High-purity BSA (bovine serum albumin) extraction method

Abstract

The invention provides a high-purity BSA (bovine serum albumin) extraction method. The method comprises steps as follows: a bovine serum solution is centrifugally separated, BSA is extracted through heating, primary dialysis purification is performed, secondary anion exchange chromatography purification is performed, and finally, a product is desalted, concentrated, filtered and sterilized. The method is short in technological process, simple to operate and high in production efficiency, furthermore, without application of an organic solvent, protein aggregation or denaturation is prevented, environmental pollution and unsafe factors in a production process can be reduced, product purity is improved, and the product can be widely applied to the fields of medical treatment, biochemistry and the like.

Description

technical field [0001] The invention relates to the technical field of protein purification, in particular to a high-purity bovine serum albumin extraction method. Background technique [0002] Protein is the material basis of life. Serum albumin is the most abundant protein in plasma. It can combine with many endogenous and exogenous compounds to play the role of storage and transformation. In the serum-free culture of animal cells, adding albumin can play the role of physiological and mechanical protection and carrier. Bovine serum albumin (BSA), also known as the fifth component, is a globulin in bovine serum, containing 583 amino acid residues, with a molecular weight of 66.43kDa and an isoelectric point of 4.7. Biochemical reagents widely used in other fields. [0003] At this stage, the extraction of bovine serum albumin mainly has the following three methods. The salting-out method is obtained by ammonium sulfate fractional precipitation and refined by octanoic aci...

AI Technical Summary

Problems solved by technology

The salting-out method is obtained by ammonium sulfate fractional precipitation and refined by octanoic acid treatment. This method is the most traditional production method, but its process is cumbersome and the production efficiency is not high; the organic solvent precipitation method utilizes the low dielectric constant properties of ethanol and The solubility of protein in different concentrations of ethanol is different under certain temperature, pH, ionic strength and concentration conditions, so as to realize the separation of plasma proteins. This method can produce a variety of plasma proteins, but the purity of the proteins is not high; the heat shock method is Take bovine blood, anticoagulate it with sodium citrate and centrifuge to obtain plasma, heat it, add sodium octanoate, add ethanol after adjusting the pH value, and then obtain bovine serum albumin after multi-stage treatment. In this method, some chemical substances will remain in serum albumin. Reagents, resulting in lower purity of bovine serum albumin