Eucommia polysaccharide with immunosuppression effect and preparation method and application thereof

Abstract

The invention discloses eucommia polysaccharide with an immunosuppression effect and a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: taking an eucommia medicinal material for crushing and alcohol extraction, and concentrating alcohol extract to obtain extract for standby use; volatilizing ethanol from drug residues, extracting the drug residues with water and concentrating; performing alcohol precipitation on the obtained extract, standing overnight, centrifuging, removing supernatant, dissolving precipitate with an appropriate amount of water, concentrating under reduced pressure and evaporating to remove the ethanol in the precipitate; evaporating a sample, dissolving the sample with water, and removing proteins with Sevag method; slightly concentrating a sample solution with proteins removed, transferring the sample solution to a dialysis bag with molecular weight cutoff of 7000, dialyzing with water for 48h, dialyzing with distilled water for 48h, merging dialysate, concentrating and freeze-drying to obtain a a yellowish brown solid, namely the eucommia polysaccharide with the immunosuppression effect. Compared with traditional chemical immunosuppressants, the eucommia polysaccharide has the following advantages: small toxic side effects; no dependence; less raw materials and relatively simple preparation process.

Description

technical field [0001] The invention belongs to the technical field of medicinal preparations, and particularly relates to a Eucommia ulmoides polysaccharide with immunosuppressive effect and a preparation method and application thereof. Background technique [0002] Immunosuppressive drugs are drugs that inhibit the immune response of the body. They can inhibit the proliferation and function of cells related to the immune response (macrophages such as T cells and B cells), and can reduce the antibody immune response. Immunosuppressants are mainly used for organ transplantation to resist rejection, rheumatic diseases and autoimmune diseases such as rheumatoid arthritis, lupus erythematosus, dermatomycosis, membranous nephritis, inflammatory bowel disease and autoimmune hemolytic anemia. [0003] There are five main types of commonly used immunosuppressants: (1) glucocorticoids, such as cortisone and prednisone; (2) microbial metabolites, such as cyclosporine and taupomycin; ...

AI Technical Summary

Problems solved by technology

[0009] Traditional immunosuppressants such as cyclophosphamide and cyclosporine A have played a very important role in organ transplantation and autoimmune diseases, but these drugs have disadvantages such as poor selectivity and large toxic and side effects, while new chemical immunosuppressants The high price of inhibitors limits its wide application in clinical practice. As a new type of immunosuppressant, the polysaccharide extract of traditional Chinese medicine has the characteristics of wide range of pharmacological effects, small side effects and no dependence compared with traditional immunosuppressants.

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