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A colorimetric detection probe for detecting terramycin in food and a detecting method thereof

A detection probe, oxytetracycline technology, applied in the colorimetric detection probe field of oxytetracycline, can solve problems such as enzyme activity interference, trace presence, matrix interference, etc., and achieve low detection limit, good specificity and selection The effect of sex, good application and development prospects

Inactive Publication Date: 2017-11-10
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common colorimetric method uses horseradish peroxidase to catalyze the color development of some substrates to achieve target detection, but the activity of the enzyme is very susceptible to interference from external conditions.
Therefore, it is very necessary to establish a colorimetric method without enzyme-catalyzed substrate color development. At the same time, there are many detection difficulties in the detection of actual samples, trace amounts exist and matrix interference is serious. A strong colorimetric strategy is still necessary

Method used

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  • A colorimetric detection probe for detecting terramycin in food and a detecting method thereof
  • A colorimetric detection probe for detecting terramycin in food and a detecting method thereof
  • A colorimetric detection probe for detecting terramycin in food and a detecting method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] like figure 1 As shown, this embodiment includes the following steps:

[0032] (1) Preparation of MOF-Pt composite material: first synthesize Fe-MIL-88, weigh 0.118g terephthalic acid and 0.187g FeCl respectively 3 ·6H 2O was dissolved in 15mL of DMF, then 3.45mmol of acetic acid solution was added, reacted in an oil bath at 110°C for 3h, cooled to room temperature to collect the precipitate; to synthesize Pt-PVP, 16.6mg of PVP was dissolved in 45mL of ethanol, and then 5mL of chloroplatinic acid was added dropwise, Then the reaction was stirred at room temperature for 2 min, and finally refluxed for 3 h to collect the precipitated Pt NPs-PVP; finally, the Pt NPs-PVP was added dropwise to the Fe-MIL-88 solution under vigorous stirring, and the Pt NPs-PVP and Fe-MIL The ratio of -88 was 1:1, reacted at room temperature for 2 hours, and finally collected the precipitate by centrifugation to obtain the MOF-Pt composite material.

[0033] (2) Preparation of composite pro...

Embodiment 2

[0045] like figure 1 As shown, this embodiment includes the following steps:

[0046] (1) Preparation of MOF-Pt composite material: first synthesize Fe-MIL-88, weigh 0.354g terephthalic acid and 0.561g FeCl respectively 3 6H 2 O was dissolved in 45mL of DMF, then 7.20mmol of acetic acid solution was added, the oil bath was reacted at 120°C for 4h, and the precipitate was collected after cooling to room temperature; to synthesize Pt-PVP, 33.2mg of PVP was dissolved in 60mL of ethanol, and then 10mL of chloroplatinic acid was added dropwise. Then the reaction was stirred at room temperature for 5 min, and finally refluxed for 5 h to collect the precipitated Pt NPs-PVP; finally, the Pt NPs-PVP was added dropwise to the Fe-MIL-88 solution under vigorous stirring, and the Pt NPs-PVP and Fe-MIL The ratio of -88 was 1:2, reacted at room temperature for 5 hours, and finally collected the precipitate by centrifugation to obtain the MOF-Pt composite material.

[0047] (2) Preparatio...

Embodiment 3

[0059] like figure 1 As shown, this embodiment includes the following steps:

[0060] (1) Preparation of MOF-Pt composite material: first synthesize Fe-MIL-88, weigh 0.251g terephthalic acid and 0.356g FeCl 3 6H 2 O was dissolved in 30mL of DMF, then 5.20mmol of acetic acid solution was added, the oil bath was reacted at 115°C for 4h, and the precipitate was collected after cooling to room temperature; to synthesize Pt-PVP, 20.2mg of PVP was dissolved in 45mL of ethanol, and then 6mL of chloroplatinic acid was added dropwise. Then stir the reaction at room temperature for 3 min, and finally reflux for 3 h to collect the precipitated Pt NPs-PVP; finally, add the Pt NPs-PVP dropwise to the Fe-MIL-88 solution under vigorous stirring, Pt NPs-PVP and Fe-MIL The ratio of -88 was 1:1.5, reacted at room temperature for 3 hours, and finally collected the precipitate by centrifugation to obtain the MOF-Pt composite material.

[0061] (2) Preparation of composite probe solution: Apt ...

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Abstract

The invention relates to a colorimetric detection probe for detecting terramycin in food. A preparing method of the probe includes (1) bonding an aminated aptamer Apt and aminated magnetic beads MB under a function of a coupling agent to form a capturing probe; (2) modifying the surface of a synthesized metal-organic framework MOF-Pt composite material with single stranded DNA binding protein SSB to form a signal probe; and (3) preparing the capturing probe obtained in the step (1) and the signal probe obtained in the step (2) into a composite probe through affinity between the single stranded DNA binding protein and the aptamer Apt. A terramycin detecting method utilizing the probe is also disclosed. The detecting method does not depend on large-scale instruments and has a low detection limit. In addition, the colorimetric detection probe shows good specificity and selectivity. The detecting method is simple and rapid to operate, and can achieve on-site terramycin residue detection by virtue of a handheld chromometer, and an experiment result is visual by naked eyes.

Description

technical field [0001] The invention relates to the fields of environmental science, laboratory chemistry and analytical chemistry, in particular to a colorimetric detection probe for detecting oxytetracycline in food based on an Escherichia coli exonuclease I-assisted target cycle amplification colorimetric strategy and its Detection method. Background technique [0002] Oxytetracycline (OTC) is a broad-spectrum bacteriostatic agent. Many Rickettsia, Mycoplasma, Chlamydia, Spirochetes, Amoeba and some Plasmodium are also sensitive to this product. The mechanism of action of this product is The drug can specifically bind to the A position of the 30S subunit of the bacterial ribosome, inhibit the growth of the peptide chain and affect the synthesis of bacterial proteins. It is used for dysentery, trachoma, conjunctivitis, pneumonia, otitis media, skin suppurative infection, etc. It is also used for the treatment of amoebic enteritis and intestinal infection. However, when t...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/314G01N21/78
Inventor 栾倩干宁周游
Owner NINGBO UNIV
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