Method and application for solid state fermentation of ginkgo leaves by using eurotium cristatum
A technology of solid-state fermentation of S. coronis, which is applied in the fields of application, botany equipment and methods, and horticulture, and can solve the problem of low spore count of S. coronis, lack of fermentable carbon source, nitrogen source, and the lack of fermentable carbon and nitrogen sources. The problem of limited growth of cystic fungus can achieve the effect of benefiting absorption, improving antioxidant activity and improving feeding effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0031] The activation of P. cristatum and the preparation of spore suspension:
[0032] Separation and purification: Take out the bacterial species stored at minus 20°C, use the sterilized and cooled inoculation loop to pick out the golden yellow colonies on the aseptic operation table, and inoculate the freshly prepared PDA by streaking the plate Medium. 28℃, relative humidity 85%, constant temperature and humidity culture. After cultured until golden yellow closed cysts grow on the plate (about 72h), use the sterilized inoculation loop to pick out the golden yellow colonies on the edge, and inoculate the strains on the new PDA. Repeat the above operation 2 to 3 times.
[0033] Preparation of spore suspension: Put 20-30 glass beads in a Erlenmeyer flask filled with sterile water, pick the PDA plate cultured for 72h, and transfer to sterile water, shake the shaker fully, and shake the condition It is 28°C, 220rpm, 45min. Use a hemocytometer to count the spores and adjust the co...
Example Embodiment
[0034] Example 1
[0035] (1) The freshly picked ginkgo biloba leaves are removed from impurities, washed, dried in an electric blast drying oven at 40°C for 6 hours, crushed and sieved to a size of 40 mesh with a grinder, to obtain ginkgo biloba powder, take 8.5g and place it in 100mL In a conical flask, the thickness should cover the bottom of the conical flask;
[0036] (2) Dry the ginkgo biloba in an electric blast drying oven at 60°C for 5 hours, pulverize and sieve to a size of 40 mesh by a pulverizer, to obtain whole ginkgo biloba powder. Add 0.675g of Ginkgo biloba powder to a 100mL Erlenmeyer flask, then add 5g of pH 4.5 phosphate buffer solution, mix the Ginkgo biloba powder, Ginkgo biloba powder and the buffer solution, and seal with a sterile parafilm, 121℃ High temperature and high pressure sterilization for 20 minutes, the solid-state fermentation medium for ginkgo biloba leaves is obtained after cooling for use;
[0037] (3) Under aseptic conditions, the solid-state ...
Example Embodiment
[0038] Example 2
[0039] (1) The freshly picked Ginkgo biloba leaves are removed from impurities, washed, dried in an electric blast drying box at 40°C for 6 hours, crushed with a pulverizer and sieved to a size of 40 meshes to obtain Ginkgo biloba powder, take 8.0g and place it in 100mL In a conical flask, the thickness should cover the bottom of the conical flask;
[0040] (2) Dry the bean cake at 60° C. for 5 hours in an electric heating blast drying box, pulverize and sieve to a size of 40 mesh by a pulverizer to obtain bean cake powder. Add 0.735g of soybean meal powder to a 100mL Erlenmeyer flask, then add 2.5g of phosphate buffer solution with pH 4.0, mix the Ginkgo biloba powder, soybean meal powder and buffer solution, seal with a sterile parafilm, and heat and pressure at 121℃. Bacteria for 20 minutes, the solid-state fermentation medium for ginkgo biloba leaves is obtained and cooled for use;
[0041] (3) Under aseptic conditions, inoculate the solid fermentation medium...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap