A method for continuously extracting high-purity lactic acid from lactic acid fermentation broth

A lactic acid fermentation, high-purity technology, applied in fermentation, separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of non-continuous production, large loss of lactic acid, cumbersome process, etc., to facilitate industrial scale-up and reduce Fermentation costs and the effect of improving production efficiency

Active Publication Date: 2020-08-04
NANJING TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of large loss of lactic acid, low yield, low purity, serious pollution, high energy consumption, cumbersome process and non-continuous production in the process of lactic acid separation and extraction, the present invention proposes a method for continuous extraction of high Methods of Purifying Lactic Acid

Method used

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  • A method for continuously extracting high-purity lactic acid from lactic acid fermentation broth
  • A method for continuously extracting high-purity lactic acid from lactic acid fermentation broth
  • A method for continuously extracting high-purity lactic acid from lactic acid fermentation broth

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Embodiment 1

[0046] like figure 1 As shown, a method for continuously extracting high-purity lactic acid from lactic acid fermentation broth comprises the following steps:

[0047] (1) Preparation of fermentation medium, specifically glucose 130g / L, yeast powder 15g / L, peptone 0.5g / L, magnesium sulfate 0.5g / L, sodium acetate 5g / L, prepared with deionized water; then Bacillus coagulans is the production strain, the inoculation amount is 10%; it is placed in a fermenter for anaerobic fermentation, the fermentation conditions are: temperature 50°C, ammonia water is used to adjust the pH of the fermentation broth to maintain at 6.5, the stirring speed is 150rpm, and lactic acid is obtained after 48 hours of fermentation fermentation broth. The liquid phase analysis diagram of each component of the obtained lactic acid fermentation broth is shown in the appendix figure 2 , containing many impurities in the lactic acid fermentation broth, the main components are glucose (retention time 4.7min...

Embodiment 2

[0054] A method for continuously extracting high-purity lactic acid from lactic acid fermentation broth, comprising the following steps:

[0055] (1) Preparation of fermentation medium, specifically glucose 110g / L, yeast powder 15g / L, peptone 0.5g / L, magnesium sulfate 0.5g / L, sodium acetate 5g / L, prepared with deionized water; then Bacillus coagulans is the production strain, the inoculation amount is 10%; it is placed in a fermenter for anaerobic fermentation, and the fermentation conditions are: temperature 60°C, ammonia water is used to adjust the pH of the fermentation broth to maintain at 6.5, the stirring speed is 200rpm, and lactic acid is obtained after 48 hours of fermentation fermentation broth;

[0056] (2) The 19-hole ceramic microfiltration membrane with a pore size of 0.2um is used for the preliminary separation step (1) Gained lactic acid fermentation liquid, the operating pressure is 0.3MPa, the volume of the permeated liquid of the microfiltration is 80% of th...

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Abstract

The invention discloses a method for continuously extracting high-purity lactic acid from a lactic acid fermented broth. The method comprises the following steps: (1) preparing a fermentation medium, inoculating lactic acid bacteria, and conducting anaerobic fermentation in a fermentation tank to obtain the lactic acid bacterium fermented broth; (2) using a micro-filtration membrane to primarily separate the lactic acid fermented broth obtained in the step (1), enabling a micro-filtration trapped fluid to flow back to the fermentation tank to continue to ferment, using a nano-filtration membrane to secondarily separate after using a micro-filtration permeate liquid to regulate the pH value, so as to obtain a nano-filtration trapped fluid and a nano-filtration permeate liquid; (3) taking the nano-filtration permeate liquid obtained in the step (2), refining in a multi-component simulated moving bed, concentrating and purifying a collected lactic acid solution to obtain a finished product of the lactic acid; enabling collected glucose and acetic acid to flow back to the fermentation tank for cyclic utilization. The method is green and environmentally friendly, the cyclic utilization of glucose and circular fermentation are realized, the inoculation amount is saved, the purity of the prepared finished product of the lactic acid is high, and the process continuity and the production efficiency are improved.

Description

technical field [0001] The invention belongs to lactic acid production technology, in particular to a method for continuously extracting high-purity lactic acid from lactic acid fermentation liquid. Background technique [0002] As one of the world's three major organic acids, lactic acid is widely used in the fields of food, cosmetics, leather, medicine, chemical industry and agriculture. With the increasing shortage of fossil resources such as petroleum, lactic acid as a renewable biomass resource is becoming more and more more and more people's attention. Lactic acid is also a raw material for the synthesis of polylactic acid (PLA). Compared with plastics synthesized from petrochemicals, PLA materials are biorenewable and biodegradable, and are considered to be a promising environment-friendly polymer material. . Not only the performance is equivalent to traditional plastics, rubber, etc., but also has excellent mechanical strength, toughness, and processing properties....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C07C51/42C07C51/47C07C59/08
CPCC07C51/42C07C51/47C12P7/56C07C59/08
Inventor 应汉杰宋明凯吴菁岚周精卫朱慧
Owner NANJING TECH UNIV
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