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Application of Corylifol A in preparing anti-radiation drug

An anti-radiation and drug technology, applied in the field of anti-radiation medicine, can solve the problems of weak radiotherapy selectivity, affecting the treatment effect of tumor patients, and treatment failure.

Active Publication Date: 2017-10-24
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the selectivity of radiotherapy is weak, and the radiation can cause damage to normal tissues while irradiating tumor cells, and even cause severe toxic side effects.
Clinically, treatment failure is often caused by the limitation of normal tissue tolerance dose, which seriously affects the treatment effect of tumor patients.

Method used

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  • Application of Corylifol A in preparing anti-radiation drug
  • Application of Corylifol A in preparing anti-radiation drug
  • Application of Corylifol A in preparing anti-radiation drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Extraction of Corylifol A and its characterization

[0029] Extraction of Corylifol A

[0030] 15 kg of psoralen fruit, crushed, soaked and extracted with 75% ethanol at room temperature for 3 times, each time for 24 to 36 hours, concentrated under reduced pressure to obtain 5 L of extract, and the extract was applied to silica gel column chromatography, with a volume ratio of 100:1 Gradient elution to 0:100 petroleum ether-ethyl acetate system to obtain 259g of extract at 5:5; the above extract was subjected to silica gel column chromatography, and dichloromethane- Gradient elution with ethyl acetate system, detection by thin layer chromatography, 5 g of fractions containing Corylifol A were collected, and then preparative liquid chromatography was eluted with a methanol-water system with a volume ratio of 75:25 to 95:5 to obtain Pure Corylifol A 1.6g.

[0031] Characterization of Corylifol A

[0032] Pale yellow powder, by electrospray ion mass spectromet...

Embodiment 2

[0034]Example 2 In vitro cytotoxicity test of Corylifol A

[0035] 2.1 Cell culture

[0036] HBL-100 cells (ATCC) in RPMI-1640 medium (containing 10% fetal bovine serum, 100U / mL penicillin and 0.1mg / mL streptomycin), MCF-7 cells (ATCC) in DMEM / High Gluose medium (containing 10% fetal bovine serum, 100 U / mL penicillin and 0.1 mg / mL streptomycin), at 37°C, 5% CO 2 , cultured in an incubator with saturated humidity. When the cells adhere to the wall and grow to a density of 70-80%, they are subcultured at a ratio of 1 / 2. The number of cells in good growth phase was used for experiments.

[0037] 2.2 Cytotoxicity experiment

[0038] CCK-8 method was used to test the effect of test drugs on the viability of HBL-100 and MCF-7 cells.

[0039] (1) Cell inoculation: cells in good logarithmic growth phase were taken, digested with 0.25% trypsin and prepared into cell suspension, and 7×10 3 Cells / well, 100 μL of cell suspension was uniformly inoculated in a 96-well plate (the periph...

Embodiment 3

[0047] Example 3 Cell Anti-radiation Experiment of Corylifol A

[0048] 3.1 Cell radiation resistance experiment

[0049] Under 2.2 of Example 2, under the condition that there is no significant difference in cell viability between the corylifol A group and the control group, 25 μmol / L was selected to test its radiation damage repairing effect on HBL-100 and MCF-7 cells. The experiment was divided into blank group (no irradiation, no administration), control group (irradiation, no administration), and corylifol A group (ie, experimental group, irradiation, administration).

[0050] Get the cells with good logarithmic growth phase cultured in 2.1 of Example 2, digest them with 0.25% trypsin and prepare a cell suspension, and prepare 7×10 3 1 / well, 100 μL of cell suspension was uniformly inoculated in a 96-well plate (the periphery of the experimental well was filled with sterile PBS); after culturing in the incubator for 12 h, the 96-well plate was taken out, and 100 μL of the...

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Abstract

The embodiment of the invention firstly provides an application of Corylifol A in preparing an anti-radiation drug, wherein the Corylifol A can realize radiation resistance by repairing the cells injured by radiation. The invention also provides an anti-radiation drug compound which contains Corylifol A and a pharmaceutically acceptable carrier or excipient. The invention also provides another anti-radiation drug compound which contains the Corylifol A-containing plant extract and a pharmaceutically acceptable carrier or excipient.

Description

technical field [0001] The invention relates to the technical field of anti-radiation medicine, in particular to the application of Corylifol A in the preparation of anti-radiation medicine. Background technique [0002] In today's society, with the rapid development of the national economy and the continuous improvement of science and technology, radiation has been widely used in various fields. For example, nuclear energy and nuclear technology have been widely used in agriculture, medical care and scientific research. Therefore, the relationship between radiation and people's daily work and life is getting closer and closer. However, when radiation is widely used, it also brings a series of hazards that cannot be ignored, such as: nuclear power radiation, X-rays in the medical field, and nuclear accidents. [0003] Cancer is a major problem in today's medical field. In 2014, the World Health Organization pointed out in the "World Cancer Report" that in the next 20 years,...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P39/00
CPCA61K31/352A61K36/487
Inventor 柴欣高秀梅王春华贺欣王跃飞李正李丛丛杜婕
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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