Preparation method of immobilized anammox bacterial sludge and method for sewage treatment with the same

An anammox bacteria and sewage treatment technology, applied in anaerobic digestion treatment, water/sludge/sewage treatment, biological water/sewage treatment, etc., can solve problems such as poor treatment effect and easy loss of bacterial sludge. Achieve the effect of increasing the retention time of bacterial sludge, improving the microenvironment, and being easy to obtain

Active Publication Date: 2017-09-15
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Aiming at the problem that the bacteria sludge in the existing traditional anammox treatment is easy to lose and the treatment effect is not good, the present invention provides a method of combining bacteria sludge embedding and fixation with magnetite filler to prevent anammox bacteria from At the same time, under the action of sulfur autotrophic denitrifying bacteria, the nitrate nitrogen in the water body is reduced to N 2 , to further improve the water quality

Method used

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  • Preparation method of immobilized anammox bacterial sludge and method for sewage treatment with the same
  • Preparation method of immobilized anammox bacterial sludge and method for sewage treatment with the same

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Take by weighing the polyvinyl alcohol solution that mass fraction is 15%, the gel-type glucomannan gum that mass fraction is 3%, the Fe that mass fraction is 6%. 3 o 4 Powder, protein powder with a mass fraction of 3%, was added to distilled water with a mass fraction of 73%, mixed and stirred evenly to make a fixative solution.

[0043] The anammox bacteria slime was washed with acetone, ethanol, and potassium bicarbonate solution in sequence, and then the granular anammox bacteria sludge was dispersed into a uniform floc with a homogenizer, and then the prepared fixing solution was mixed with The bacteria slime flocs of equal volume are uniformly mixed, the mass concentration of the potassium bicarbonate solution used is 2%, and the pH value is about 8.

[0044] The resulting mixture was delivered to 6% by mass CaCl with a peristaltic pump at a flow rate of 20 mL / min. 2 solution, and then stored at 0° C. for 1 to 2 days at a low temperature, finally forming gel bea...

Embodiment 2

[0050] Take by weighing the polyvinyl alcohol solution that mass fraction is 10%, the gel-type glucomannan gum that mass fraction is 3%, the Fe that mass fraction is 4% 3 o 4 Powder, protein powder with a mass fraction of 1%, was added to distilled water with a mass fraction of 82%, mixed and stirred evenly to make a fixative solution.

[0051] The anammox bacteria slime was washed with acetone, ethanol, and potassium bicarbonate solution in sequence, and then the granular anammox bacteria sludge was dispersed into a uniform floc with a homogenizer, and then the prepared fixing solution was mixed with The bacteria slime flocs of equal volume are uniformly mixed, the mass concentration of the potassium bicarbonate solution used is 2%, and the pH value is about 8.

[0052] The resulting mixture was delivered to 4% by mass CaCl with a peristaltic pump at a flow rate of 20 mL / min. 2 solution, and then stored at 0° C. for 1 to 2 days at a low temperature, finally forming gel bead...

Embodiment 3

[0058] Take by weighing the polyvinyl alcohol solution that mass fraction is 12%, the gel-type glucomannan gum that mass fraction is 3%, the Fe that mass fraction is 5%. 3 o 4 Powder, protein powder with a mass fraction of 2%, was added to distilled water with a mass fraction of 78%, and then stirred evenly to make a fixative solution.

[0059] The anammox bacteria slime was washed with acetone, ethanol, and potassium bicarbonate solution in sequence, and then the granular anammox bacteria sludge was dispersed into a uniform floc with a homogenizer, and then the prepared fixing solution was mixed with The bacteria slime flocs of equal volume are uniformly mixed, the mass concentration of the potassium bicarbonate solution used is 2%, and the pH value is about 8.

[0060] The resulting mixture was delivered to 5% by mass CaCl with a peristaltic pump at a flow rate of 20 mL / min. 2 solution, and then stored at 0° C. for 1 to 2 days at a low temperature, finally forming gel bead...

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Abstract

Belonging to the field of biological sewage treatment, the invention discloses a preparation method of immobilized anammox bacterial sludge and a method for sewage treatment with the same. According to the invention, polyvinyl alcohol, konjac glucomannan, protein powder, Fe3O4 and anammox bacterial sludge are mixed to prepare immobilized particles, then magnetic pyrite particles are added into a fluidized bed reactor, and are mixed with the immobilized particles to serve as biological filler. Through magnetic adsorption of magnetic pyrite to Fe3O4, loss of embedded particles can be avoided, anammox bacterial colonies can adhere to the filler stably, and the residence time of anammox bacteria can be increased, thus improving the removal efficiency of ammonia nitrogen and nitrite nitrogen in wastewater, at the same time sulfur in the magnetic pyrite can be used as a denitrification electron donor, nitrate nitrogen in the water can be reduced into N2 through the action of autotrophic denitrification bacteria, thus enhancing the overall nitrogen removal effect. The immobilized anammox bacterial sludge prepared by the method provided by the invention has the advantages of simple preparation, low cost, good treatment effect and strong shock resistance, and is suitable for practical production.

Description

technical field [0001] The invention belongs to the field of sewage biological treatment, and more specifically relates to a preparation method of immobilized anammox bacteria sludge and a method for sewage treatment thereof. Background technique [0002] Anammox (Anammox) is a biological denitrification technology that has attracted much attention in recent years. Its powerful function comes from ammonium oxidizing bacteria, a type of bacteria belonging to Planctomycetes. Its cells are divided into 3 parts: Anammox body, ribose cytoplasm and exosome cytoplasm. Among them, the anammox body is a unique structure of anammox bacteria, accounting for 50% to 80% of the cell volume, and the main reaction of anammox is carried out in it, that is, under anaerobic conditions, NO 2 - Utilize NH 4 + Reacts as an electron donor to generate N 2 and a small amount of NO 3 - , because this process does not require an external carbon source, which greatly saves resources and reduces ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28
CPCC02F3/2806C02F3/2846C02F2203/006
Inventor 刘波盛世雄杜凌峰孙信伯梁子
Owner NANJING UNIV
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