Rhodamine B double-channel fluorescence probe and application
A fluorescent probe and dual-channel technology, applied in fluorescence/phosphorescence, material analysis through optical means, luminescent materials, etc., to achieve the effects of saving detection costs, high detection sensitivity, and excellent selectivity
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Embodiment 1
[0025] Example 1: Synthesis of Rhodamine B Class Dual-Channel Fluorescent Probes
[0026] The reaction formula for synthesizing the rhodamine B class dual-channel fluorescent probe is as follows:
[0027]
[0028] Concrete steps for synthesizing Rhodamine B dual-channel fluorescent probes:
[0029] 1) Synthesis of Rhodamine B hydrazide intermediate M1: Weigh 1.2g Rhodamine B, dissolve it in 30mL absolute ethanol, stir vigorously at room temperature, add 3.0-5.0mL 85wt% hydrazine hydrate dropwise, after the dropwise addition is completed Continue to stir for 0.5h, then raise the temperature and reflux for 8-12h until the solution turns orange yellow, cool down, remove the solvent by rotary evaporation under reduced pressure, add 1N HCl50-70mL, add 1mol / L NaOH 70-80mL under stirring conditions until the solution pH is Around 9-10, a large amount of precipitation appeared, filtered with suction, washed with distilled water for 3 times, and dried overnight in a vacuum oven, th...
Embodiment 2
[0035] Embodiment 2: Rhodamine B class dual-channel fluorescent probe pair VO 2+ and Cu 2+ selective detection of
[0036] Add 200 μmol / L metal ion (Mg 2+ , Al 3+ , Ca 2+ , Cr 3+ , Mn 2+ , Fe 2+ , Fe 3+ ,Co 2+ , Ni 2+ , Zn 2+ , Ag + , Cd 2+ , Sn 2+ , Ba 2+ , Hg 2+ , Pb 2+ , VO 2+ , La 3+ , Ce 3+ , Pr 3+ ,Nd 3+, Sm 3+ , Eu 3+ , Dy 3+ , Er 3+ , Th 4+ , Cu 2+ ), after 0.5h, detect the changes in the fluorescence emission spectrum and the ultraviolet-visible absorption spectrum of the solution. It can be seen from the test results that the Rhodamine B dual-channel fluorescent probe itself has almost no emission peak at 586nm, and almost no absorption peak at 560nm; when adding VO 2+ After ionization, the Rhodamine B dual-channel fluorescent probe solution has a strong emission peak at 586nm. When Cu is added 2+ After ions, the rhodamine B dual-channel fluorescent probe solution showed a strong absorption peak at 560nm; however, other metal ions, such a...
Embodiment 3
[0037] Embodiment 3: Rhodamine B class dual-channel fluorescent probe pair VO 2+ and Cu 2+ Fluorescence titration experiment
[0038] 20 μmol / L Rhodamine B class dual-channel fluorescent probe dimethyl sulfoxide (DMSO) and 0.05mol / L, pH=8 Tris-HCl buffer volume ratio is a mixed solution of 7:3, gradually increase the addition VO 2+ and Cu 2+ After 0.5h, test the fluorescence spectrum and absorption spectrum of each sample. From the results, it can be seen that with VO 2+ and Cu 2+ With the gradual increase of the concentration, the fluorescence intensity of the Rhodamine B dual-channel fluorescent probe solution at 586nm increases gradually, and the absorption intensity at 560nm also increases gradually. When VO 2+ and Cu 2+ When the concentration of rhodamine B increases to about 400μmol / L, the titration reaches saturation, and the emission intensity and absorption intensity no longer increase; 2+ and Cu 2+ have good sensing properties.
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