Feed fermentation agent and application in feed fermentation

A starter and feed raw material technology, applied in the field of microbial application and feed fermentation, can solve the problems of no fermentation, etc., achieve the effect of improving feed nutritional value and digestion and absorption rate, simple operation process and low cost

Pending Publication Date: 2017-06-27
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yeast and lactic acid bacteria are often used as water quality regulators in aquaculture, and there are also research reports on feeding with mixing materials, but there are no research reports on feed or feed raw material fermentation

Method used

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  • Feed fermentation agent and application in feed fermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A feed starter, characterized in that the feed starter is mixed in equal proportions from the fermentation liquid of Candida utilis and Lactobacillus plantarum; the preservation number of Candida utilis: CGMCC2.281; No.: CGMCC1.511, the concentration of Candida utilis reached 3.0×10 8 -5.0×10 8 CFU / mL, the concentration of Lactobacillus plantarum reaches 1.0×10 9 -5.0×10 9 CFU / mL; its preparation steps are as follows:

[0023] (1) Preparation of Candida utilis fermentation broth

[0024] The yeast strains preserved on the slant were inoculated on a fresh YPD medium plate and cultured at 25°C for 48h. Pick a ring from the plate, inoculate it in a 250mL Erlenmeyer flask containing 100mL seed medium, and culture it with shaking at 25°C and 150r / min shaker for 24h.

[0025] (2) Preparation of Lactobacillus plantarum fermentation broth

[0026] The puncture cultured lactic acid bacteria strains were inoculated on a fresh lactic acid bacteria medium plate and cultured a...

Embodiment 2

[0031] The application of feed starter in the fermentation of feed raw materials, the operation steps are as follows:

[0032] (1) Feed ingredients have the following combinations: a) Soybean meal, glucose 1.0%, K 2 HPO 4 0.2%, b) peanut meal, glucose 1.0%, K 2 HPO 4 0.2%, c) soybean meal 20%, bran 80%, glucose 1.0%, K 2 HPO 4 0.2%, d) soybean meal 20%, soybean skin 80%, glucose 1.0%, K 2 HPO 4 0.2%, e) full price feed, glucose 1.0%, K 2 HPO 4 0.2%.

[0033] (2) The material-to-water ratio of the above raw materials is 7:3, glucose and K 2 HPO 4 Add water to dissolve, and add feed starter to water according to 5% inoculum amount to obtain mixture A, add A to the fermentation raw material, and stir evenly to obtain mixture B.

[0034](3) Put B into a well-sealed plastic drum or plastic bag, and ferment at room temperature (20-30°C). The feed raw material emits an obvious sour aroma to complete the fermentation. Generally, it only takes 2 days. After fermentation, the...

Embodiment 3

[0039] Usage of Feed Raw Materials After Fermentation

[0040] Method: The experiment selected Penaeus vannamei with normal appearance and strong physique as the experimental shrimp, and each pond (20m 2 ) stocking 5000 Penaeus vannamei prawns, feeding 6% of the body weight every day, and feeding in four times, the control group was fed with conventional feed, and the treatment group was fed with 10% fermented feed added to the conventional feed. During the feeding period, the water temperature was (29±3)°C, and the water was continuously aerated. At the 6th week of the experiment, the intestinal tract was removed, the contents were removed, rinsed with deionized water, blotted dry with filter paper, weighed, crushed, added to 10 mL of sterile normal saline for homogenization, and the total number of bacteria and the number of Vibrio were measured after gradient dilution .

[0041] Results: It can be seen from Table 2 that the total number of bacteria and total number of Vib...

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Abstract

The invention discloses a feed fermentation agent and application in feedstuff fermentation. The feed fermentation agentis formed by mixing the fermentation liquor of candida utilis and lactobacillus plantarum and the like in proportion. Feed raw materials are mixed in proportion, the material-water ratio of the raw materials is 7 to 3, 1% glucose and 0.2 % K2HPO4 are added to the water for dissolution, the feed fermentation agent is added into the water according to 5% inoculation amount, the obtained mixture is added to fermentationraw materials, the mixture is charged into an airtight container after even stirring, room-temperaturefermentation is performed for 2-3 days, and a microbial fermentation feed is obtained. By adopting the method, the feed raw materials having poor palatability and low nutritive values can be fermented into the sour, fragrant and deliciousmicrobial fermentation feed under the conditions of room temperature and opening, the nutrition value and digestion and absorption rate of the feed are improved, and the gut microbial flora of fish and shrimps areadjusted. The method is simple in operating process, low in cost and convenient to popularize in a large-scale mode.

Description

technical field [0001] The invention relates to the field of microorganism application and feed fermentation, in particular to a feed starter composed of Candida utilis and Lactobacillus plantarum and its application in feed raw material fermentation. Background technique [0002] After the feed raw materials are fermented by microorganisms, the protein, fat, carbohydrates and other macromolecular substances in the raw materials are hydrolyzed by microbial enzymes into small molecular substances such as amino acids, fatty acids, sugars, etc., which improves the nutritional value and digestibility of the product; while the raw materials are difficult to digest The flatulent oligosaccharides and anti-nutritional factors can be removed or reduced by fermentation. Therefore, fermented feed has the characteristics of good palatability, high nutritional value, safety, and no pollution. Feeding fermented feed can not only improve the digestion and utilization rate of feed, promote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K20/163A23K20/26A23K50/80C12N1/20C12N1/16C12R1/25C12R1/72
CPCC12N1/16C12N1/20A23V2400/169Y02P60/87
Inventor 谢凤行周可张峰峰赵玉洁赵琼
Owner 天津市农业科学院
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