Culture medium for producing D-pantolactone hydrolase by fermentation through fusarium oxysporum mycete

A technology of pantolactone and fermentation medium, which is applied in the field of medium for producing D-pantolactone hydrolase, can solve the problems of high cost of fermentation raw materials, increased production cost, and low fermentation biomass, and achieves Improve the yield of fermentation enzymes, improve the quality, promote the effect of mycelial growth and metabolism

Active Publication Date: 2017-12-29
NINGXIA KINGVIT PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1 The cost of fermentation raw materials is high
In general, in the culture medium of D-pantolactone hydrolase, peptone and casein should be added as nitrogen source substances. The price of such raw materials is relatively high, resulting in increased production costs.
[0007] 2 In the traditional enzyme production medium prescription, there is a lack of suitable inducers for enzyme synthesis and enzyme production accelerators, resulting in low fermentation biomass, generally around 7.2g dry mycelium / L fermentation broth; enzyme activity is not high, generally at About 1.0U / g dry mycelium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Seed culture: the effective volume of seed medium is 2m 3 .

[0030] Seed medium: glycerin 20kg, corn gluten powder 24kg, corn steep liquor 6kg, yeast extract powder 12kg, corn flour 40kg, glucose 40kg, (NH 4 ) 2 SO 4 18kg, light calcium carbonate 12kg, phytic acid 0.12kg.

[0031] First, sterilize the seed medium at 121°C, keep the pressure for 30 minutes, cool to 30°C, and keep the pressure with sterile air. The quality requirements of the sterilized seed medium are: amino nitrogen 64mg / 100ml, total sugar 3.2g / 100ml, pH6.9. Then, under the protection of the flame, the mycelium of the mother bottle of Fusarium oxysporum that has been cultivated is inserted into the seed medium according to the inoculation amount of 0.1L for seed cultivation. The seed cultivation conditions are: tank pressure 0.03-0.05MPa; tank temperature 28 ±0.5℃; air flow 180m 3 / h; the stirring speed is 220r / min, until the cell concentration is 25.2%, the pH value is 6.6, and the incubation ...

Embodiment 2

[0037] Seed culture: the effective volume of seed medium is 2m 3 .

[0038]Seed medium: glycerin 24kg, corn gluten powder 26kg, corn steep liquor 8kg, yeast extract powder 14kg, corn flour 42kg, glucose 42kg, (NH 4 ) 2 SO 4 20kg, light calcium carbonate 14kg, phytic acid 0.16kg.

[0039] First, sterilize the seed medium at 121°C, keep the pressure for 30 minutes, cool to 30°C, and keep the pressure with sterile air. The quality requirements of the sterilized seed medium are: amino nitrogen 69mg / 100ml, total sugar 3.4g / 100ml, pH7.3. Then, under the protection of the flame, the mycelium of the mother bottle of Fusarium oxysporum that has been cultivated is inserted into the seed medium according to the inoculation amount of 0.1L for seed cultivation. The seed cultivation conditions are: tank pressure 0.03-0.05MPa; tank temperature 28 ±0.5℃; air flow 180m 3 / h; the stirring speed is 220r / min, until the cell concentration is 26.6%, the pH value is 6.9, and the incubation t...

Embodiment 3

[0045] Seed culture: the effective volume of seed medium is 2m 3 .

[0046] Seed medium: glycerin 28kg, corn gluten powder 28kg, corn steep liquor 10kg, yeast extract powder 16kg, corn flour 44kg, glucose 44kg, (NH 4 ) 2 SO 4 22kg, light calcium carbonate 16kg, phytic acid 0.2kg.

[0047] First, sterilize the seed medium at 121°C, keep the pressure for 30 minutes, cool to 30°C, and keep the pressure with sterile air. The quality requirements of the sterilized seed medium are: amino nitrogen 75mg / 100ml, total sugar 3.5g / 100ml, pH7.4. Then, under the protection of the flame, the mycelium of the mother bottle of Fusarium oxysporum that has been cultivated is inserted into the seed medium according to the inoculation amount of 0.1L for seed cultivation. The seed cultivation conditions are: tank pressure 0.03-0.05MPa; tank temperature 28 ±0.5℃; air flow 180m 3 / h; the stirring speed is 220r / min, until the cell concentration is 27.6%, the pH value is 6.9, and the incubation ...

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Abstract

The invention relates to a culture medium for producing D-pantolactone hydrolase by fermentation through fusarium oxysporum mycete. The culture medium for producing D-pantolactone hydrolase by fermentation through fusarium oxysporum mycete comprises a seed culture medium and a fermentation culture medium; phytic acid is used in the culture medium, the activity of strains is improved, growth and metabolism of mycelia are promoted, the quality of a seed solution is improved, and the fermentative enzyme production capacity is improved; and furfural is used in the fermentation culture medium, fermentation is induced favorably to generate the D-pantolactone hydrolase, and the biomass of fermentation is improved. The biomass of fermentation of the culture medium is greater than or equal to 7.8 g/L dry mycelium. Compared with the domestic technology, the culture medium for producing the D-pantolactone hydrolase by fermentation through the fusarium oxysporum mycete has the advantages that the biomass of fermentation is increased by 8.3% or above; and the activity of production of the D-pantolactone hydrolase reaches 1.29 mu/g (dry mycelium) or above, and the enzyme activity is increased by 30% or above.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a medium for fermenting and producing D-pantolactone hydrolase by using fusarum oxysporm. Background technique [0002] Calcium D-pantothenate, also known as VB5, chemical name D-N-(2,4-dihydroxy-3,3-dimethylbutyryl)-β-alanine calcium), relative molecular weight: 476.54. Calcium pantothenate can be divided into three types: DL-type (mixed body), D-type (dextral body), and L-type (left-handed body), of which only the D-type (dextral body) has biological activity. Calcium D-pantothenate enters the human body to release calcium and pantothenic acid, and pantothenic acid is the precursor of coenzyme A, which is then converted into coenzyme A to produce physiological effects, participate in the metabolism of carbohydrates, fats and proteins, and maintain normal physiology for humans and animals Trace substances indispensable to functions. Mainly used in medicine, food and feed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12N1/14C12R1/77
CPCC12N1/14C12N9/16
Inventor 裴立忠李谦张延梅沈晓鸿
Owner NINGXIA KINGVIT PHARMA
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