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Method for preparing functional active peptide by glutamic acid fermentation solid residues

A functionally active, glutamic acid technology, applied in the fields of fermentation, chemical instruments and methods, food science, etc., can solve the problems of affecting the shelf life of the final product, changing the functional properties of proteins, and having too many unsaturated fats and oils, etc. The effect of using and ensuring safety

Inactive Publication Date: 2017-07-25
WUXI JINNONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzymatic hydrolysis condition is alkaline pH9.5, and the protein may react with alkali to produce carcinogens, which will change the normal functional properties of the protein. Sesame meal is used, that is, the meal after pressing sesame contains more oil components. This point is not taken into account in this paper. The oil components are obtained by drying in the supernatant along with the active peptide. On the one hand, the oil has many unsaturated components, which affects the shelf life of the final product. On the other hand, the existence of oil does not guarantee the purity of the active peptide high

Method used

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  • Method for preparing functional active peptide by glutamic acid fermentation solid residues
  • Method for preparing functional active peptide by glutamic acid fermentation solid residues

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Effect test

Embodiment 1

[0035] (1) Raw material selection and pretreatment: select oily material residues that have no mildew, sour odor and obvious qualitative change after glutamic acid fermentation process, and remove obvious inorganic impurities and organic impurities. Inorganic impurities include soil, sand, gravel, Cinders, bricks and tiles, glass fragments, metals and other inorganic substances, organic impurities include plant roots, stems, hairs, seeds, alien grains, insect pupae, and insect carcasses.

[0036] (2) Pulverization: the solid slag after the pretreatment is sieved after being pulverized in two steps of coarse pulverization and fine pulverization, and the mesh number of the sieve is 60 orders. When a certain amount is reached, the equipment automatically pumps the material in the temporary storage tank of the sieve material into the coarse crushing feed port and pulverizes the new material at the same time. The content of fiber substances in the sieve material is determined to be 62...

Embodiment 2

[0041] (1) Raw material selection and pre-treatment: select processed potato slag that has no mildew, sour odor and obvious qualitative change, and has been processed by glutamic acid fermentation process to remove obvious inorganic impurities and organic impurities. Inorganic impurities include soil, Sand, gravel, cinders, bricks, glass fragments, metal objects and other inorganic substances, organic impurities include plant roots, stems, hairs, seeds, foreign seeds, pupae, and insect corpses.

[0042] (2) Pulverization: the solid slag after the pretreatment is sieved after being pulverized in two steps of coarse pulverization and fine pulverization, and the mesh number of the sieve is 80 orders. When a certain amount is reached, the equipment automatically pumps the material in the temporary storage tank of the sieve into the coarse crushing feed port and crushes the new material at the same time. The fiber content in the sieve is detected to be 71%, and the fineness of the m...

Embodiment 3

[0047] (1) Raw material selection and pre-treatment: select the slag processed by the glutamic acid fermentation process without mildew, sour smell and obvious qualitative change, and remove obvious inorganic impurities and organic impurities. Inorganic impurities include soil, Sand, gravel, cinders, bricks, glass fragments, metal objects and other inorganic substances, organic impurities include plant roots, stems, hairs, seeds, foreign seeds, pupae, and insect corpses.

[0048] (2) Pulverization: the solid slag after the pretreatment is sieved after being pulverized in two steps of coarse pulverization and fine pulverization, and the mesh number of the sieve is 70 orders. When a certain amount is reached, the equipment automatically pumps the material in the temporary storage tank of the sieve into the coarse crushing feed port and crushes the new material at the same time. The fiber content in the sieve is detected to be 65%, and the fineness of the material after sieving is...

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Abstract

The invention relates to a method for preparing functional active peptide by glutamic acid fermentation solid residues. The method is characterized by comprising the following steps of (1) selecting and pretreatment of a raw material; (2) crushing and separating of slurry and residues; (3) removing of impurities (besides protein) by a chemical and physical combining method; (4) directional hydrolyzing reaction of two types of protease; (5) concentrating and drying of an enzyme hydrolyzing material, so as to obtain the finished active peptide. The molecular weight distribution ratio of active peptide at 500 to 1500 Da is greater than 60%; by measuring the ability of removing DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, the anti-oxidizing activity is 50% to 70%. The active peptide can be used as a protein nutrient supplementing agent in food or reach the anti-oxidizing function, and can be used as an organic nitrogen source in organic fertilizer for plants to directly absorb and utilize, or be combed with some metal elements in soil to improve soil quality.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for preparing functional active peptides from solid residues after glutamic acid fermentation. Background technique [0002] Glutamate fermentation bacteria residue is the main by-product of the monosodium glutamate industry, with an annual output of millions of tons. In the past, these glutamic acid fermentation bacteria residues were mainly discharged through wastewater, which not only caused environmental pollution but also wasted resources. The comprehensive development of monosodium glutamate industrial by-products and the realization of all-round utilization of food resources have important practical significance. According to the biological evaluation standard, the bacteria producing MSG and all the biosynthetic products are harmless. It is an underutilized renewable resource and is currently only sold as feed in most enterprises in our country. [0003...

Claims

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Application Information

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IPC IPC(8): C12P21/06C05C11/00C09K17/14A23L33/18
CPCC05C11/00C09K17/14C12P21/06
Inventor 于秋生李珍妮陈林冯伟陈天祥朱熹
Owner WUXI JINNONG BIOTECH CO LTD
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