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Culture medium for improving flavonoid compound and polysaccharide of scutellariae barbatae tissue culture seedling

A technology of flavonoids and tissue culture seedlings is applied in the field of bark lotus planting to achieve the effects of accelerated accumulation, rapid root growth and comprehensive nutrition.

Inactive Publication Date: 2017-07-07
LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a culture medium for increasing the flavonoids and polysaccharides of barbatae tissue culture seedlings to solve the problem of how to increase the content of flavonoids and polysaccharides in the main active ingredients of barbata in order to improve economic benefits in the existing tissue culture medium And other issues

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0088] The preparation method of the culture medium for improving flavonoids and polysaccharides in tissue culture seedlings of Scutellaria barbata comprises the following steps:

[0089] S1: the preparation of ginkgo flavonoids, comprising the following steps:

[0090] S11: crush the dried ginkgo leaves and pass through a 40-80 mesh sieve to obtain ginkgo leaf powder;

[0091] S12: Extract the ginkgo leaf powder prepared in step S11 with water at 62-75°C, the solid-liquid ratio in the extraction is 1:12-16 (W / V), the microwave power is 200-800W, and the extraction time is 15-30min, extract 2-3 times, filter after extraction, and combine the filtrate to obtain a mixed extract;

[0092] S13: Concentrate and dry the mixed extract prepared in step S12 to obtain an extract, which is dissolved in water at 52-55°C with 5.5-6 times the volume of the extract, and extracted with water-saturated n-butanol 2-3 times, continue to carry out multiple crystallization treatments with n-buta...

Embodiment 1

[0098] A culture medium for improving flavonoids and polysaccharides in tissue culture seedlings of Scutellaria barbata, comprising the following raw materials in parts by weight: 42 parts of sugar, 15 parts of ginkgo flavonoids, 2.5 parts of potassium salt, 2 parts of phosphorus salt, nitrogen 1.5 parts of salt, 0.7 parts of calcium salt, 0.7 part of magnesium salt, 03 parts of iodine salt, 0.3 part of boron salt, 0.3 part of molybdenum salt, 0.3 part of zinc salt, 0.3 part of manganese salt, 0.3 part of copper salt, 0.3 part of iron salt, vitamin 0.18 parts, 0.18 parts of amino acids, 0.08 parts of auxin, 0.08 parts of gibberellin, 0.06 parts of succinimide, 0.05 parts of cytokinin, 0.07 parts of kinetin, 0.2 parts of conditioning agent, 1.8 parts of dispersing agent, synergist 0.05 parts, 1.4 parts of stabilizer, 2 parts of antibacterial agent, 2500 parts of water;

[0099] The sugar is glucose;

[0100] The potassium salt is potassium chloride;

[0101] The phosphorus sa...

Embodiment 2

[0129] A culture medium for improving flavonoids and polysaccharides in tissue culture seedlings of Scutellaria barbata, comprising the following raw materials in parts by weight: 32 parts of sugar, 12 parts of ginkgo flavonoids, 2.2 parts of potassium salt, 1.3 parts of phosphorus salt, nitrogen 1.1-2 parts of salt, 0.6 part of calcium salt, 0.6 part of magnesium salt, 0.2 part of iodine salt, 0.2 part of boron salt, 0.2 part of molybdenum salt, 0.2 part of zinc salt, 0.2 part of manganese salt, 0.2 part of copper salt, 0.2 part of iron salt , 0.1 part of vitamin, 0.08 part of amino acid, 0.05 part of auxin, 0.06 part of gibberellin, 0.04 part of succinimide, 0.03 part of cytokinin, 0.04 part of kinetin, 0.15 part of conditioning agent, 1.2 part of dispersing agent, 0.03 parts of effect agent, 0.9 parts of stabilizer, 1.5 parts of antibacterial agent, 2000 parts of water;

[0130] The sugar is glucose;

[0131] The potassium salt is potassium chloride;

[0132] The phosphor...

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PUM

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Abstract

The invention discloses a culture medium for improving a flavonoid compound and polysaccharide of a scutellariae barbatae tissue culture seedling. The culture medium is prepared from the following raw materials: sugar, ginkgo flavone, sylvite, a microcosmic salt, a nitrogen salt, a calcium salt, a magnesium salt, an iodine salt, a boron salt, a molybdenum salt, a zinc salt, a manganese salt, a copper salt, a ferric salt, vitamin, amino acid, auxin, gibberellin, succinimide, cytokinin, kinetin, a conditioner, a dispersing agent, a synergist, a stabilizer, an antibacterial agent and water. The scutellariae barbatae tissue culture seedling is reasonable in collocation and comprehensive in nutrient, roots and stems can be quickly promoted, the transplanting survival rate is improved, and meanwhile, accumulation of the flavonoid compound and the polysaccharide can be promoted, and the economical benefits are improved.

Description

technical field [0001] The invention belongs to the technical field of barbatae planting, and in particular relates to a culture medium for increasing flavonoids and polysaccharides in tissue culture seedlings of barbatae. Background technique [0002] Scutellaria barbate (Scutellaria barbate D.Don) is a plant of the genus Scutellaria baicalensis in the Lamiaceae family. It is also known as Bingtoucao, Hanxincao stenosa, shanbei, toothbrush grass, spoon grass, orchid grass, etc. It belongs to the perennial wild medicine of Lamiaceae. With herbs, the whole herb is used as medicine. Its nature and flavor are pungent, bitter, and cold, and it returns to the lung, liver, and kidney channels. Scutellaria barbata contains a variety of compounds, and flavonoids are its main active ingredients, which have various pharmacological activities such as anti-tumor, anti-oxidation, and anti-bacterial. [0003] Scutellaria barbata is the drug of choice for cancer treatment, and its usage ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 蔡锦源熊建文莫晓宁蒋潮梁豪荣
Owner LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH