Uricase/catalase lipid nanoparticles wrapped by fusion cell membrane and preparation method of uricase/catalase lipid nanoparticles

A technology of catalase lipid and catalase, which is applied in the direction of nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of low targeting, low bioavailability, poor stability, etc., and achieve good stability , good immunogenicity and enhanced stability

Active Publication Date: 2022-04-12
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fused cell membrane-wrapped uricase / catalase lipid nanoparticles overcome the shortcomings of uricase, such as poor stability, easy to be degraded by enzymes, low bioavailability, and low targeting, and can maintain a long-term blood drug concentration. release effect, can reduce the number of administrations, improve patient compliance, and reduce toxic and side effects

Method used

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  • Uricase/catalase lipid nanoparticles wrapped by fusion cell membrane and preparation method of uricase/catalase lipid nanoparticles
  • Uricase/catalase lipid nanoparticles wrapped by fusion cell membrane and preparation method of uricase/catalase lipid nanoparticles
  • Uricase/catalase lipid nanoparticles wrapped by fusion cell membrane and preparation method of uricase/catalase lipid nanoparticles

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Embodiment 1

[0028] Fused cell membrane-wrapped uricase / catalase lipid nanoparticles include uricase / catalase lipid nanoparticles and erythrocyte / macrophage fusion cell membrane envelope. The weight ratio of each component in the uricase / catalase lipid nanoparticle formula is: 2 parts of lecithin, 1 part of cholesterol, 200 parts of boric acid-borax buffer solution, the buffer solution concentration is 50mM, and the buffer solution pH is 7, The uricase content was 0.1 mg / mL, and the catalase content was 0.05 mg / mL.

[0029] Preparation method: (1) Preparation method of uricase / catalase lipid nanoparticles: dissolve uricase and catalase in a buffer solution with a prescription amount in a mass ratio (2:1) to form uricase / catalase Catalase solution, weigh the prescribed amount of lecithin and cholesterol and dissolve in 10mL of dichloromethane organic solvent, remove the organic solvent by rotary evaporation to form a uniform lipid film, add uricase / catalase solution, shake in a water bath a...

Embodiment 2

[0031] Fused cell membrane-wrapped uricase / catalase lipid nanoparticles include uricase / catalase lipid nanoparticles and erythrocyte / macrophage fusion cell membrane envelope. The weight ratio of each component in the uricase / catalase lipid nanoparticle formula is: 2.7 parts of lecithin, 2.1 parts of cholesterol, 418.2 parts of N, N-bishydroxyethylglycine-sodium hydroxide buffer solution, buffer solution The concentration is 120mM, the pH of the buffer solution is 7.3, the content of uricase is 0.07mg / mL, and the content of catalase is 0.14mg / mL.

[0032] Preparation method: (1) Preparation method of uricase / catalase lipid nanoparticles: dissolve uricase and catalase in a buffer solution with a prescription amount in a mass ratio (1:2) to form uricase / catalase Catalase solution, weigh the prescribed amount of lecithin and cholesterol and dissolve in 15mL chloroform organic solvent, remove the organic solvent by rotary evaporation to form a uniform lipid film, add uricase / catala...

Embodiment 3

[0034] Fused cell membrane-wrapped uricase / catalase lipid nanoparticles include uricase / catalase lipid nanoparticles and erythrocyte / macrophage fusion cell membrane envelope. The weight ratio of each component in the uricase / catalase lipid nanoparticle formula is: 3.4 parts of lecithin, 2.6 parts of cholesterol, 527.3 parts of sodium carbonate-sodium bicarbonate buffer solution, buffer solution concentration is 130mM, buffer solution pH was 7.6, the uricase content was 0.1 mg / mL, and the catalase content was 0.15 mg / mL.

[0035] Preparation method: (1) Preparation method of uricase / catalase lipid nanoparticles: dissolve uricase and catalase in the buffer solution of prescription amount with mass ratio (1:1.5) to form uricase / catalase Catalase solution, weigh the prescribed amount of lecithin and cholesterol and dissolve in 20mL of anhydrous ethanol organic solvent, remove the organic solvent by rotary evaporation to form a uniform lipid film, add uricase / catalase solution, sha...

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Abstract

The invention belongs to the field of pharmaceutical preparations. The invention relates to a uricase / catalase lipid nanoparticle wrapped by a fusion cell membrane and a preparation method of the uricase / catalase lipid nanoparticle. According to the prepared fusion cell membrane coated uricase / catalase lipid nanoparticles, the stability of uricase can be improved, the protease hydrolysis resistance of uricase is improved, the bioavailability is improved, the inflammation chemotactic ability is enhanced, and the toxic and side effects are reduced.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to fusion cell membrane-wrapped uricase / catalase lipid nanoparticles and a preparation method thereof. Background technique [0002] Hyperuricemia is caused by an imbalance between the rate of production and excretion of uric acid. Long-term hyperuricemia can lead to gout, and persistent hyperuricemia is closely related to the induction of cardiovascular disease, kidney disease, arthritis, insulin resistance and diabetes. At present, the main method of clinical treatment of hyperuricemia is to prevent uric acid synthesis and promote uric acid excretion by inhibiting xanthine oxidase. The chemical drugs for the treatment of hyperuricemia have obvious liver and kidney toxicity, which will cause serious adverse reactions to the body. Uricase is a highly efficient oxidase that can oxidize uric acid in the body to isopropane and hydrogen peroxide, and can quickly and effectivel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K9/52A61K47/46A61P19/06B82Y5/00B82Y40/00
CPCY02A50/30
Inventor 张景勍袁誉铭曹鑫钟彩灵王婷婷陈冉
Owner CHONGQING MEDICAL UNIVERSITY
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