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Synthesis method of fluorescent probe with high fluorescence intensity and method for detecting beta receptor stimulant by using fluorescent probe

A technology of receptor agonist and fluorescent probe, which is applied in the direction of fluorescence/phosphorescence, material analysis through optical means, and measurement devices, etc., can solve the problems of fluorescence detection method limitations and weak fluorescence intensity, and achieve stable detection results and responsiveness. The effect of high speed and high productivity

Active Publication Date: 2017-12-22
甘肃省商业科技研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, depending on the structure of β-receptor agonists, not every substance has fluorescence, such as clenbuterol, formoterol, phenylethanolamine A and other substances with weak or no fluorescence intensity.
Therefore, fluorescence detection methods still have great limitations.

Method used

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  • Synthesis method of fluorescent probe with high fluorescence intensity and method for detecting beta receptor stimulant by using fluorescent probe
  • Synthesis method of fluorescent probe with high fluorescence intensity and method for detecting beta receptor stimulant by using fluorescent probe
  • Synthesis method of fluorescent probe with high fluorescence intensity and method for detecting beta receptor stimulant by using fluorescent probe

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Experimental program
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Effect test

Embodiment 1

[0030] The synthetic method of the fluorescent probe of embodiment 1 high fluorescence intensity is as follows:

[0031] Weigh the reactant 2,3-naphthalene diamine 8g, the reactant benzoyl chloride 7g, the catalyst and the solvent 1-butyl-3-methylimidazolium hexafluorophosphate 1g respectively in a round bottom flask according to the mass ratio, and stir evenly 30min, a mixed solution was obtained. The above mixed solution was extracted three times with 15 g of aqueous hydrochloric acid solution with a pH value of 2. Combine the hydrochloric acid solutions. Use 10% sodium hydroxide aqueous solution to adjust the pH value of the hydrochloric acid solution to 10, and a white crystal precipitate will be precipitated at this time. Filter, washing the filter cake several times with cold water. The filter cake was recrystallized with 50% ethanol aqueous solution and dried to obtain white needle-like crystals, namely 2-phenylnaphthimidazole, with a yield of 81.5%. Take 7g of chlo...

Embodiment 2

[0032] The synthetic method of the fluorescent probe of embodiment 2-high fluorescence intensity is as follows:

[0033]Weigh 8.2 g of the reactant 2,3-naphthalene diamine, 7.2 g of the reactant benzoyl chloride, and 1.2 g of the catalyst and solvent 1-butyl-3-methylimidazolium hexafluorophosphate in a round bottom flask according to the mass ratio , and stirred evenly for 30 min to obtain a mixed solution. The above mixed solution was extracted three times with 15.2 g of an aqueous hydrochloric acid solution with a pH value of 2. Combine the hydrochloric acid solutions. Use 10% sodium hydroxide aqueous solution to adjust the pH value of the hydrochloric acid solution to 10, and a white crystal precipitate will be precipitated at this time. Filter, washing the filter cake several times with cold water. The filter cake was recrystallized with 50% ethanol aqueous solution and dried to obtain white needle-like crystals, namely 2-phenylnaphthimidazole, with a yield of 81.5%. T...

Embodiment 3

[0034] The synthetic method of the fluorescent probe of embodiment 3-high fluorescence intensity is as follows:

[0035] Weigh 7.8g of the reactant 2,3-naphthalene diamine, 6.8g of the reactant benzoyl chloride, and 0.8g of the catalyst and solvent 1-butyl-3-methylimidazolium hexafluorophosphate in a round-bottomed flask according to the mass ratio , and stirred evenly for 30 min to obtain a mixed solution. The above mixed solution was extracted three times with 14.8 g of aqueous hydrochloric acid solution with a pH value of 2. Combine the hydrochloric acid solutions. Use 10% sodium hydroxide aqueous solution to adjust the pH value of the hydrochloric acid solution to 10, and a white crystal precipitate will be precipitated at this time. Filter, washing the filter cake several times with cold water. The filter cake was recrystallized with 50% ethanol aqueous solution and dried to obtain white needle-like crystals, namely 2-phenylnaphthimidazole, with a yield of 81.5%. Take...

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Abstract

The invention discloses a synthesis method of a fluorescent probe with high fluorescence intensity and a method for detecting a beta receptor stimulant by using the fluorescent probe and belongs to the field of detection of food safety. The synthesis method of the fluorescent probe with high fluorescence intensity takes 2,3-naphthylenediamine and benzoyl chloride as reactants and takes 1-butyl-3-methylimidazolium hexafluorophosphate as a catalyst to prepare the fluorescent probe. The fluorescent probe is high in fluorescence intensity, simple and convenient to synthesize and high in specificity of reaction of amino compounds; when the generated fluorescent probe is used for detecting the beta receptor stimulant, the fluorescent probe is rapid in derivation speed and high in fluorescence intensity; the detection sensitivity on the drugs is improved; the detection cost is reduced.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a method for synthesizing a fluorescent probe with high fluorescence intensity and a method for detecting beta receptor agonists using it. Background technique [0002] β-receptor agonists are widely used in poultry breeding to promote animal growth and increase lean meat yield. However, long-term use will be enriched in animal tissues, and human consumption of the animal tissues will produce very serious side effects, thereby endangering human health. In the field of national food safety, the detection of three traditional β-receptor agonists, ractopamine, albuterol, and clenbuterol, in animal-derived products is mandatory, but there is no corresponding detection standard for new β-receptor agonists, such as : Cimaterol, phenylethanolamine A, terbutaline, formoterol, etc. The above-mentioned β-receptor agonists all have a common characteristic structure, that i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N30/02
CPCG01N21/6428G01N30/02
Inventor 周鑫魁洪霞钱滢文高志莹王杰斌何海宁
Owner 甘肃省商业科技研究所有限公司
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