11 results about "Syncytial cell" patented technology

The invention discloses an osteoclast culture method as well as a culture medium and application thereof, the culture method is based on primary monocyte extraction, inoculation is performed according to a specific density, then quantitative M-CSF is added, and culture is performed overnight; on the second day, when the cell confluence degree of the mononuclear bone marrow hematopoietic stem cells is 5-30%, supplementing quantitative RANKL into each hole, continuously culturing for 2-4 days, then changing the solution, and obtaining mature osteoclasts on the sixth-seventh day. According to the invention, the optimization relationship among the cell activity, the cell density and the culture medium in the primary culture process of the osteoclast is deeply studied, and from the perspective of cost reduction and efficiency improvement, the use cost of cell factors is remarkably reduced, the number of times of liquid change is reduced, and the traditional culture time of 10-12 days (calculated from cell extraction) is shortened to 6-7 days. The average diameter of the mature osteoclast cultured by the method is larger, the number of fused cell nucleuses is larger, the space proportion of the mature osteoclast is not less than 80%, and the cell quality is obviously higher than that in the prior art.

The application provides a tumor immune microenvironment multi-cell recognition and spatial analysis system, comprising: an intrinsic signal generation module generating an initial intrinsic signal vector; a preliminary functional marker module generating a preliminary phenotype classification label map based on spatial centroid coordinates of cell objects; a functional neighborhood construction module calculating and generating a functional neighborhood feature vector; a composite feature splicing module constructing a composite state feature vector; an effective function determination module decoding to obtain a continuous value feature vector output by a function determination model representing the final function strength under the regulation of the microenvironment; and a functional map rendering module rendering to generate a cell effective function map under the regulation of the environment. The application solves the problem that, in the prior art, when interpreting cell functions, the intrinsic state information of the cells and the local functional microenvironment context information cannot be effectively fused, resulting in deviation in the description of the spatial distribution map of tumor microenvironment function heterogeneity.

The invention belongs to the technical field of biological medicine, and discloses a nano antibody for resisting porcine delta coronavirus nucleocapsid protein as well as a recombinant, preparation and application of the nano antibody. According to the nano antibody for resisting the porcine delta coronavirus nucleocapsid protein and the recombinant nano antibody thereof, the specific nano antibody 62Nb targeting the PDCoV N protein is obtained through screening, efficient intracellular delivery of the nano antibody 62Nb is realized through engineering modification of a fusion cell-penetrating peptide TAT, and meanwhile, a bacillus subtilis secretory expression system is established to realize large-scale preparation of 62Nb and a recombinant thereof; finally, the replication process of the PDCoV is inhibited in an intracellular expression or exogenous delivery mode. Meanwhile, the invention provides a large-scale preparation method and application of the nano antibody, and provides a new technical scheme for antiviral prevention and detection of PDCoV.

The application provides a tumor immune microenvironment multi-cell recognition and spatial analysis system, comprising: an intrinsic signal generation module generating an initial intrinsic signal vector; a preliminary functional marker module generating a preliminary phenotype classification label map based on spatial centroid coordinates of cell objects; a functional neighborhood construction module calculating and generating a functional neighborhood feature vector; a composite feature splicing module constructing a composite state feature vector; an effective function determination module decoding to obtain a continuous value feature vector output by a function determination model representing the final function strength under the regulation of the microenvironment; and a functional map rendering module rendering to generate a cell effective function map under the regulation of the environment. The application solves the problem that, in the prior art, when interpreting cell functions, the intrinsic state information of the cells and the local functional microenvironment context information cannot be effectively fused, resulting in deviation in the description of the spatial distribution map of tumor microenvironment function heterogeneity.

The invention discloses an anti-p53R175H / HLA-A 02: 01 monoclonal antibody, a hybridoma cell strain S2-C33 for preparing the monoclonal antibody as well as a preparation method and application of the monoclonal antibody. The preparation method of the hybridoma cell strain comprises the following steps: immunizing a BALB / c mouse by taking human p53R175H / HLA-A 02: 01 recombinant protein as an immunogen to prepare an immunized mouse; taking splenocytes of the immunized mouse, and fusing the splenocytes with the SP2 / 0 myeloma cells to prepare fusion cells; screening monoclonal antibody positive fusion cells capable of secreting a compound specifically combined with p53R175H / HLA-A 02: 01 from the fusion cells, wherein the monoclonal antibody positive fusion cells are the hybridoma cell strain. The monoclonal antibody secreted by the hybridoma cell strain can be specifically combined with p53R175H / HLA-A 02: 01 presented on the surface of cancer cells, and the specificity is relatively high.

The application discloses a preparation method of a biomimetic membrane coated nano preparation based on regulation of in-vivo distribution of nanomedicine by fusing cell membranes and application thereof, and belongs to the field of biological medicine and nanometer delivery technology. The method fuses 4T1 cell membranes and red blood cell membranes at a certain ratio, and coats the fused membranes on the surface of nanoparticles to construct the biomimetic membrane coated nano preparation. The method can regulate the in-vivo distribution behavior of nanomedicine, so that the nanomedicine can exhibit spleen-biased distribution while maintaining certain tumor recognition capability. The preparation has the advantages of simple preparation method, good biocompatibility and strong regulation, and can be used for preparing a drug for preventing and / or treating breast cancer.

The invention discloses a preparation method and application of a fusion cell membrane based on a DNA marker, and belongs to the technical field of biological medicine. The method comprises the following steps: constructing a DNA marking module 1 containing a coding sequence of a target membrane protein, incubating the DNA marking module 1 and an engineered and modified living cell together, and specifically coupling the DNA marking module to the membrane protein on the surface of a cell membrane through a click chemical reaction to obtain a living cell containing a DNA marker; pEG (Polyethylene Glycol) 6000 is adopted to induce N living cells containing DNA (Deoxyribose Nucleic Acid) markers to generate cell membrane fusion, and a fusion cell membrane system with multiple membrane protein information is constructed. The membrane components of a plurality of different DNA labeled cell lines are fused on the same platform, and the method has the advantages of simplicity and convenience in operation, no need of protein purification, high targeting property and the like. The fusion cell membrane obtained by the method is suitable for identification, function analysis and mechanism research of the membrane protein in a natural state, and especially can realize efficient identification of a membrane protein target in a complex disease model.

This disclosure provides a system for improving productivity and quality of adeno-associated virus (AAV) vectors and virus-like particles produced from packaging cell lines. Different groups of genetic elements required to produce AAV vectors (encoding regions for Rep proteins, Cap proteins, and helper function) are separated into different modules, which are each placed under control of different inducible regulatory elements. Optionally, the cell line is progeny of fused cells, and is thereby optimized for higher levels of virus production. To manufacture the intended AAV vector or particle, packaging cells containing the suite of inducible modules are contacted with (1) a payload vector containing a marker protein or therapeutic cargo, and (2) the inducer molecules for each of the regulatory elements. The viral titer, ratio of full capsids, and functional titer can be optimized by tuning the amount and ratio of each of the inducer molecules used.

The invention belongs to the field of fusion proteins, and particularly relates to a super positive charge polypeptide and NR4A1 truncated fragment fusion protein and application thereof. The amino acid sequence of the fusion protein IDR-SCP of the super positive charge polypeptide and the truncated fragment of the orphan nuclear receptor NR4A1 is as shown in SEQ ID NO. 2. On the basis of retaining the functional activity of the NR4A1 truncated fragment, the cell membrane penetration efficiency of the protein is effectively improved by fusing the cell penetrating peptide SCP, and the intracellular delivery effect of the functional fragment is remarkably improved. The IDR-SCP provided by the invention has the treatment effects of improving NASH mouse liver injury, inhibiting inflammation and degrading liver fibrosis.

The application discloses a kind of based on cell image morphological identification's sheep estrus monitoring system, including its features in, including sample acquisition module (100) obtains sheep vaginal epithelial cell sample and carries out dyeing treatment to cell sample;Microscopic imaging module (200) carries out optical amplification to the cell sample after dyeing and generates cell morphology image;Image processing module (300) extracts cell edge contour and quantifies morphological irregular feature;Intelligent analysis module (400) is determined by the comprehensive analysis to nuclear deviation center proportion and morphological irregular index, judges sheep estrus state.The application has beneficial effect for proposing a kind of based on cell image morphological identification's sheep estrus monitoring system, by fusing the double morphological criterion of nuclear space displacement analysis and contour geometric distortion detection, realizes the high sensitivity capture to early estrus biological marker, significantly improves the timeliness and precision of reproduction control.

The application discloses a CBA method reagent for detecting GFAP antibody by fusing expression of GFAP alpha and GFAP epsilon and a detection method, and belongs to the technical field of neuroimmunological diagnosis. The reagent comprises a host cell which is recombined to express a fusion protein. The fusion protein comprises the following functional domains in sequence: a secretion signal peptide, a GFAP alpha partial sequence, a GFAP epsilon partial sequence, a transmembrane sequence and a fluorescent label. The core of the application is that the fusion cell is constructed by using the partial sequences of GFAP alpha and GFAP epsilon, and the co-expression is realized in the host cell by using the co-transfection technology. The sensitivity and the specificity of the detection of the CFAP antibody in the CBA method (cellular immunofluorescence method) can be significantly improved.