Preparation method and application of boron and nitrogen-doped green fluorescent carbon dots

Abstract

The invention discloses a preparation method of boron and nitrogen-doped green fluorescent carbon dots. The preparation method comprises the following steps: firstly, preparing a phenylboronic acid solution from deionized water and then mixing the mixed solution with anhydrous ethylenediamine; secondly, introducing nitrogen to react and then cooling to room temperature to obtain a boron and nitrogen-doped carbon quantum dot solution, thirdly, preserving the boron and nitrogen-doped carbon quantum dot solution at low temperature for later use and using carbon quantum dots for selective detection of horse radish peroxidase (HRP). The invention aims at providing the preparation method of the boron and nitrogen-doped green fluorescent carbon dots; the boron and nitrogen-doped green fluorescent carbon dots are prepared by using a hydrothermal method; the obtained carbon quantum dots with spherical structures have the advantages of uniform size and relatively-small average particle size; agglomeration of the carbon quantum dots also can be avoided by boric acid functional groups on the surfaces of the carbon quantum dots. The method has the advantages of relatively-simple synthesis process, economy, greenness and environment friendliness; in addition, the synthetic carbon quantum dots have a relatively-high fluorescent quantum yield; meanwhile, the carbon quantum dot solution has better selectivity to HRP protein.

Description

technical field [0001] The invention belongs to the technical fields of functional nanomaterial preparation technology and biological analysis and detection technology, and specifically relates to a preparation method and application of boron-nitrogen-doped green fluorescent carbon dots. Background technique [0002] The particle size of carbon dots (also known as carbon quantum dots) is less than 10nm. It is a new type of nanomaterial composed of carbonaceous skeleton and surface groups, and it is a kind of spherical carbon particles. Compared with metal quantum dot materials, carbon quantum dots have the advantage of low toxicity, so it is less harmful to the environment. In addition, it also has the characteristics of good biocompatibility and easy functionalization. [0003] There are many methods for preparing carbon quantum dots, which are generally divided into two categories: top-down method and bottom-up method. Top-down method: refers to the synthesis of carbon q...

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Problems solved by technology

[0004] Various synthetic methods have different advantages and disadvantages: arc discharge method, strong acid oxidation synthetic process is relatively complex and unfavorable for the collection of ten products; laser ablation synthetic process is complicated, the instrument used is expensive, not economical enough; The multi-methods are hydrothermal method, microwave digestion method, ultrasonic oscillation method, solvothermal method and template method. The synthesis process of these methods is relatively simple, economical and green, and the synthetic carbon quantum dots have a high fluorescence quantum yield.

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