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Bacillus licheniformis and separation method and application thereof

The technology of Bacillus licheniformis and bacterial agent is applied in the field of microbial application and feed processing and production, which can solve the problems of difficult to withstand high temperature of feed, few microbial preparations, enzyme production, and poor bacteriostatic ability, etc. The effect of improving feed utilization

Active Publication Date: 2017-08-15
SHENYANG BOYANG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the key issues in the research and development of probiotics are the isolation and screening of excellent strains, and a good probiotic treatment process.
At present, the following problems exist in probiotic strains: 1. Most probiotics are lactic acid bacteria that do not produce spores, and most of them are anaerobic bacteria, which are easily affected by external environmental factors such as temperature, oxygen, moisture, and acid-base. Generally, it is difficult to maintain the activity of probiotics; 2. Most probiotics are difficult to withstand the high temperature during feed pelleting during feed processing; 3. The characteristics of the strain itself are not good, and the ability to produce enzymes and inhibit bacteria is poor
However, there are few studies on the application of microbial preparations in ruminant nutrition and feed, especially the application of microbial probiotics in deer nutritional feed

Method used

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  • Bacillus licheniformis and separation method and application thereof
  • Bacillus licheniformis and separation method and application thereof
  • Bacillus licheniformis and separation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The present embodiment provides the isolation and identification of Bacillus licheniformis, and the specific operations are as follows:

[0048] 1.1 Take 20g of rumen juice of sika deer and add it to 100ml of sterile saline;

[0049] 1.2 Shake the shaker for 30 minutes, fully dissolve and mix;

[0050] 1.3 In a water bath at 85°C for 15 minutes, according to 0, 10, 10 2 、10 3 、10 4 、10 5 、10 6 、10 7 、10 8 and 10 9 A total of 10 gradients were diluted;

[0051]1.4 Use the pouring culture method to isolate the cultured bacterial liquid, and culture it at 37°C for 48 hours;

[0052] 1.5 Pick a single spot with an inoculation loop, inoculate it on a plate by streaking on a plate, and carry out purification and culture;

[0053] 1.6 Repeat step 1.5 to continuously purify for 4 generations to obtain Bacillus licheniformis and biologically pure cultures of Bacillus licheniformis.

[0054] The morphological observation of the purified Bacillus licheniformis found tha...

Embodiment 2

[0056] The present embodiment provides a kind of Bacillus licheniformis, and the basic information of this Bacillus licheniformis is: Classification name: Bacillus licheniformis; On May 20, 2016, it was located at No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China. It is preserved by the General Microbiology Center of the China Microbiological Culture Collection Management Committee of the Institute of Microbiology, Academy of Sciences, and the preservation number is CGMCC No.12486.

Embodiment 3

[0058] This embodiment provides a bacterial agent and feed, which include the Bacillus licheniformis provided in Example 2.

[0059] The preparation method of this bacterial agent is as follows:

[0060] 1.1 prepare the fermentation medium of Bacillus licheniformis, formula is: brown sugar 1.8% (m / V), corn flour 1% (m / V), bean dregs 2.4% (m / V), ammonium citrate 1.8% (m / V) V), 0.6% (V / V) of mixed salt solution, 0.08% (V / V) of defoamer, are made into fermentation medium in proportion;

[0061] 1.2 Then adjust the pH to 7.2, and sterilize with high-temperature steam at 121°C for 20 minutes;

[0062] 1.3 When the temperature is lowered to 37°C, inoculate the fermentation medium with 3.5% (V / V) seed solution of 20 hours old;

[0063] 1.4 Under the condition of 37°C, keep stirring at 250rpm, ferment and cultivate for 18 hours, put it into the tank, and the number of viable Bacillus licheniformis is 1.39×10 11 cfu / mL.

[0064] A protective agent (0.8% of glycerol, 0.2% of sodium ...

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Abstract

The invention provides Bacillus licheniformis and a separation method and an application thereof, which belong to the fields of microbe application and feed processing production. The Bacillus licheniformis can effectively increase at least one of cellulose, amylase or pepsin in artiodactyla animal gastric juice, the above Bacillus licheniformis is used for preparing inocula or a feed, and can better improve the problems of low utilization rate and slow growth of the feed in an economic animal breeding process, the above inocula or the feed can increase the utilization rate of an artiodactyla animal feed, which can reach a purpose of rapid growth.

Description

technical field [0001] The invention relates to the field of microorganism application and feed processing production, in particular to a bacillus licheniformis and its isolation method and application. Background technique [0002] With the improvement of people's living standards and the increasing emphasis on health, food safety has also received more and more attention from everyone. [0003] As a supplier of animal food, animal husbandry plays an important role in maintaining food safety. In my country's aquaculture industry, the excessive use of antibiotics has caused drug residues and a large number of drug-resistant bacteria. There are hidden dangers, so it is imminent to find safe, residue-free alternatives to antibiotics. Biological feed additives containing probiotics are an ideal substitute for some antibiotics, and probiotics are an important basis for developing biological feed additives with stable quality, significant effects, and high cost performance. How...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A23K10/18C12R1/10
CPCC12N1/02C12N1/20C12N1/205C12R2001/10
Inventor 刘晓颖李光玉王卓刘晗璐鲍坤王晓旭
Owner SHENYANG BOYANG FEED
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