Microsatellite instability sequencing data analysis method and apparatus, and computer readable medium

A technology for sequencing data and instability, applied in the field of gene detection, can solve the problem of low sensitivity to microsatellite instability, and achieve the effect of improving detection sensitivity

Active Publication Date: 2017-12-29
GENESEEQ TECH INC
View PDF3 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] At the same time, the present invention can also solve the problem of low sensitivity in the routine process of determining whether a site is microsatellite instability based on MSI sequencing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microsatellite instability sequencing data analysis method and apparatus, and computer readable medium
  • Microsatellite instability sequencing data analysis method and apparatus, and computer readable medium
  • Microsatellite instability sequencing data analysis method and apparatus, and computer readable medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] The preliminary screening of embodiment 1 locus

[0123] In the following, the present invention demonstrates the application of the analysis process and the MSI site screening method with examples. We performed high-throughput sequencing on the tumor tissue samples of 2,000 Chinese individuals suffering from solid tumors and the corresponding negative control samples of whole blood. The target genomic interval of the detection was the whole exon region of 422 cancer-related genes, and Intronic regions of some common gene fusions. The detection uses the liquid phase capture method to enrich the target DNA. The sequencing process is a common next-generation sequencing method, and its brief description is as follows: extract DNA from tissue samples and whole blood samples of each patient to obtain genomic DNA; Fragmentation to 200-350 base pairs; end repair, addition of adenine, library adapter ligation and other operations on fragmented DNA molecules; obtained DNA libr...

Embodiment 2

[0130] Example 2 Data Analysis of the Unstable State of Microsatellite Sites

[0131] Whole blood samples from 100 healthy people in Example 1 and 163 colorectal cancer clinical samples that had undergone Promega PCR microsatellite detection were used to perform sequencing analysis of microsatellite loci, and the sequencing process was carried out on an illumina next-generation sequencer Carried on.

[0132] First, after processing the off-machine sequencing data of normal population and patient samples, reads that can cover 22 MSI loci are found, and then these reads are classified according to the number of single base repeats in the microsatellite loci. Classes and numbers accumulate. The main repeat unit types at each site corresponding to normal samples and patient samples were counted, and the population-dominated repeat unit types on all normal samples were counted. The average value and standard deviation of the main repeat unit types on the 22 MSI sites were counted...

Embodiment 3

[0142] The microsatellite instability analysis of embodiment 3 site combination

[0143] In order to demonstrate the superiority of the 22 microsatellite instability sites combined with biological indicators proposed by the present invention, the 22 microsatellite sites obtained by screening were compared with the 22 randomly selected sites in the remaining sites in the 96 candidate sites. sites, compared in terms of accuracy in defining microsatellite instability in patients.

[0144] Control Experiment 1: Figure 6 22 randomly selected microsatellite instability points (numbered in Table 2 are respectively 39, 88, 43, 34, 25, 56, 63, 69, 42, 32, 28, 45, 59, 98, 44 , 50, 85, 99, 67, 53, 52, and 66 MSI sites), only the analysis results obtained from the determination condition of "the number of main repeat unit types". The classification of MSS, MSI-L, and MSI-H in the figure is based on the judgment results of the Promega kit, and each data point is 163 clinical samples t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a microsatellite instability sequencing data analysis method and apparatus, and a computer readable medium. Whether a situation of microsatellite instability exists or not can be determined by utilizing an NGS sequencing result; the analysis calculation method can remarkably improve detection sensitivity under the condition of not reducing specificity; a stable or instable state of each MSI site can be assessed in a quick, automatic, high-flux, high-sensitivity and high-specificity manner; and in combination with states of all MSI sites in each sample, the sample is comprehensively assessed as MSS, MSI-L or MSI-H.

Description

technical field [0001] The present invention relates to the field of gene detection, in particular, the present invention relates to a method for analyzing detection results of microsatellite instability (MSI) indicator sites based on next-generation sequencing technology (NGS). The method is suitable for simultaneous analysis of one or more microsatellite sites indicating MSI status, accurately assessing the MSI status of patients, and providing theoretical basis and guidance for tumor diagnosis, prognosis, and formulation of clinical treatment plans. Background technique [0002] Microsatellite instability (Micro-Satellite Instability, or MSI) is a molecular phenotype, which is embodied in the presence of insertion or deletion mutations in the repeat sequence of microsatellite loci in the human genome, thereby producing alleles. These insertions or deletions are not corrected by DNA mismatch repair mechanisms (MMR) for a number of reasons. The phenomenon of MSI was first...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/22
CPCG16B30/00G16B20/20G16B20/40G16B40/30
Inventor 邵阳朱明赴常志力汪笑男闫重光吴舒雨包华童晓玲
Owner GENESEEQ TECH INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap