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Method for increasing synthetic yield of microbial oils of trichosporon cutaneum with steroidal compounds

The invention relates to a technology of Trichosporium dermoides and steroid compounds, which is applied in the field of microbial oil synthesis and can solve the problems of unstable source of raw materials for biodiesel production, complex components and large price fluctuations.

Active Publication Date: 2017-12-08
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because its raw materials mainly come from gutter oil, vegetable oil leftovers, etc., the source of raw materials for biodiesel production is unstable, the composition is complex, and the price fluctuates greatly, which greatly limits the normal production and supply of biodiesel.

Method used

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  • Method for increasing synthetic yield of microbial oils of trichosporon cutaneum with steroidal compounds
  • Method for increasing synthetic yield of microbial oils of trichosporon cutaneum with steroidal compounds
  • Method for increasing synthetic yield of microbial oils of trichosporon cutaneum with steroidal compounds

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Experimental program
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Effect test

Embodiment 1

[0028] Materials and Methods

[0029] Strain

[0030] Trichosporon cutaneum (Trichosporon cutaneum) was provided by the Allen McDelmid Institute of Renewable Energy, China Three Gorges University.

[0031] experimental method

[0032] Strain activation

[0033] Dip a small amount of bacterial liquid from the original bacterial species with an inoculating loop and inoculate it on the slope, and incubate at 30°C for 3 days.

[0034] Determination of the growth curve of corticospora

[0035] Pick a colony from the activated slant and inoculate it into 100mL seed liquid culture medium, culture at 28℃, 200rpm, take out a small amount of bacterial liquid every 2h, take the uninoculated seed liquid as a blank, and use a spectrophotometer with a wavelength of 600nm Measure the biomass and record the absorbance value. Take the culture time (h) as the abscissa and the absorbance value (OD 600 ) Is the ordinate to draw the yeast growth curve.

[0036] Seed liquid preparation

[0037] Pick a colony f...

Embodiment 2

[0061] A method for increasing the production of bio-oil of Trichosporium cormellitica. The colonies are picked from the activated slope and inoculated into the seed liquid culture medium (the seed culture medium is calculated as the following weight percentage, with 1.1 kg of sucrose, yeast Extract 0.8kg, malt extract 0.6kg, magnesium sulfate 0.07kg, disodium hydrogen phosphate 0.8kg, potassium dihydrogen phosphate 0.3kg, deionized water 96.33kg) culture for 18 hours, inoculate 10% of the inoculum into the fermentation culture Base (fermentation medium is as follows according to weight percentage, glucose 4kg, yeast extract 0.5kg, malt extract 0.3kg, magnesium sulfate 0.04kg, disodium hydrogen sulfate 0.3kg, potassium dihydrogen phosphate 0.09kg, deionized water 94.77kg.) Add 12g of prednisone acetate to the fermentation medium before culturing in a 100L fermentor. After culturing at 30°C for 30 hours, add 12g of prednisone acetate, and incubate for another 20 hours. Centrifuge...

Embodiment 3

[0063] A method for increasing the production of bio-oil of Trichosporium cormellitica. The colonies are picked from the activated slope and inoculated into the seed liquid culture medium (the seed culture medium is calculated as the following percentage by weight, with 2 kg of sucrose, yeast soaking Ointment 0.3kg, malt extract powder 0.7kg, magnesium sulfate 0.06kg, disodium hydrogen phosphate 0.7kg, potassium dihydrogen phosphate 0.07kg, deionized water 96.17kg), cultivate for 20 hours, and inoculate the fermentation culture with 10% of the inoculum Base: glucose 4kg, yeast extract 0.4kg, malt extract powder 0.7kg, magnesium sulfate 0.04kg, disodium hydrogen sulfate 0.6kg, potassium dihydrogen phosphate 0.15kg, deionized water 94.11kg. Add 15g of dexamethasone to the fermentation medium before culturing in a 100L fermentor. After culturing for 30 hours at 30°C, add 15g of dexamethasone, and then incubate for another 20 hours. Centrifuge at 8,500rpm for 10min to collect the ba...

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Abstract

The invention provides a method for increasing synthetic yield of microbial oils of trichosporon cutaneum with steroidal compounds. The method comprises the following steps: culturing a seed liquid specifically as follows: using an inoculating ring for dipping bacterial liquid from trichosporon cutaneum strains to be inoculated to an inclined surface for cultivation, and picking a bacterial colony from the activated inclined surface to be inoculated to a seed liquid culture medium for cultivation; then, inoculating the seed liquid to a fermentation culture medium containing steroidal compounds for cultivation, centrifuging and collecting thalli, placing into a drying oven for drying; adding hydrochloric acid to the dried thalli, and standing in a boiling water bath; rapidly cooling to a normal temperature and then adding twice the volume of chloroform-methanol solution; mixing uniformly, then standing at a room temperature and centrifuging; extracting to obtain a chloroform layer; adding chloroform to supernatant; shaking uniformly, centrifuging, and extracting for a second time to obtain another chloroform layer; combining the chloroforms; rotating, evaporating and removing chloroform to obtain the oil. The added steroidal compounds have no influence on varieties of the microbial oils in the trichosporon cutaneum, and have components similar to rapeseed oil and other vegetable oils; the contents of oleic acid and linoleic acid in oil fatty acid are increased by 21.6% and 32.3%, respectively.

Description

Technical field [0001] The invention relates to a microbial oil synthesis, in particular to a method for improving the synthesis yield of microbial oil of Trichosporon corticospora by using steroid compounds, and belongs to the field of bioenergy. Background technique [0002] In the face of increasingly depleted fossil fuels and the continuous deterioration of the environment, it has become an inevitable trend to seek new and renewable clean energy. Biodiesel is a clean and renewable energy source that can directly replace fossil fuels for diesel engine combustion. It has been widely promoted in my country in the past ten years. However, because its raw materials mainly come from waste oil, vegetable oil scraps, etc., the raw material source of biodiesel production is unstable, the composition is complicated, and the price fluctuates greatly, which greatly restricts the normal production and supply of biodiesel. Therefore, the development of stable, clean and renewable biodies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6463
Inventor 龚大春许鹏飞田毅红郭金玲吕育财
Owner CHINA THREE GORGES UNIV
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