A kind of rhodamine derivative bifunctional fluorescent probe and its application
A fluorescent probe and dual-function technology, which is applied in rhodamine derivative-based dual-functional fluorescent probes and its application fields, to achieve good sensing properties, low detection limit, and cost-saving detection effects
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Embodiment 1
[0025] Example 1: Synthesis of bifunctional fluorescent probes based on rhodamine derivatives
[0026] The reaction formula for synthesizing rhodamine derivative-based bifunctional fluorescent probes:
[0027]
[0028] Concrete steps for synthesizing rhodamine derivative-based bifunctional fluorescent probes:
[0029] 1) Synthesis of Rhodamine B hydrazide intermediate M1: Weigh 1.2g of Rhodamine B, dissolve it in 30mL of absolute ethanol, stir vigorously at room temperature, add 3.0-5.0mL of 85wt% hydrazine hydrate dropwise, and the addition is complete Then continue to stir for 0.5h, then raise the temperature and reflux for 12h until the solution turns orange yellow, cool, remove the solvent by rotary evaporation under reduced pressure, add 1N HCl 50-60mL, add 1mol / L NaOH 70-80mL under stirring conditions until the solution pH is Around 9-10, a large amount of precipitation appeared, filtered with suction, washed with distilled water for 3 times, and dried overnight in a...
Embodiment 2
[0035] Example 2: Rhodamine Derivatives Bifunctional Fluorescent Probes to VO 2+ and Cu 2+ Selective detection of:
[0036] 20 μmol / L rhodamine derivative bifunctional fluorescent probe dimethyl sulfoxide (DMSO) and 0.01mol / L, Tris-HCl buffer solution of pH=7.4 with a volume ratio of 4:1 mixed solution, add respectively 200μmol / L of metal ions (Mg 2+ , Al 3+ , Ca 2+ , Cr 3+ , Mn 2+ , Fe 2+ , Fe 3+ ,Co 2+ , Ni 2+ , Zn 2+ , Ag + , Cd 2+ , Sn 2+ , Ba 2+ , Hg 2+ , Pb 2+ , VO 2+, La 3+ , Ce 3+ , Pr 3+ ,Nd 3+ , Sm 3+ , Eu 3+ , Dy 3+ , Er 3+ , Th 4+ , Cu 2+ ), after 0.5h, detect the changes in the fluorescence emission spectrum and the ultraviolet-visible absorption spectrum of the solution. It can be seen from the detection results that the rhodamine derivative bifunctional fluorescent probe itself has almost no emission peak at 586nm, and almost no absorption peak at 560nm; when adding VO 2+ After ionization, the rhodamine derivative bifunctional fluore...
Embodiment 3
[0037] Example 3: Rhodamine Derivatives Bifunctional Fluorescent Probes to VO 2+ and Cu 2+ Fluorescence titration experiment:
[0038] 20 μmol / L rhodamine derivatives bifunctional fluorescent probe dimethyl sulfoxide (DMSO) and 0.05mol / L, pH=8 Tris-HCl buffer volume ratio is a mixed solution of 7:3, gradually increasing Joined VO 2+ and Cu 2+ After 0.5h, test the fluorescence spectrum and absorption spectrum of each sample. It can be seen from the test results that with VO 2+ and Cu 2+ As the concentration increases gradually, the fluorescence intensity of the rhodamine derivative bifunctional fluorescent probe solution at 586nm increases gradually, and the absorption intensity at 560nm also increases gradually. When VO 2+ and Cu 2+ When the concentrations of the rhodamine derivatives were increased to about 400 μmol / L, the titration reached saturation, and the emission intensity and absorption intensity no longer increased; 2+ and Cu 2+ have good sensing properties. ...
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