing animals: immunizing BALB / c mice by taking the GAT recombinant protein as an antigen; c) cell fusion: splenocytes of the immune BALB / c mice are collected and fused with SP2 / 0 cells; and d) cell strain establishment: carrying out subcloning through a limited dilution method, carrying out ELISA detection after 5-7 days of subcloning until a hybridoma cell strain capable of stably secreting a positive antibody is screened out, and carrying out amplification, re-culture and storage. The monoclonal antibody secreted by the hybridoma cell strain lays a foundation for detection of herbicide-resistant protein GAT in transgenic crops, so that development, seed production and seed conservation of transgenic gat crops are supported, and stable industrial promotion of the transgenic gat crops and sustainable application of related transformants are guaranteed.

The invention discloses an osteoclast culture method as well as a culture medium and application thereof, the culture method is based on primary monocyte extraction, inoculation is performed according to a specific density, then quantitative M-CSF is added, and culture is performed overnight; on the second day, when the cell confluence degree of the mononuclear bone marrow hematopoietic stem cells is 5-30%, supplementing quantitative RANKL into each hole, continuously culturing for 2-4 days, then changing the solution, and obtaining mature osteoclasts on the sixth-seventh day. According to the invention, the optimization relationship among the cell activity, the cell density and the culture medium in the primary culture process of the osteoclast is deeply studied, and from the perspective of cost reduction and efficiency improvement, the use cost of cell factors is remarkably reduced, the number of times of liquid change is reduced, and the traditional culture time of 10-12 days (calculated from cell extraction) is shortened to 6-7 days. The average diameter of the mature osteoclast cultured by the method is larger, the number of fused cell nucleuses is larger, the space proportion of the mature osteoclast is not less than 80%, and the cell quality is obviously higher than that in the prior art.

The application provides a tumor immune microenvironment multi-cell recognition and spatial analysis system, comprising: an intrinsic signal generation module generating an initial intrinsic signal vector; a preliminary functional marker module generating a preliminary phenotype classification label map based on spatial centroid coordinates of cell objects; a functional neighborhood construction module calculating and generating a functional neighborhood feature vector; a composite feature splicing module constructing a composite state feature vector; an effective function determination module decoding to obtain a continuous value feature vector output by a function determination model representing the final function strength under the regulation of the microenvironment; and a functional map rendering module rendering to generate a cell effective function map under the regulation of the environment. The application solves the problem that, in the prior art, when interpreting cell functions, the intrinsic state information of the cells and the local functional microenvironment context information cannot be effectively fused, resulting in deviation in the description of the spatial distribution map of tumor microenvironment function heterogeneity.

The invention belongs to the technical field of biological medicine, and discloses a nano antibody for resisting porcine delta coronavirus nucleocapsid protein as well as a recombinant, preparation and application of the nano antibody. According to the nano antibody for resisting the porcine delta coronavirus nucleocapsid protein and the recombinant nano antibody thereof, the specific nano antibody 62Nb targeting the PDCoV N protein is obtained through screening, efficient intracellular delivery of the nano antibody 62Nb is realized through engineering modification of a fusion cell-penetrating peptide TAT, and meanwhile, a bacillus subtilis secretory expression system is established to realize large-scale preparation of 62Nb and a recombinant thereof; finally, the replication process of the PDCoV is inhibited in an intracellular expression or exogenous delivery mode. Meanwhile, the invention provides a large-scale preparation method and application of the nano antibody, and provides a new technical scheme for antiviral prevention and detection of PDCoV.

The application relates to the technical field of monoclonal antibodies, and specifically discloses an anti-p-Tau181 antibody and a preparation method and application thereof. The preparation method of the anti-p-Tau181 antibody comprises the following steps: taking a p-Tau181 polypeptide IPAKTPPAPK(pT)PPSSGEPPK as an immunogen, injecting the immunogen into experimental animals to perform an immune response, and obtaining spleen lymphocytes of the experimental animals; taking myeloma cells and the spleen lymphocytes to perform mixed culture to obtain fusion cells; culturing the fusion cells, obtaining supernatant, and performing ELISA detection; the polypeptide used for detection is the p-Tau181 polypeptide; taking the hybridoma cell strain with a positive detection result to perform counter screening with a p-Tau217 polypeptide, a p-Tau231 polypeptide and t-tau protein; screening a positive clone which has a reaction to the p-Tau181 polypeptide and has no reaction to the p-Tau217 polypeptide, the p-Tau231 polypeptide and the t-tau protein; performing multiple cloning screening; obtaining a hybridoma cell strain which can secrete antibodies every time; and taking the antibodies secreted by the hybridoma cell strain as the anti-p-Tau181 antibody. The anti-p-Tau181 antibody can be specifically combined with the p-Tau181, and thus can be used for detecting Alzheimer's disease.

The invention discloses a bionic photo-thermal nano motor for targeted regulation and control of a solid tumor matrix-immunosuppression microenvironment and a preparation method and application thereof, and belongs to the technical field of biomedical materials.The preparation method comprises the steps that polydopamine nanoparticles and a K2PtCl4 aqueous solution are subjected to condensation reflux, and asymmetric PDA-Pt is prepared; and preparing a cancer-related fibroblast and breast cancer cell fusion membrane and coating the surface of PDA-Pt with the cancer-related fibroblast and breast cancer cell fusion membrane. The nano-motor realizes dual targeting of matrix cells and tumor cells through a surface modified fusion cell membrane, targeted ablation CAFs regulates and controls a tumor matrix microenvironment, a good tumor permeation effect is realized by cooperating with H2O2 responsive self-driving of the nano-motor, a mild photo-thermal-catalytic synergistic effect is formed by a photo-thermal effect of PDA and peroxidase-like activity of Pt nano-enzyme, and a good tumor permeation effect is achieved. And finally, the tumor immunosuppression microenvironment is remarkably improved, and the tumor immunotherapy effect is improved.

The invention discloses a preparation method and application of an IP10-fusion cell vesicle microneedle vaccine with a T-cell activation function, IP10-fusion cell vesicles with the T-cell activation function are prepared into the microneedle vaccine, on the immune activation level, the IP10-fusion cell vesicles have a vesicle antigen presentation function through the chemotactic effect of IP10, and the T-cell activation function of the IP10-fusion cell vesicles is achieved. T cell-mediated anti-tumor immune response can be efficiently excited, and a new path is provided for solving the problem of tumor immune escape; in the aspect of a delivery system, the minimally invasive slow release characteristic of the microneedle not only reduces the acceptance resistance of a patient, but also can maintain lasting immunostimulation, and compared with a traditional injection mode, the compliance and the treatment effect of the vaccine are remarkably improved. In addition, good biocompatibility ensures the safety of clinical application, and has great transformation potential in the field of tumor prevention and treatment.

The invention discloses a sheep oestrus monitoring system based on cell image morphology recognition, and the system is characterized in that the system comprises a sample collection module (100) which obtains a sheep vagina epithelial cell sample and carries out the dyeing of the cell sample; the microscopic imaging module (200) performs optical amplification on the dyed cell sample to generate a cell morphology image; the image processing module (300) extracts cell edge contours and quantifies morphological irregular features; and the intelligent analysis module (400) judges the estrus state of the sheep by comprehensively analyzing the nuclear off-center proportion and the form irregularity index. The sheep oestrus monitoring system based on cell image morphological recognition has the advantages that high-sensitivity capture of biological markers in the early oestrus stage is achieved by fusing dual morphological criteria of cell nucleus spatial displacement analysis and contour geometric distortion detection, and the timeliness and accuracy of reproduction regulation and control are remarkably improved.

The application provides a single-cell RNA sequencing data clustering method and system based on a multi-scale hypergraph, a multi-scale similarity matrix is constructed from a gene expression matrix; a cell hypergraph is generated from the multi-scale similarity matrix; cell hypergraph fusion is performed; SVD decomposition is performed on the hypergraph Laplacian matrix of the fused cell hypergraph; the feature vector corresponding to the first minimum eigenvalue is extracted, K-means is performed to obtain a clustering result, and the function of improving the clustering effect of single-cell RNA sequencing data is realized. The application proposes a novel hypergraph generation method, better captures the high-order correlation between multiple cells, and more accurately captures the correlation between cells using multiple scales. Compared with the existing single-cell RNA sequencing data clustering method, the application proposes a unified clustering framework, which comprehensively learns the topological information between cells on multiple scales, constructs a cell hypergraph for clustering, and can obtain a better clustering result.

The application provides a tumor immune microenvironment multi-cell recognition and spatial analysis system, comprising: an intrinsic signal generation module generating an initial intrinsic signal vector; a preliminary functional marker module generating a preliminary phenotype classification label map based on spatial centroid coordinates of cell objects; a functional neighborhood construction module calculating and generating a functional neighborhood feature vector; a composite feature splicing module constructing a composite state feature vector; an effective function determination module decoding to obtain a continuous value feature vector output by a function determination model representing the final function strength under the regulation of the microenvironment; and a functional map rendering module rendering to generate a cell effective function map under the regulation of the environment. The application solves the problem that, in the prior art, when interpreting cell functions, the intrinsic state information of the cells and the local functional microenvironment context information cannot be effectively fused, resulting in deviation in the description of the spatial distribution map of tumor microenvironment function heterogeneity.

The invention discloses an anti-p53R175H / HLA-A 02: 01 monoclonal antibody, a hybridoma cell strain S2-C33 for preparing the monoclonal antibody as well as a preparation method and application of the monoclonal antibody. The preparation method of the hybridoma cell strain comprises the following steps: immunizing a BALB / c mouse by taking human p53R175H / HLA-A 02: 01 recombinant protein as an immunogen to prepare an immunized mouse; taking splenocytes of the immunized mouse, and fusing the splenocytes with the SP2 / 0 myeloma cells to prepare fusion cells; screening monoclonal antibody positive fusion cells capable of secreting a compound specifically combined with p53R175H / HLA-A 02: 01 from the fusion cells, wherein the monoclonal antibody positive fusion cells are the hybridoma cell strain. The monoclonal antibody secreted by the hybridoma cell strain can be specifically combined with p53R175H / HLA-A 02: 01 presented on the surface of cancer cells, and the specificity is relatively high.

This invention discloses a hybridoma cell line containing the herbicide-resistant protein GAT, its produced antibody, and its applications. The hybridoma cell line is deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 46332. The preparation method includes: a) purifying prokaryotic expression to obtain recombinant GAT protein; b) immunizing animals: using the recombinant GAT protein as an antigen to immunize BALB / c mice; c) cell fusion: collecting spleen cells from immunized BALB / c mice and fusing them with SP2 / 0 cells; d) cell line establishment: subcloning using the limiting dilution method, performing ELISA detection on subclones after 5-7 days, until a stable hybridoma cell line secreting positive antibodies is selected for expansion, reculturing, and preservation. The monoclonal antibody secreted by the hybridoma cell line of this invention lays the foundation for the detection of the herbicide-resistant protein GAT in transgenic crops, thus supporting the development of transgenic crops. street The development, seed production, and preservation of genetically modified crops have ensured the survival of genetically modified organisms (GMOs). street The industrialization of genetically modified crops is progressing steadily, and the sustainable application of related transformants is advancing.

The application discloses a preparation method of a biomimetic membrane coated nano preparation based on regulation of in-vivo distribution of nanomedicine by fusing cell membranes and application thereof, and belongs to the field of biological medicine and nanometer delivery technology. The method fuses 4T1 cell membranes and red blood cell membranes at a certain ratio, and coats the fused membranes on the surface of nanoparticles to construct the biomimetic membrane coated nano preparation. The method can regulate the in-vivo distribution behavior of nanomedicine, so that the nanomedicine can exhibit spleen-biased distribution while maintaining certain tumor recognition capability. The preparation has the advantages of simple preparation method, good biocompatibility and strong regulation, and can be used for preparing a drug for preventing and / or treating breast cancer.

A method of preparing a genome-edited cell, in which a nucleic acid contained in a donor nucleic acid is introduced into the genome of a fused cell during the meiosis process thereof, and a method of promoting hybridization in which two or more types of cells are caused to hybridize during the meiosis process of the fused two or more types of cells having different traits.

The invention discloses a preparation method and application of a fusion cell membrane based on a DNA marker, and belongs to the technical field of biological medicine. The method comprises the following steps: constructing a DNA marking module 1 containing a coding sequence of a target membrane protein, incubating the DNA marking module 1 and an engineered and modified living cell together, and specifically coupling the DNA marking module to the membrane protein on the surface of a cell membrane through a click chemical reaction to obtain a living cell containing a DNA marker; pEG (Polyethylene Glycol) 6000 is adopted to induce N living cells containing DNA (Deoxyribose Nucleic Acid) markers to generate cell membrane fusion, and a fusion cell membrane system with multiple membrane protein information is constructed. The membrane components of a plurality of different DNA labeled cell lines are fused on the same platform, and the method has the advantages of simplicity and convenience in operation, no need of protein purification, high targeting property and the like. The fusion cell membrane obtained by the method is suitable for identification, function analysis and mechanism research of the membrane protein in a natural state, and especially can realize efficient identification of a membrane protein target in a complex disease model.

This disclosure provides a system for improving productivity and quality of adeno-associated virus (AAV) vectors and virus-like particles produced from packaging cell lines. Different groups of genetic elements required to produce AAV vectors (encoding regions for Rep proteins, Cap proteins, and helper function) are separated into different modules, which are each placed under control of different inducible regulatory elements. Optionally, the cell line is progeny of fused cells, and is thereby optimized for higher levels of virus production. To manufacture the intended AAV vector or particle, packaging cells containing the suite of inducible modules are contacted with (1) a payload vector containing a marker protein or therapeutic cargo, and (2) the inducer molecules for each of the regulatory elements. The viral titer, ratio of full capsids, and functional titer can be optimized by tuning the amount and ratio of each of the inducer molecules used.

The invention discloses a CBA method reagent for detecting a GFAP antibody through fusion expression of GFAP alpha and GFAP epsilon and a detection method, and belongs to the technical field of neural immunodiagnosis. The fusion protein sequentially comprises the following functional domains: secretion signal peptide, a GFAP alpha partial sequence, a GFAP epsilon partial sequence, a transmembrane sequence and a fluorescent label. The core of the invention lies in that a fusion cell is constructed by using partial sequences of GFAP alpha and GFAP epsilon, and co-expression is realized in a host cell through a co-transfection technology, so that the sensitivity and specificity of detecting a CFAP antibody in a CBA method (cellular immunofluorescence method) can be remarkably improved.

The invention relates to a breast cancer prognosis related biomarker, a detection kit and a biochip, the breast cancer prognosis related biomarker is a fusion cell formed by fusing an epithelial tumor cell and a myeloid cell, and the fusion cell comprises an epithelial cell molecular marker and a myeloid cell molecular marker; wherein the epithelial cell molecular marker comprises at least one of EPCAM and PanCK; the myeloid cell molecular marker comprises at least one of CD14 and CD68; the biomarker is used for breast cancer metastasis risk assessment. The liquid biopsy marker has the beneficial effects that the liquid biopsy marker for the metastasis risk of the breast cancer patient is provided and can be used as an independent prognosis index of the breast cancer metastasis risk.

The invention belongs to the field of fusion proteins, and particularly relates to a super positive charge polypeptide and NR4A1 truncated fragment fusion protein and application thereof. The amino acid sequence of the fusion protein IDR-SCP of the super positive charge polypeptide and the truncated fragment of the orphan nuclear receptor NR4A1 is as shown in SEQ ID NO. 2. On the basis of retaining the functional activity of the NR4A1 truncated fragment, the cell membrane penetration efficiency of the protein is effectively improved by fusing the cell penetrating peptide SCP, and the intracellular delivery effect of the functional fragment is remarkably improved. The IDR-SCP provided by the invention has the treatment effects of improving NASH mouse liver injury, inhibiting inflammation and degrading liver fibrosis.

The application discloses a kind of based on cell image morphological identification's sheep estrus monitoring system, including its features in, including sample acquisition module (100) obtains sheep vaginal epithelial cell sample and carries out dyeing treatment to cell sample;Microscopic imaging module (200) carries out optical amplification to the cell sample after dyeing and generates cell morphology image;Image processing module (300) extracts cell edge contour and quantifies morphological irregular feature;Intelligent analysis module (400) is determined by the comprehensive analysis to nuclear deviation center proportion and morphological irregular index, judges sheep estrus state.The application has beneficial effect for proposing a kind of based on cell image morphological identification's sheep estrus monitoring system, by fusing the double morphological criterion of nuclear space displacement analysis and contour geometric distortion detection, realizes the high sensitivity capture to early estrus biological marker, significantly improves the timeliness and precision of reproduction control.

The application discloses a CBA method reagent for detecting GFAP antibody by fusing expression of GFAP alpha and GFAP epsilon and a detection method, and belongs to the technical field of neuroimmunological diagnosis. The reagent comprises a host cell which is recombined to express a fusion protein. The fusion protein comprises the following functional domains in sequence: a secretion signal peptide, a GFAP alpha partial sequence, a GFAP epsilon partial sequence, a transmembrane sequence and a fluorescent label. The core of the application is that the fusion cell is constructed by using the partial sequences of GFAP alpha and GFAP epsilon, and the co-expression is realized in the host cell by using the co-transfection technology. The sensitivity and the specificity of the detection of the CFAP antibody in the CBA method (cellular immunofluorescence method) can be significantly improved.