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A kind of extracellular ratio type oxygen sensing probe and its preparation method and application

An oxygen sensing probe, ratiometric technology, applied in the field of extracellular ratiometric oxygen sensing probe and its preparation, can solve problems such as low quantum efficiency

Active Publication Date: 2018-11-27
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some hydrophilic oxygen probes prepared from metalloporphyrins (PtCPK, PtCP, PdCPK, or PtTCPP) exhibit extremely low quantum efficiencies ranging from 0.001 to 0.0095

Method used

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  • A kind of extracellular ratio type oxygen sensing probe and its preparation method and application
  • A kind of extracellular ratio type oxygen sensing probe and its preparation method and application
  • A kind of extracellular ratio type oxygen sensing probe and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] In this example, the polymer carrier shown in formula a was synthesized through the following process:

[0131]

[0132] where A is

[0133] The synthesis steps are as follows:

[0134] P1 synthesis process:

[0135] 988mg (8.7mmol) ε-caprolactone and 61.1mg (0.1mmol) aggregation-induced luminescent molecule A-OH (synthesized according to the following literature: Hongguang Lu, et al, JOURNAL OF POLYMER SCIENCE PART A: POLYMERCHEMISTRY 2012,50,890– 899) was fully mixed in the flask, and stirred in an oil bath at 110°C under a nitrogen atmosphere for 5 minutes, and then injected two drops of catalyst stannous octoate (Sn(Oct) 2 ), after stirring and reacting overnight, the reactant was rapidly cooled in an ice bath to obtain a solid polymer. Afterwards, the solid polymer was redissolved in 5 mL of dichloromethane, and then the solution was slowly added dropwise to 100 mL of ice methanol to obtain a polymer precipitation, and the precipitation process was repeated...

Embodiment 2

[0141] In this example, the polymer carrier in formula d is prepared by the following reaction

[0142]

[0143] 73mg (0.06mmol) P2, 440mg (0.8mmol) oligoethylene glycol methyl ether methacrylate (OEGMA, molecular weight 550), 10mg AIBN were placed in a 10mL Schlenk tube, and filled with nitrogen after using the traditional deoxygenation method The gas was exchanged three times, heated to 70°C and stirred, and reacted for 24 hours. After the reaction was completed, it was added dropwise into glacial ether to obtain viscous polymer P4. Yield 84%. Mn=35400, Mw=43200. x:y=7.5:72.5.

Embodiment 3

[0145] The ability of the polymer carrier prepared in Example 1 to form micelles in an aqueous solution is investigated by measuring its critical micelle concentration. During the formation of micelles, the fluorescence emission will be quenched by water to obtain a weaker micelle concentration. fluorescence. Once micelles are formed, the fluorescence intensity will be greatly enhanced. The CMC of the polymer carrier prepared in Example 1 was judged by measuring the change of the fluorescence intensity of P3 at a concentration of 0.01 μg / mL to 100 μg / mL in an aqueous solution, and judging the sudden change point of the fluorescence intensity. During the test process, the fluorescence intensity changes with the concentration as figure 1 As shown, the visible nanosystem has a strong absorption at a wavelength of 530 nanometers, and the fluorescence intensity increases with the increase of the polymer concentration. The concentration and the fluorescence intensity value at 530n...

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Abstract

The invention provides a ratio-type oxygen sensing probe and its preparation method and application. The ratio-type oxygen sensing probe includes a carrier and an oxygen-sensitive compound wrapped in the carrier. The carrier is grafted and polymerized by amphiphilic substance and the aggregation-inducing luminescence group connected on the amphiphilic graft polymer. The ratiometric oxygen sensing probe of the present invention can measure the oxygen consumption of Escherichia coli and GM12878 human B lymphocytes at different cell concentrations, and at the same time it can measure at least 100cfu / mL bacteria in situ within 7 hours, and can also be used for Drug screening, and its non-cytotoxicity, safe and reliable. In addition, because the PtTFPP probe micelles exhibit extremely high quantum efficiency, and this oxygen sensing probe can measure the oxygen content in aqueous solution, it can be widely used in some commercial instruments similar to microplate readers, which can provide Aids in high-throughput measurements, disease diagnosis, and metabolism research.

Description

technical field [0001] The invention belongs to the field of nano medical materials, and relates to a ratio-type oxygen sensing probe and its preparation method and application, in particular to an extracellular ratio-type oxygen sensing probe and its preparation method and application. Background technique [0002] Oxygen is a very important element for the environment, ocean, agriculture, industry, health, etc. In the biological field, oxygen, as a key basic substance in aerobic cell nuclear organization, plays an extremely important role in many core cell physiology and developmental processes including energy production, energy balance, and biomass generation. As an electron acceptor, oxygen is critical for electron transfer (ETC) functions in mitochondria and ATP production. Oxygen availability and / or availability, over time, contributes to pathology and disease states and thus represents an important goal of medical diagnostics and basic research. For example, it has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C09K11/02C08F290/06C08F220/58C08F220/28G01N21/64
CPCG01N21/6402G01N21/6486C09K11/02C09K11/06C08F290/061C09K2211/185C08F220/286C08F220/58
Inventor 田颜清邹贤劭潘婷婷蒋嘉培
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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