Oyster bioactive peptide extraction method

Abstract

The invention belongs to the technical field of biology, and relates to a method for performing enzymolysis of oyster protein by an enzymolysis method and separating and purifying oyster bioactive peptide by column chromatography. The method includes the steps: shelling, cleaning and homogenizing oysters, adding distilled water to obtain oyster serous fluid, and extracting the oyster serous fluid at low temperature; flocculating the oyster serous fluid, centrifugally removing residues, taking supernate, freeze-drying the supernate, separating the supernate by a Sephacryl s-100 column or performing enzymolysis and enzyme deactivation of the oyster serous fluid, centrifugally removing the residues and freeze-drying the oyster serous fluid to obtain crude extracts and performing gel column chromatography for the crude extracts; collecting peaks, separating and purifying the peaks by ion exchange column chromatography, collecting active peaks, freeze-drying the peaks and then desalting the peaks by a gel column to obtain pure oyster natural bioactive polypeptides. The oyster natural bioactive polypeptides obtained by the preparation method are high in purity, have antitumor activity, are naturally extracted substances and can be developed and used as a medicine, and biological activity of the oyster natural bioactive polypeptides is effectively protected in the extraction process.

Description

[0001] Technical field: [0002] The invention belongs to the field of biotechnology, and relates to a preparation method for separating active peptides by using oysters as main raw materials through enzymolysis and other extraction techniques, in particular to an extraction method for oyster active peptides. [0003] Background technique: [0004] Oyster is a kind of marine shellfish. In classification, oyster belongs to Molluscs, Phallobranches, Heterocylinda, Oyster Superfamily, Oysteridae. It is a kind of bivalve seafood that is used for both food and medicine. There are farming in coastal areas, especially in Guangdong Province, which has developed rapidly in recent years. Oyster meat is milky white, tender and nutritious. In addition to rich protein, vitamins and carbohydrates and other nutrients, it also contains more than ten kinds of amino acids necessary for the human body. Mineral nutrients, etc., modern research has also proved that oysters are rich in glycogen, whi...

AI Technical Summary

Problems solved by technology

Oyster extract has antibacterial effect, has inhibitory effect on poliovirus and influenza virus, and its water-soluble components can still improve the immunity of animal organisms, etc. Oysters are rich in biologically active substances, which have significant health care effects; from The research on extracting active substances from oysters has been reported for a long time, and oyster health food with significant medical and health effects has been sold in large quantities. For example, Shenzhen Neptune Group has achieved remarkable economic and social benefits in the development and research of oyster-related products. In order to further promote the industrialization of this project, efforts are being made to develop efficient oyster health food and medicines. The patent document "A Preparation Method for Oyster Active Peptides" uses Pacific oysters as raw materials, and adopts enzymatic hydrolysis-nanofiltration desalination and concentration The method for preparing oyster active peptides belongs to the field of food biotechnology and does not involve the field of medicine. In recent years, researches on bioactive substances in oysters have been carried out in developed countries such as Europe, Japan, and the United States. Substances with significant biological activity, which have been developed as pharmaceuticals have not been reported