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Method for detecting concentration of dopamine in solution

A dopamine and concentration technology, applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of unsatisfactory rapid detection, large consumption of reagents, long analysis time, etc., and achieve simple and convenient detection process and improve sensitivity Good performance and repeatability

Active Publication Date: 2017-12-29
武汉市皮诺飞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for determining dopamine mainly include liquid chromatography, chemiluminescence, capillary electrophoresis, fluorescence technology, mass spectrometry and spectrophotometry, etc. Although these technologies are widely used, the instruments used are expensive, the consumption of reagents is large, and the analysis time is long Long, unable to meet the needs of on-site rapid detection

Method used

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  • Method for detecting concentration of dopamine in solution
  • Method for detecting concentration of dopamine in solution
  • Method for detecting concentration of dopamine in solution

Examples

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Effect test

Embodiment 1

[0034] A method for detecting dopamine concentration in a solution, comprising the steps of:

[0035] Step 1. Weigh 1g of sucrose and put it in a beaker, add 2mL of deionized water and 2mL of concentrated phosphoric acid to the beaker in sequence, and ultrasonicate the beaker for 5min to completely dissolve the sucrose; then put the beaker into a water bath at 80°C and heat it for 50min. Take out the beaker and let it cool down naturally. After the beaker is cooled to room temperature, add 6mL of ethylenediamine in an ice-water bath, stir evenly and form a paste; then add water to dissolve it, and dialyze the resulting solution with a dialysis bag with a shear molecular weight of 500 for 72 hours to obtain The first material, the second material obtained by freeze-drying the first material into a solid, and adding deionized water to the first material to obtain a solution with a concentration of 5 mg / mL to obtain a carbon dot solution;

[0036] Step 2, modifying the glassy car...

Embodiment 2

[0049] A method for detecting dopamine concentration in a solution, comprising the steps of:

[0050] Step 1. Weigh 1g of sucrose and put it in a beaker, add 2mL of deionized water and 2mL of concentrated phosphoric acid to the beaker in sequence, and ultrasonicate the beaker for 5min to completely dissolve the sucrose; then put the beaker into a water bath at 80°C and heat it for 50min. Take out the beaker and let it cool down naturally. After the beaker is cooled to room temperature, add 6mL of ethylenediamine in an ice-water bath, stir evenly and form a paste; then add water to dissolve it, and dialyze the resulting solution with a dialysis bag with a shear molecular weight of 500 for 72 hours to obtain The first material, the second material obtained by freeze-drying the first material into a solid, and adding deionized water to the first material to obtain a solution with a concentration of 5 mg / mL to obtain a carbon dot solution;

[0051] Step 2, modifying the glassy car...

Embodiment 3

[0061] A method for detecting dopamine concentration in a solution, comprising the steps of:

[0062] Step 1, modifying the glassy carbon electrode, the specific method is:

[0063] Step a, sequentially use 0.5μm, 0.3μm and 0.05μm α-A1 2 o 3The surface of the glassy carbon electrode is ground into a mirror surface by the powder, and the surface of the glassy carbon electrode is rinsed with ultrapure water, and then the glassy carbon electrode is placed in triple distilled water and ethanol for ultrasonic treatment. The completed glassy carbon electrode is cleaned with ultrapure water, and then placed in room temperature to dry to obtain a pretreated glassy carbon electrode;

[0064] Step b, take 1 mL of aminated reduced graphene oxide with a concentration of 3 mg / mL and ultrasonically treat it for 5 minutes, then use a pipette to pipette 5 μL of the amino-reduced graphene oxide after ultrasonic treatment, and add dropwise the pretreated aminated graphene oxide obtained in st...

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Abstract

The invention discloses a method for detecting the concentration of dopamine in a solution. The method comprises steps as follows: S1, a carbon dot solution is prepared for later use; S2, a glassy carbon electrode is modified with amination reduced graphene oxide and the carbon dot solution sequentially; S3, multiple PBS (poly(butylene succinate)) buffer solutions containing different concentrations of dopamine are prepared, the modified glassy carbon electrode is used as a work electrode, a three-electrode system is constituted, multiple buffer solutions are detected sequentially through the three-electrode system, oxidation peak current values corresponding to the multiple buffer solutions are recorded, and a relationship formula between the concentration of dopamine and the oxidation peak current values is established; S4, to-be-detected dopamine with unknown concentration is added to the PBS buffer solutions and detected through the three-electrode system in S3, oxidation peak current values are recorded, and the concentration of dopamine is solved according to the relationship formula. The method is simple and convenient in the detection process, high in sensitivity and low in limit of detection and can realize rapid detection of the concentration of dopamine in an actual sample.

Description

technical field [0001] The invention relates to the technical field of dopamine detection. More specifically, the present invention relates to a method for detecting the concentration of dopamine in a solution by differential pulse voltammetry based on aminated graphene quantum dots and carbon quantum dots modified electrochemical sensing. Background technique [0002] Dopamine (DA, 3,4-dihydroxyphenethylamine), an important catecholamine neurotransmitter, is ubiquitously present in mammalian brain tissue, and plays a role in renal function, cardiovascular function, central nervous system and endocrine system play an important role. The imbalance of dopamine content can lead to the occurrence of neurological diseases, such as Tourette's syndrome, schizophrenia, Parkinson's disease and so on. Additionally, dopamine is available directly intravenously, acting directly on the sympathetic nervous system and producing effects such as increasing heart rate and blood pressure. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3271G01N27/48
Inventor 肖琦黄珊李家文
Owner 武汉市皮诺飞生物科技有限公司
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