Test piece for quick test of coliform bacteria and preparation method and test method of test piece

A technology of coliforms and test strips, which is applied in the field of test strips for rapid detection of coliforms, can solve the problems of fuzzy colony edges and inaccurate interpretation of results, and achieve uniform distribution, avoid inaccurate counting results, and simple preparation methods Effect

Inactive Publication Date: 2017-06-09
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the target colony existing in the coliform test piece in the prior art diffuses and grows on the non-woven fabric, the edge of the colony is blu

Method used

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  • Test piece for quick test of coliform bacteria and preparation method and test method of test piece
  • Test piece for quick test of coliform bacteria and preparation method and test method of test piece
  • Test piece for quick test of coliform bacteria and preparation method and test method of test piece

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Rapidly detects the composition of the test piece of coliform group

[0032] Such as figure 1 As shown, a test piece for rapid detection of coliform bacteria includes a lower bottom plate 4, pearl paper 2, an upper film assembly 1, a test piece label 5 and a color-developing liquid culture medium for coliform bacteria. The lower bottom plate 4, the pearl paper 2 and the upper film assembly 1 are rectangular, and there is a circular groove 3 in the middle of the pearl paper 2, and the color-developing liquid culture medium characteristic of coliform bacteria is evenly distributed in the circular groove 3. Each 100mL coliform characteristic chromogenic liquid medium contains tryptone 1.0-3.0g, sodium chloride 0.1-1.0g, lactose 1.5-3.5g, bile salt 0.1-0.4g, yeast extract powder 0.2-1.0g, phosphoric acid Dipotassium hydrogen 0.15-0.5g, potassium dihydrogen phosphate 0.15-0.5g, cold water soluble gel 0.5-2g, bacteriostatic agent mixture 150-500IU / mL, chromogeni...

Embodiment 2

[0043] The preparation of the test piece of embodiment 2 rapid detection coliform group

[0044]2.1 Preparation of coliform-characteristic chromogenic liquid medium: 100 mL of coliform-characteristic chromogenic liquid medium was prepared by weighing 2.5 g of tryptone, 0.35 g of sodium chloride, 2.5 g of lactose, 0.2 g of bile salt, Yeast extract powder 0.6g, dipotassium hydrogen phosphate 0.35g, potassium dihydrogen phosphate 0.35g, cold water soluble gel 0.9g, add distilled water 100mL to the Erlenmeyer flask, stir to dissolve, and sterilize at 121℃ for 15min After cooling to room temperature, 35,000 IU of filter-sterilized bacteriostatic agent mixture and 0.03 g of chromogenic enzymatic hydrolysis substrate mixture were sequentially added to obtain a chromogenic liquid culture medium characteristic of coliform bacteria, which was refrigerated at 0-4°C for later use.

[0045] 2.2 Pretreatment of each component of the test piece:

[0046] 2.2.1 Pre-treatment of the upper fil...

Embodiment 3

[0050] The detection of coliform group in the sample to be tested of embodiment 3

[0051] When using the rapid detection coliform test piece of the present invention to detect coliform in a sample, the following steps are mainly included:

[0052] First, sample processing: take 25mL (g) of the sample and put it into a sampler or a homogenizing cup containing 225mL of sterile diluent (phosphate buffer or saline) to make a 1:10 homogeneous sample solution, and use 1mL of sterilized Take 1mL of 1:10 homogeneous sample solution with the bacterial pipette, inject it into a test tube containing 9mL sterile diluent, shake well to obtain a 1:100 sample diluent, follow the above steps to dilute successively, and prepare a 10-fold incremental series of samples Dilute homogenate. Generally, 2 to 3 dilutions are selected for testing, and liquid samples with a small amount of bacteria (such as drinking pure water and mineral water, etc.) can be directly absorbed for testing.

[0053] Se...

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Abstract

The invention provides a test piece for quick test of coliform bacteria. The test piece comprises a lower substrate, pearl paper, an upper thin film component and a coliform bacterial characteristic chromogenic fluid medium. The middle of the pearl paper is provided with a circular groove, and the coliform bacterial characteristic chromogenic fluid medium is uniformly distributed in the circular groove. The test piece for quick test of the coliform bacteria has advantages of easiness in distinguishing of target bacteria, chromogenic clearness, simplicity in result determination, reliability in test result, short test time, simplicity and convenience in operation, low cost and the like and can play a significant role in microbiological detection.

Description

technical field [0001] The invention belongs to the field of rapid detection, relates to microorganism detection technology, in particular to a test piece for rapid detection of coliform bacteria, a preparation method and a detection method of the test piece. Background technique [0002] Coliform bacteria are widely distributed and widely exist in feces of warm-blooded animals and in nature. Investigations and studies have shown that coliform bacteria mostly exist in the feces of warm-blooded animals, places where humans are frequently active, and places with feces pollution. The pollution of human and animal feces to the external environment is the main reason for the existence of coliforms in nature. Most of the feces are dominated by typical Escherichia coli, while other types of coliforms are more common in the external environment. Coliform bacteria is one of the important indicators to evaluate food hygiene quality, and it has been widely used in food hygiene work at...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/10C12Q1/04C12R1/19
CPCC12Q1/045C12Q1/10
Inventor 冯才伟何方洋万宇平湛剑龙侯璐伟周绘周小江贾玲玲付浩席芸芸王吉平易重任李文馨
Owner 贵州勤邦食品安全科学技术有限公司
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