Lysozyme cross-linked dextran sustained-release microspheres and preparation method thereof

A technology of cross-linked dextran and slow-release microspheres, which is applied in antibacterial drugs, pharmaceutical formulas, medical preparations of non-active ingredients, etc., to achieve strong biosorption capacity, strong water absorption performance, and significant inhibition effects

Inactive Publication Date: 2017-08-18
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Lysozyme cross-linked dextran sustained-release microspheres and preparation method thereof
  • Lysozyme cross-linked dextran sustained-release microspheres and preparation method thereof
  • Lysozyme cross-linked dextran sustained-release microspheres and preparation method thereof

Examples

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Embodiment 1

[0021] Example 1 Preparation of lysozyme cross-linked dextran sustained-release microspheres

[0022] Prescription: Lysozyme 0.05g

[0023] Cross-linked dextran microspheres 1.0g

[0024] Purified water 10ml

[0025] Preparation method: Dissolve the prescribed amount of lysozyme in purified water in a clean area, add the prescribed amount of cross-linked dextran microspheres, stir at room temperature until the microspheres are completely dispersed, and let stand for 4 hours for adsorption and loading. The microspheres were collected by filtration and vacuum freeze-dried to obtain lysozyme cross-linked dextran sustained-release microspheres with a drug loading of 2.85%.

Embodiment 2

[0026] Example 2 Preparation of lysozyme cross-linked dextran sustained-release microspheres

[0027] Prescription: Lysozyme 0.225g

[0028] Cross-linked dextran microspheres 1.0g

[0029] Purified water 15ml

[0030] Preparation method: Dissolve the prescribed amount of lysozyme in purified water, add the prescribed amount of cross-linked dextran microspheres, stir at room temperature until the microspheres are completely dispersed, and let stand for 12 hours to absorb and load the drug. The microspheres were collected by filtration and vacuum freeze-dried to obtain lysozyme cross-linked dextran sustained-release microspheres with a drug loading of 7.80%.

Embodiment 3

[0031] Example 3 Preparation of lysozyme cross-linked dextran sustained-release microspheres

[0032] Prescription: Lysozyme 0.5g

[0033] Cross-linked dextran microspheres 2.0g

[0034] Purified water 20ml

[0035] Preparation method: Dissolve the prescribed amount of lysozyme in purified water, add the prescribed amount of cross-linked dextran microspheres, stir at room temperature until the microspheres are completely dispersed, and let stand for 24 hours to absorb and load the drug. Microspheres were collected by filtration, and vacuum freeze-dried to obtain lysozyme cross-linked dextran sustained-release microspheres (see figure 1 ), the drug loading is 13.25%, the average particle diameter is 207um, and the swelling degree is 8.9ml / g.

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Abstract

The invention belongs to the field of drug delivery systems of macromolecular drugs and in particular relates to lysozyme cross-linked dextran sustained-release microspheres and a preparation method thereof. The lysozyme cross-linked dextran sustained release microspheres are characterized by being prepared from the following components: cross-linked dextran microspheres and lysozyme. The preparation method comprises the following steps: weighing quantitative lysozyme and adding the lysozyme into purified water; after the lysozyme is completely dissolved, adding quantitative dextran microspheres into a solution; sufficiently stirring on a magnetic stirrer to completely disperse the microspheres; standing at room temperature and carrying out adsorption and drug loading; filtering and collecting the microspheres; freezing and drying in vacuum to obtain the lysozyme cross-linked dextran sustained-release microspheres. The sustained-release microspheres provided by the invention have good sustained-release effect, water swelling effect and biological adhesion effect and can be used for mucosal drug delivery and skin drug delivery so as to better express anti-inflammatory, antibacterial and anti-virus effects of the lysozyme.

Description

technical field [0001] The invention relates to the field of drug delivery systems for macromolecular drugs, in particular to a lysozyme cross-linked dextran sustained-release microsphere and a preparation method thereof. Background technique [0002] Lysozyme (lysozyme), also known as cell wall lytic enzyme, is a hydrolase that specifically acts on the cell wall of microorganisms and can cut off the peptidoglycan between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG). β-1,4 glycosidic bond, destroys the peptidoglycan scaffold, and the cell ruptures under the action of internal osmotic pressure, causing cell lysis. Lysozyme is an effective antibacterial agent. The enzyme has a good inhibitory effect on Bacillus subtilis, Proteus vulgaris and Vibrio parahaemolyticus among Gram-positive bacteria, and it also dissolves Gram-negative bacteria to a certain extent. effect. In addition, lysozyme also has the effect of anti-virus and enhancing immunity. In medical treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/61A61K38/47A61P29/00A61P31/04A61P31/12
CPCA61K9/1652A61K38/47
Inventor 栾立标唐小康
Owner CHINA PHARM UNIV
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