Liposome enhanced hydrogel and application thereof

A hydrogel and liposome technology, applied in the field of biomedicine, can solve problems such as difficult to realize hydrogel performance-enhancing drug regulated release, hydrogel strength decrease, skin irritation, etc., to improve comprehensive mechanical properties, reduce Small burst, evenly distributed effect

Inactive Publication Date: 2017-12-22
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, carbon nanomaterials have embryotoxicity and respiratory organ toxicity, and have a certain stimulating effect on the skin
The study also found that when microspheres, carbon nanotubes, etc. are combined with hydrogels, there will be a certain interface separation effect, which will directly cause the strength of hydrogels to decrease, and it is difficult to achieve enhanced hydrogel performance and controlled drug release.

Method used

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  • Liposome enhanced hydrogel and application thereof
  • Liposome enhanced hydrogel and application thereof
  • Liposome enhanced hydrogel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, drug-loaded liposome preparation

[0075] Considering that gemcitabine hydrochloride is a hydrophilic small molecule drug, reverse evaporation method was adopted to prepare gemcitabine hydrochloride liposomes. Weigh 200mg lecithin and 50mg cholesterol, dissolve in 3mL anhydrous ether. After being fully dissolved, the ether solution was transferred to a 250mL eggplant-shaped bottle. Weigh 50 mg of gemcitabine hydrochloride and dissolve it in 2 mL of deionized water to prepare a gemcitabine hydrochloride aqueous solution with a concentration of 25 mg / mL. Under the stirring condition of 500 rpm / min, a total of 1 mL of gemcitabine hydrochloride aqueous solution was added dropwise to the above-mentioned eggplant-shaped bottle, and after the addition was completed, the water bath was sonicated for 3 minutes to obtain a stable emulsion. Evaporate the obtained emulsion in a water bath at 10°C under reduced pressure to remove the organic solvent to obtain a gel-l...

Embodiment 2

[0076] Embodiment 2, drug-loaded liposome preparation

[0077] Paclitaxel liposomes were prepared by thin film dispersion method, as follows: Weigh 80 mg lecithin, 20 mg cholesterol and 6 mg paclitaxel into an eggplant bottle, and then add an appropriate amount of chloroform (the concentration of the dissolved lipid material is 2-5 mg / mL) to fully dissolve. The chloroform solution was placed in a 40° C. water bath, and the organic solvent was removed by rotary evaporation under reduced pressure, forming a honeycomb thin film on the inner wall of the eggplant-shaped bottle. Add 20 mL of deionized water preheated to 60° C. into the eggplant-shaped bottle, and then magnetically stir in a water bath at 60° C. for 1.5 h at a speed of 200 rpm / min. The obtained product was subjected to ultrasonic treatment, the ultrasonic time was 3 minutes, the ultrasonic power was 40%, and the operation was 2 seconds and the stop was 1 second. After the ultrasound was completed, the paclitaxel lip...

Embodiment 3

[0078] Embodiment 3, drug-loaded liposome preparation

[0079] Thrombin liposomes were prepared by reverse evaporation method as follows: 200 mg lecithin and 50 mg cholesterol were weighed and dissolved in 3 mL anhydrous ether. After being fully dissolved, the ether solution was transferred to a 250mL eggplant-shaped bottle. The thrombin freeze-dried powder was prepared into a thrombin solution with a concentration of 1000D / mL. Under the stirring condition of 500rpm / min, a total of 1mL of the thrombin solution was added dropwise to the eggplant-shaped bottle. A stable emulsion is obtained. The obtained emulsion was evaporated in a water bath at 10° C. under reduced pressure, and the organic solvent was removed to obtain a gel-like product. Add 4 mL of deionized water preheated to 37°C into the eggplant-shaped bottle, and perform ultrasonic treatment after hydration for 1 hour. After the ultrasound is finished, pass through 0.45 μm and 0.22 μm microporous membranes successiv...

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Abstract

The invention relates to the field of biomedicine, and provides liposome enhanced hydrogel and application thereof. The enhanced hydrogel is mainly prepared from hydrogel materials or blank or medicine carrying liposome through blending crosslinking; the hydrogel materials are selected from hyaluronic acid aquagel, cellulose aquagel, alginate aquagel, collagen aquagel, polyester aquagel, polyvinyl alcohol aquagel, collagen grafted polyacrylate aquagel, hyaluronic acid grafted N-isopropylacrylamideaquagel or unsaturated methacrylate modified gelatin; the addition quantity of the liposome is 1 to 150mg / mL of hydrogel system reclaimed water. The hydrogel provided by the invention has the advantages that the mechanical property is enhanced; the load and regulation and control release of different medicine can be realized, so that the multifunction of tissue repair and disease treatment is realized.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a liposome-enhanced hydrogel and its application. Background technique [0002] Hydrogel is a kind of polymer material with a three-dimensional network structure formed by introducing some hydrophobic groups and hydrophilic residues into the network crosslinked structure of water-soluble polymers, in which the hydrophilic residues and The water molecules combine to wrap the water molecules inside the network structure, and the hydrophobic residues swell with water. Hydrogels have the advantages of high water content, good viscoelasticity, soft texture, and good biocompatibility, making them widely used in biomedical fields such as scaffold coating, cell carrier, tissue engineering, and drug carrier. [0003] As a drug carrier, the current drug loading methods of hydrogels are mainly direct blending or carrier compounding. Among them, the hydrogel direct blending drug loadin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K9/06A61K47/36A61K47/42A61K47/34A61L27/20A61L27/24A61L27/18A61L27/52A61L27/54A61L27/50
CPCA61K9/06A61K9/127A61K47/34A61K47/36A61K47/42A61L27/18A61L27/20A61L27/222A61L27/24A61L27/50A61L27/52A61L27/54A61L2300/416A61L2430/02C08L5/08C08L1/02C08L89/00C08L29/04C08L5/04
Inventor 崔文国程若昱相宜
Owner SUZHOU UNIV
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