Bacillus amyloliquefaciens HMB28388 and application thereof

A technology of HMB28388 and amylolytic spores, applied in application, bacteria, fungicides, etc., can solve the problems of new diseases or secondary diseases, difficulty in cultivating resistant varieties, less understanding of resistant materials, etc., and achieve high efficacy , It is not easy to produce drug resistance, and the effect of drug durability is good

Active Publication Date: 2017-08-15
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The control methods for cucumber target spot disease include: (1) Cultivate disease-resistant varieties: cultivating disease-resistant varieties is the most economical and effective method for disease control, but due to poor understanding of cucumber target spot disease-resistant materials in domestic cucumber germplasm resources Less, it is difficult to breed resistant varieties
(2) Prevention and control of agricultural cultivation measures: such as reducing planting density, timely cleaning of sick and old plants and leaves, using drip irrigation under mulch to reduce greenhouse humidity, etc., but the control effect is poor
However, the long-term use of chemical pesticides in large quantities will easily cause pathogenic bacteria to develop drug resistance, resulting in reduced control effects; at the same time, it will easily cause agricultural products and environmental pollution, causing new diseases or secondary diseases to occur

Method used

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  • Bacillus amyloliquefaciens HMB28388 and application thereof
  • Bacillus amyloliquefaciens HMB28388 and application thereof
  • Bacillus amyloliquefaciens HMB28388 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of embodiment 1HMB28388 microbial bacterial agent

[0051] Follow the steps below:

[0052] (1) Strain activation: The Bacillus amyloliquefaciens strain HMB28388 stored at -80°C (the strain HMB28388 had been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on October 28, 2016, and the preservation number was CGMCC No. .13210) was activated (30°C) on LB plate culture medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), pick A single colony was cultured at 30°C for 30 hours on LB slant medium (its composition and weight ratio: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) to obtain activated the strain;

[0053] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium ch...

Embodiment 2

[0056] Embodiment 2 Antagonism test of HMB28388 bacterial strain of the present invention to cucumber target spot bacterium

[0057] (1) Source of Cucumber target spot fungus CA-1 strain: Cucumber target spot spot fungus CA-1 strain was collected from cucumber diseased leaves in Da Da Village, Mancheng County, Baoding City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The Department of Plant Pathology, School of Plant Protection, Hebei Agricultural University identified it as Corynespora cassiicola, and the pathogenicity test showed strong pathogenicity.

[0058] (2) Test method:

[0059] Plate stand-off experiment: First, activate and culture the CA-1 strain of Cucumber spp. The sheet is transferred in the center of another PDA plate; the Bacillus amyloliquefaciens HMB28388 activated in the step (1) of Example 1 is connected to 2.0 centimeters away from the indicator bacteria sheet, an...

Embodiment 3

[0064] Example 3 Comparison test of the control effect of HMB28388 bacterial strain of the present invention on cucumber target spot disease

[0065] (1) Test treatment:

[0066] (1) HMB28388 microbial bacterial agent: 100 times water dilution of the HMB28388 liquid preparation prepared in Example 1;

[0067] (2) Blank control: clear water.

[0068] (2) Test method:

[0069] This experiment was carried out in the plant disease and biocontrol laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0070] (1) Cultivate cucumber plants: Cultivate Jinyou No. 1 cucumber seedlings in a nutrient bowl with a diameter of 6 cm. The seedling substrate is vermiculite. Two seeds are sown in each nutrient bowl, and one strong seedling is retained after emergence. When the cucumber seedlings grow two true leaves, they are transplanted into plastic flower pots with a diameter of 25 cm (the flower pots are filled with a substrate made of vermic...

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Abstract

The invention discloses a bacillus amyloliquefaciens HMB28388 strain. The strain is collected in CGMCC, and the collection number is CGMCC No.13210. The invention also discloses a microbial inoculant produced by means of the strain and an application of the strain in preventing and treating plant diseases such as cucumber target spot. The HMB28388 strain disclosed by the invention is high in efficacy on the cucumber target spot, and the average control effect is 90.0% above. Secondly, drug resistance is unlikely to generate by using the strain to prevent and treat the cucumber target spot, and no environmental pollution problems are available; the strain is simple to prepare and use and low in cost; in addition, the HMB28388 strain is wide in antibacterial spectrum. Besides the cucumber target spot, the HMB28388 strain also has very good inhibiting action on verticillium wilt of cotton, cotton-wilt fusarium, rhizoctonia solani of cotton or botrytis cinerea.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a bacillus amyloliquefaciens HMB28388, and also relates to microbial agents produced by the bacillus amyloliquefaciens, and their use in preventing and treating plant diseases such as cucumber target spot disease. Background technique [0002] Cucumber target spot disease (also known as Corynespora leaf spot, brown spot, "yellow spot disease", etc.) is caused by Corynespora cassiicola (Berk & Curt) Wei., especially in wintering greenhouses, winter-spring greenhouses, Serious occurrences occurred in protected areas such as spring greenhouses. The symptoms are yellow water-soaked spots with a diameter of about 1 mm at the beginning; in the middle stage of the disease, the lesions expand to round or irregular shapes, and are easy to perforate. The lesions on the front of the leaves are rough and uneven. The diameter of the lesion can reach 10-15 mm, and there is an obv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 鹿秀云马平李社增郭庆港张晓云丛蓉王培培赵卫松
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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