Application of pedicel glucoside in the preparation of anti-influenza virus drugs
A technology of leucoside and influenza virus, which is applied in the directions of antiviral agents, drug combinations, pharmaceutical formulations, etc., to achieve the effect of reducing lung index
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Embodiment 1
[0014] Example 1 The preparation of the extract of medicinal materials
[0015] Get an appropriate amount of Bing medicinal materials, crush them, pass through a No. 1 sieve, add 8 times the amount of 70% (volume fraction) ethanol to the medicinal materials, heat and reflux for extraction twice, each time for 1 hour, filter, combine the extracts, put them in a rotary evaporator, Concentrate under reduced pressure at 80°C to a thick paste. Transfer the concentrated solution to a decompression drying oven, continue drying and crushing at 80°C, and obtain the rescued Bing medicinal material extract (detected by liquid chromatography, the mass fraction of pedicel glucoside in the extract is 200 mg / g).
Embodiment 2
[0016] Example 2 In vitro Influenza Virus Inhibition Experiment
[0017] 1. Experimental materials
[0018] 1.1 Tested drug: pedicel glucoside (extracted from jiubing, purity > 98%).
[0019] 1.2 Control drug: ribavirin, oleanolic acid, extract of the medicinal material of Qubing (see Example 1)
[0020] 1.3 Virus strain: Influenza A H1N1 influenza virus murine lung adaptation strain (FM / 1 / 47 strain). Passage chicken embryos, test in BSL-3 (Biosafety Laboratory Level 3), subpackage, and store at -80°C.
[0021] 1.4 Cell model: dog kidney cell MDCK cell
[0022] The above drugs were fully dissolved in dimethyl sulfoxide (DMSO) and stored at -20°C for later use.
[0023] 2. Experimental method
[0024] 2.1 Determination of virus virulence
[0025] 100 μl of 10-concentration virus solutions obtained by 10-fold serial dilution were set up in 8 replicate wells for each concentration, and were sequentially inoculated into a 96-well plate covered with a single layer of MDCK cel...
Embodiment 3
[0034] Example 3 In vivo Influenza Suppression Experiment
[0035] 1. Experimental materials
[0036] 1.1 Tested drug: pedicel glucoside (extracted from jiubing, purity > 98%).
[0037] 1.2 Control drug: Tamiflu, oleanolic acid, extract of the medicinal material of Qubing (Example 1)
[0038] 1.3 Virus: Mouse lung-adapted strain of influenza A H1N1 virus (FM / 1 / 47 strain). Passage chicken embryos, test in BSL-3 (Biosafety Laboratory Level 3), subpackage, and store at -80°C.
[0039] 2. Experimental animals
[0040] 80 BALB / c mice weighing 18g-22g were purchased from Guangdong Experimental Animal Center.
[0041] 3. Experimental method
[0042] The BALB / c mice were randomly divided into 8 groups, namely, the blank control group, the model control group, the Tamiflu control group, the oleanolic acid group, the medicinal material extract group of Jubiying, and the high, medium and low levels of pedicel glucoside. 3 dosage groups, 10 rats in each group. Except for the blank ...
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